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Method for producing biodiesel from microalgae oil

A biodiesel, microalgae oil technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low biodiesel yield, low catalytic efficiency, long reaction time, etc., and achieve strong environmental adaptability. , improve the catalytic efficiency, the effect of large reaction interface

Inactive Publication Date: 2017-02-01
南宁华侨投资区政孙贸易有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing biodiesel production process generally has the following problems: low catalytic efficiency, low biodiesel yield, long reaction time, corrosion equipment, complex process, high energy consumption, high cost, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Preparation of Scenedesmus ellipsoides culture medium: Glucose 15mg / L, NaNO 3 2.4mg / L, KH 2 PO 4 1.6mg / L, chitosan 0.9mg / L, biogas slurry 6mg / L, f2 vitamin 2mL and seawater 1L;

[0027] A method for producing biodiesel with microalgae oil as raw material, comprising the following steps:

[0028] S1. Cultivation of Scenedesmus ellipsoides: use Scenedesmus ellipsoides as raw material, inoculate it into the culture medium at an inoculation density of 3 million cells / mL, and irradiate it with red and blue LED supplementary light for 16 hours / day, and the red light in the LED supplementary light The ratio with blue light is 1:1, the light intensity is controlled at 2000LX, the culture temperature is controlled at 23°C, cultured for 10 days, the density of Scenedesmus ellipsoides reaches 80 million cells / mL, and the cells of Scenedesmus ellipsoides are collected;

[0029] S2. Grease extraction: After concentrating and drying the Scenedesmus ellipsoidal cells collected in ...

Embodiment 2

[0034] Preparation of Scenedesmus ellipsoides culture medium: glucose 20mg / L, NaNO 3 2.3mg / L, KH 2 PO 4 1.8mg / L, chitosan 0.8mg / L, biogas slurry 8mg / L, f2 vitamin 3mL and seawater 1L;

[0035] A method for producing biodiesel with microalgae oil as raw material, comprising the following steps:

[0036]S1. Cultivation of Scenedesmus ellipsoides: use Scenedesmus ellipsoides as raw material, inoculate it into the culture medium at an inoculation density of 3 million cells / mL, and irradiate it with red and blue LED supplementary light for 14h / day, and the red light in the LED supplementary light The ratio with blue light is 1:2, the light intensity is controlled at 2500LX, the culture temperature is controlled at 24°C, cultured for 9 days, the density of Scenedesmus ellipsoides reaches 80 million cells / mL, and the cells of Scenedesmus ellipsoides are collected;

[0037] S2. Grease extraction: After concentrating and drying the Scenedesmus ellipsoidal cells collected in step S1...

Embodiment 3

[0042] Preparation of Scenedesmus ellipsoides culture medium: glucose 25mg / L, NaNO 3 2.2mg / L, KH 2 PO 4 2.0g / L, chitosan 0.6mg / L, biogas slurry 10mg / L, f2 vitamin 2mL and seawater 1L;

[0043] A method for producing biodiesel with microalgae oil as raw material, comprising the following steps:

[0044] S1. Cultivation of Scenedesmus ellipsoides: use Scenedesmus ellipsoides as raw material, inoculate into the culture medium at an inoculation density of 3 million cells / mL, and irradiate with red and blue LED supplementary light for 10h / day, and the red light in the LED supplementary light The ratio with blue light is 1:3, the light intensity is controlled at 3000LX, the culture temperature is controlled at 25°C, cultured for 10 days, the density of Scenedesmus ellipsoides reaches 80 million cells / mL, and the cells of Scenedesmus ellipsoides are collected;

[0045] S2. Grease extraction: After concentrating and drying the Scenedesmus ellipsoidal cells collected in step S1, th...

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Abstract

The invention discloses a method for producing biodiesel from microalgae oil. The method comprises the following steps: inoculating a culture medium with scenedesmus ovalternus, irradiating the culture medium by virtue of a red-blue double-color LED light-compensating lamp for 10-16h / day, conducting culture at 23-25 DEG C for 9-10 days and collecting scenedesmus ovalternus cells; and conducting wall-breaking treatment on the collected scenedesmus ovalternus cells by virtue of ultrasonic wave and extracting the scenedesmus ovalternus cells by virtue of an organic solvent so as to obtain the microalgae oil, adding the microalgae oil, methanol and a zeolite molecular sieve catalyst to a reactor according to a mass ratio of 1 to (6-8) to (0.02-0.05) and conducting an esterification reaction so as to obtain an esterification product, and separating and purifying the esterification product so as to obtain the biodiesel. The method provided by the invention, which adopts the scenedesmus ovalternus which is short in production cycle, wide in distribution, strong in photosynthesis and high in oil content as a raw material, can solve the problem on a plant oil raw material in biodiesel development. Meanwhile, the method also has the advantages of being simple in preparation process, high in catalysis efficiency, high in oil extracting rate, high in product yield and the like, so that a good foundation is laid for the industrial and large-scale production of the biodiesel.

Description

technical field [0001] The invention belongs to the technical field of biomass energy, and specifically relates to a method for producing biodiesel by using microalgae oil as a raw material. Background technique [0002] With the increasing depletion of fossil fuels and the sharp rise in oil prices, the search for renewable energy has become a solution to human survival and to maintain the sustainable development of the world economy. As a new alternative energy, biodiesel is developing rapidly in countries all over the world. Biodiesel refers to oil crops such as soybeans, rapeseed, cotton, palm, etc., wild oil plants and engineering microalgae and other aquatic vegetable oils, animal fats, and catering waste oils, etc. Renewable diesel fuel to replace petrodiesel. The advantages of biodiesel are not limited to renewability. Compared with existing diesel, it also has the following advantages: high cetane number, which makes the combustion performance better; better wetting...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C11C3/10C10L1/02C12R1/89
CPCC10L1/02C11C3/10C12P7/6463C12P7/649Y02E50/10
Inventor 黄健峻其他发明人请求不公开姓名
Owner 南宁华侨投资区政孙贸易有限公司
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