Separation technology for aqueous two-phase extraction of spirulina phycocyanin from polyvinylether phenylacetamide

A polyvinyl ether and phenylacetamide technology, which is applied in the fields of depsipeptides, peptides, chemical instruments and methods, can solve the problems of high price, poor selectivity of separating phycocyanin, and complicated process, and achieves low production cost and solves the problem of utilization. and high-value problems, the effect of high extraction selectivity

Inactive Publication Date: 2017-02-01
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reported patented technology of two-phase extraction and separation of phycocyanin for the preparation of algae phycocyanin, such as Chinese patent CN103880950A, uses expensive ionic liquids to form a polymer-water phase, which increases production costs. CN102993297A, C

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0016] Implementation example 1

[0017] (1) Preparation of Spirulina Broken Wall Clear Liquid

[0018] Take the dry powder of Spirulina platensis produced by Cibainian Biological Engineering Co., Ltd. in Dongtai City, Jiangsu Province, use deionized water as the extractant, the solid-liquid ratio of the algae powder and deionized water is 1:50, and freeze at -20℃4 After hours, it was thawed at 37°C. Repeated freezing and thawing for 4 times. The thawed cyanobacteria wall-broken liquid was centrifuged at 6000 rpm for 10 minutes in a centrifuge. The supernatant was taken as the wall-broken supernatant. The protein concentration in the supernatant was 1.0mg / ml, phycocyanin purity A 620 / A 280 It is 0.72mg / ml.

[0019] (2) Preparation of polyvinyl ether methanesulfonate

[0020] Put 100g polyethylene glycol PEG-1000 (purchased from Haian Petrochemical Plant) and 300ml of methanesulfonyl chloride (purchased from Jinan Zi'an Chemical Co., Ltd.) in a 1000ml round bottom flask with dichloro...

Example Embodiment

[0027] Example 2

[0028] (1) Preparation of spirulina wall breaking solution

[0029] Take fresh spirulina produced in Jiangsu Dafengci Bainian Biotechnology Co., Ltd., use 50mmol / L phosphate buffer as extractant, the solid-liquid ratio of spirulina to deionized water is 1:100, and freeze at -10℃ After 4 hours, it was thawed at 37°C and repeated freezing and thawing 5 times. The melted cyanobacteria wall-breaking liquid was centrifuged at 6000 rpm for 10 minutes in a centrifuge, and the supernatant was taken as the wall-breaking clear liquid. The protein concentration in the clear liquid was 3.1 mg / ml, and the phycocyanin purity A 620 / A 280 Is 0.5.

[0030] (2) Preparation of polyvinyl ether methanesulfonate

[0031] Put 100g polyethylene glycol PEG-3000 (purchased from Haian Petrochemical Plant) and 300ml of methanesulfonyl chloride (purchased from Jinan Zi'an Chemical Co., Ltd.) in a 1500ml round bottom flask with dichloromethane (purchased from Dongying Huibao) Chemical Co., Lt...

Example Embodiment

[0038] Example 3

[0039] (1) Preparation of spirulina wall breaking solution

[0040] Take the spirulina powder produced in the Chenghaiyuan Spirulina base in Lijiang, Yunnan, use 100mmol / L phosphate buffer as the extractant, the solid-liquid ratio of spirulina to deionized water is 1:150, and freeze at -20℃ for 4 hours Then, it was thawed at 37°C, and the freeze-thaw cycle was repeated 6 times. The melted cyanobacteria wall-breaking liquid is centrifuged at 6000 rpm for 10 minutes in a centrifuge, and the supernatant is taken as the wall-breaking clear liquid. The purity of the phycocyanin in the clear liquid is A 620 / A 280 It is 0.72, and the protein concentration of wall-breaking fluid is 5.6mg / ml mg / ml.

[0041] (2) Preparation of polyvinyl ether methanesulfonate

[0042] Put 100g polyethylene glycol PEG-3000 (purchased from Haian Petrochemical Plant) and 300ml of methanesulfonyl chloride (purchased from Jinan Zi'an Chemical Co., Ltd.) in a 2000ml round bottom flask with dichlo...

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PUM

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Abstract

The invention relates to a separation technology for aqueous two-phase extraction of spirulina phycocyanin from polyvinylether phenylacetamide, and belongs to the technical field of biochemical separation engineering. The separation technology comprises the steps of polyvinylether phenylacetamide preparation; two-phase extraction of phycocyanin; phycocyanin powder obtaining after concentration and freeze drying, wherein the protein recovery rate can reach 70 to 85 percent. The method is simple; spirulina wall breaking liquid is subjected to enrichment and separation by directly using the polyvinylether phenylacetamide; the purification process step is simplified; the selectivity is high; the spectrum purity of the phycocyanin obtained through separation achieves the effect that A620/A280 is greater than 2.5. Raw materials can use fresh spirulina or dry spirulina powder, so that a technology method for realizing high-value large-scale utilization of spirulina resources is provided.

Description

technical field [0001] The invention provides a preparation method of polyvinyl ether phenylacetamide, which is used for extracting phycocyanin from spirulina wall breaking liquid. It belongs to the technical field of biochemical separation engineering. Background technique [0002] Spirulina phycocyanin is present in the phycobilisomes of Spirulina. Phycobilisomes are supramolecular light-harvesting pigment complexes unique to red and cyanobacteria. Phycocyanin is bright blue in color and bright in color. It is the first choice of pure natural pigment for food, high-end eye shadow and lipstick. Phycocyanin can also regulate and synthesize a variety of important enzymes required by human metabolism, has obvious anti-oxidation and anti-inflammatory effects, regulates the human immune system, and enhances the function of the immune system. High-purity phycocyanin has strong fluorescence, and it is made of pure natural fluorescent reagents, which are used in research fields ...

Claims

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Application Information

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IPC IPC(8): C07K14/795C07K1/14
CPCC07K14/795
Inventor 王峰
Owner JIANGNAN UNIV
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