Method for producing biodiesel from microalgae oil
A biodiesel, microalgae oil technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low biodiesel yield, low catalytic efficiency, long reaction time, etc., and achieve strong environmental adaptability. , improve the catalytic efficiency, the effect of large reaction interface
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Embodiment 1
[0026] Preparation of Scenedesmus ellipsoides culture medium: Glucose 15mg / L, NaNO 3 2.4mg / L, KH 2 PO 4 1.6mg / L, chitosan 0.9mg / L, biogas slurry 6mg / L, f2 vitamin 2mL and seawater 1L;
[0027] A method for producing biodiesel with microalgae oil as raw material, comprising the following steps:
[0028] S1. Cultivation of Scenedesmus ellipsoides: use Scenedesmus ellipsoides as raw material, inoculate it into the culture medium at an inoculation density of 3 million cells / mL, and irradiate it with red and blue LED supplementary light for 16 hours / day, and the red light in the LED supplementary light The ratio with blue light is 1:1, the light intensity is controlled at 2000LX, the culture temperature is controlled at 23°C, cultured for 10 days, the density of Scenedesmus ellipsoides reaches 80 million cells / mL, and the cells of Scenedesmus ellipsoides are collected;
[0029] S2. Grease extraction: After concentrating and drying the Scenedesmus ellipsoidal cells collected in ...
Embodiment 2
[0034] Preparation of Scenedesmus ellipsoides culture medium: glucose 20mg / L, NaNO 3 2.3mg / L, KH 2 PO 4 1.8mg / L, chitosan 0.8mg / L, biogas slurry 8mg / L, f2 vitamin 3mL and seawater 1L;
[0035] A method for producing biodiesel with microalgae oil as raw material, comprising the following steps:
[0036]S1. Cultivation of Scenedesmus ellipsoides: use Scenedesmus ellipsoides as raw material, inoculate it into the culture medium at an inoculation density of 3 million cells / mL, and irradiate it with red and blue LED supplementary light for 14h / day, and the red light in the LED supplementary light The ratio with blue light is 1:2, the light intensity is controlled at 2500LX, the culture temperature is controlled at 24°C, cultured for 9 days, the density of Scenedesmus ellipsoides reaches 80 million cells / mL, and the cells of Scenedesmus ellipsoides are collected;
[0037] S2. Grease extraction: After concentrating and drying the Scenedesmus ellipsoidal cells collected in step S1...
Embodiment 3
[0042] Preparation of Scenedesmus ellipsoides culture medium: glucose 25mg / L, NaNO 3 2.2mg / L, KH 2 PO 4 2.0g / L, chitosan 0.6mg / L, biogas slurry 10mg / L, f2 vitamin 2mL and seawater 1L;
[0043] A method for producing biodiesel with microalgae oil as raw material, comprising the following steps:
[0044] S1. Cultivation of Scenedesmus ellipsoides: use Scenedesmus ellipsoides as raw material, inoculate into the culture medium at an inoculation density of 3 million cells / mL, and irradiate with red and blue LED supplementary light for 10h / day, and the red light in the LED supplementary light The ratio with blue light is 1:3, the light intensity is controlled at 3000LX, the culture temperature is controlled at 25°C, cultured for 10 days, the density of Scenedesmus ellipsoides reaches 80 million cells / mL, and the cells of Scenedesmus ellipsoides are collected;
[0045] S2. Grease extraction: After concentrating and drying the Scenedesmus ellipsoidal cells collected in step S1, th...
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