Remediation methods of phthalate-contaminated soil
A phthalate, polluted soil technology, applied in the field of ecological environmental protection, to achieve the effect of large biomass, well-developed root system, and strong adaptability
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Embodiment 1
[0031] Embodiment 1: the degradation of Trichoderma viridans F7 terephthalates
[0032] Purified and cultivated Trichoderma viridans F7 was picked from the inoculation loop, inserted into 5g / L glucose solution, and incubated for 36 hours at 28°C in a constant temperature shaking incubator at 180r / min to induce spore germination and hyphal growth. Measure the OD value of the bacterial solution at 660nm with a spectrophotometer, and dilute it appropriately to make the concentration consistent (OD660≈0.8) to obtain the bacterial solution.
[0033] Prepare basal salt medium: MgSO 4 ·7H 2 O 0.5g, KCl 0.5g, K 2 HPO 4 1.31g, NaNO 3 3.0g, FeSO 4 ·7H 2 O0.018g, add distilled water to 1000mL; pH 7.0, sterilize at 121°C, 0.1MPa for 20min. Add PAEs to the sterilized basic salt medium to obtain PAEs medium. Four different concentration gradients were set up, the concentrations of the three PAEs were 0, 25, 50 and 100 mg / L, and the total concentrations of PAEs were 0, 75, 150 and ...
Embodiment 2
[0036] Embodiment 2: Trichoderma viridans F7 fermented liquid unites mung bean to remediate phthalate-contaminated soil
[0037] The test soil was taken from the farmland plow layer of Jiaoxi Town, Jiaozhou City, Qingdao, Shandong Province. The soil pH was 6.58 (soil-water ratio 1:2.5), the organic matter was 23.1g / kg, the cation exchange capacity was 13.2cmol / kg, and the total nitrogen content was 1.25g / kg. The contents of available phosphorus and potassium were 36.4mg / kg and 197mg / kg respectively. The obtained soil was air-dried, crushed and passed through a 2 mm (10 mesh) sieve.
[0038] The above soil was further crushed through a 0.3mm sieve. Take a small amount of soil that has passed through a 0.3mm sieve, add 5g / L PAEs stock solution, so that the concentrations of the three PAEs are all 1000mg / kg (the total concentration is 3000mg / kg), and mix with uncontaminated soil after the solvent evaporates to make three kinds of PAEs The concentration of PAEs is 50mg / kg (the t...
Embodiment 3
[0051] Example 3 Trichoderma viridans F7 solid preparation combined with mung bean remediation of phthalate-contaminated soil
[0052] The source of the soil used, its properties and basic types, the way of adding PAEs pollutants, and mung bean seeds are the same as in Example 2. The concentrations of the three PAEs in the artificially polluted soil used in the experiment were all 100mg / kg (the total concentration was 300mg / kg).
[0053] Preparation of Trichoderma viridans F7 solid preparation: inoculate the activated Trichoderma viridans F7 bacterium block into a Kelvin flask containing a slope of potato dextrose agar medium (PDA), culture at a constant temperature for 5 days at 28°C in the dark, then rinse with sterile water Wash the conidia and dilute the spore solution to 1×10 5 spores / mL as the seed solution for fermentation inoculation. Wheat bran was used as the medium for the propagation of Trichoderma viridans F7. After the wheat bran was washed, dried, and pulveriz...
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