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Strain of Arthrobacter sp. B2 and application thereof to nitrogen-containing sewage degradation

A technology of Arthrobacter and sewage, applied in biological water/sewage treatment, water/sludge/sewage treatment, bacteria, etc., can solve the problems of long nitrogen treatment process, large aeration tank, high investment and operating costs

Active Publication Date: 2017-02-15
朗境环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The nitrogen treatment process is long, the HRT of the system is long, a large aeration tank is required, and the investment and operation costs are high; to maintain a high biological concentration and a good denitrification effect, sludge and nitrification liquid must be returned at the same time, etc.

Method used

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  • Strain of Arthrobacter sp. B2 and application thereof to nitrogen-containing sewage degradation
  • Strain of Arthrobacter sp. B2 and application thereof to nitrogen-containing sewage degradation
  • Strain of Arthrobacter sp. B2 and application thereof to nitrogen-containing sewage degradation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Screening and Identification of Example 1 Bacterial Strain B2

[0022] (1) Screening of strain B2

[0023] The activated sludge taken from the aeration tank of a sewage treatment plant in Lishui, Zhejiang was used as a sample, and the heterotrophic method was used to screen aerobic denitrifying bacteria (specific reference: Screening of efficient aerobic denitrifying bacteria and research on denitrification characteristics , Cai Changfeng, Liang Lei. Environmental Science and Technology, 2011,34(1):41-44.), isolated strain B2; its colony morphology is: colony milky white, opaque, colony surface is wet, the middle is raised, and the edge of the colony is smooth .

[0024] (2) Identification of strain B2

[0025] The genome of strain B2 was extracted, and the 16sRNA was determined (Reference: Isolation and identification of strain oligotrophic aerobic denitrifying bacteria and its denitrification characteristics, Wei Wei, Huang Tinglin, Su Junfeng, etc.; Journal of Ecol...

Embodiment 2

[0035] (1) Bacteria preparation

[0036] Arthrobacter B2 was inoculated into LB solid medium, and cultured at 37°C for 26 hours to obtain a bacterial slant; the final concentration of the LB solid medium was composed of: tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, agar 20g / L, solvent is water, pH 7.0.

[0037] Pick a ring of bacteria from the slant of the bacteria and inoculate it into LB liquid medium, and cultivate it at 37 °C for 28 hours to form a seed solution with a concentration of 1.6×10 8 cfu / mL. The final concentration of LB liquid medium is composed of: tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, solvent is water, pH 7.0.

[0038] The seed liquid is transferred to the 30L seed tank that fermented medium is housed with the inoculum amount of 3% (volume ratio) and carries out fermentation culture, and liquid filling capacity is 70%, and fermentation temperature is 37 ± 1 ℃, and stirring rate is 200rpm, and ventilation rate is 0.9-1.1V...

Embodiment 3

[0043] Take 20L of domestic sewage from the water inlet of a sewage plant, and put it in the simulated aeration tank in the laboratory, with a ventilation volume of 1m 3 / h; the inoculum dose is 30g; the treatment time is 24h. The sewage before treatment, the sewage after 24h treatment with bacterial agent, and the sewage after 24h treatment without bacterial agent (i.e. control) were measured by potassium dichromate method for COD, and TN (total nitrogen) was determined by potassium persulfate oxidation / ultraviolet spectrophotometry Determination by method; NH 3 -N (ammonia nitrogen) was determined by Nessler's reagent photometry. The results are shown in Table 3.

[0044] Table 3 Sewage Treatment Effect of Bacteria

[0045]

[0046] Remarks: domestic sewage - refers to the biochemical indicators of domestic sewage before treatment; bacterial agent - refers to the biochemical indicators of domestic sewage treated with bacterial agents; control - refers to domestic sewag...

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Abstract

The invention provides a strain of Arthrobacter sp. B2 and application thereof to nitrogen-containing sewage degradation. The Arthrobacter sp. B2 is preserved in CCTCC (China Center for Type Culture Collection) on September 14, 2016, and has the preservation number of CCTCC NO:2016485. The Arthrobacter sp. B2 has high denitrification capability under the aerobic condition; the nitratlon reaction and the denitrification reaction are realized; the organic matters in the water body and organic matters of ammonia nitrogen, nitrite nitrogen and the like can be effectively degraded in the sewage treatment oxidation pond; the water body purification treatment is realized. Therefore the prepared strain of the Arthrobacter sp. B2 is applied to sewage treatment and culture waste water purification industry. The degradation rate of the nitrate is 87.6 percent; the nitrite degradation rate is 84.5 percent.

Description

[0001] (1) Technical field [0002] The invention relates to a strain of heterotrophic-aerobic denitrifying bacteria, in particular to an Arthrobacter B2 and its application in sewage treatment. [0003] (2) Background technology [0004] Biological denitrification is the most economical and effective method to remove nitrogen pollution in sewage treatment. The traditional nitrogen removal process is realized through two processes with different conditions: nitrification and denitrification. In the aerobic aeration area, through the action of nitrifying bacteria, the ammonia nitrogen is oxidized and converted into nitrate nitrogen; in the anaerobic area, through the action of denitrifying bacteria, most of the nitrate is reduced and converted into nitrous oxide and nitrogen, and also A small part is converted into the nutrients of the bacteria themselves, so as to achieve the purpose of denitrification. The nitrogen treatment process is long, the HRT of the system is long, a ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/06C02F101/16
CPCC02F3/34C02F2101/16C12N1/20C12N1/205C12R2001/06
Inventor 周煕城张正波王雪玲蔡畅
Owner 朗境环保科技有限公司
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