Unlock instant, AI-driven research and patent intelligence for your innovation.
Phenylpropanoid compound and preparation method and application thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A kind of technology of phenylpropanoid and compound, applied in phenylpropanoid compound and preparation field thereof
Inactive Publication Date: 2017-02-22
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
View PDF0 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0006] Acorus tatarinowii is a plant of the genusCalamus in the family Araceae, which has the effects of removing dampness and harmonizing the stomach, resuscitating phlegm, refreshing the mind and improving intelligence. The reported biological activities of Acorus tatarinowii include (1) acting on the nervous system, such as antidepressant, sedative and Convulsions, brain protection and intelligence development, (2) acting on the cardiovascular system, such as lowering fat and slowing down the heart rate, (3) acting on the respiratory system, such as treating chronic bronchitis and bronchial asthma, (4) inhibiting bacteria, (5) ) anti-cancer, etc.; the chemical components of Acoruscalamus include volatile oil, lignans, phenolic acids, alkaloids, flavonoids, etc., and in terms of active material basis, there are many reports on volatile components, and few reports on non-volatile components
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
[0042] Herbal decoction pieces of Shichangpu were purchased from Anhui Jiren Pharmaceutical Co., Ltd. (batch number: 120519, specification: 1kg / bag, origin: Anhui), about 20kg, decocted 3 times with 8, 6, and 6 times the amount of water, 1.5h each time , combined extracts, recovered under reduced pressure to a small volume (80L), filtered through D101 macroporous resincolumn chromatography, ethanol-water (v / v) gradient elution, recovered solvent under reduced pressure, obtained 30%, 50%, 70%, 95% 4 ethanol eluates.
[0043] The 30% ethanol eluate was dispersed in water and adjusted to pH=3-4 with formic acid, extracted three times with an equal volume of n-butanol, and the n-butanol extract was recovered under reduced pressure to obtain a dry extract, which was dissolved in water and subjected to ODS column chromatography (methanol-water gradient elution), obtain 6 components, wher...
Embodiment 2
[0056] The influence of phenylpropanoid compound of the present invention on 5-hydroxytryptamine transporter (SERT)
[0057] Using stably transfected hSERT-HEK293 cell line, 4-(4-(dimethylamino)phenyl)-1-methylpyridinium (APP + ) is a fluorescent substrate, and the effect of phenylpropanoids on SERT activity is detected on a high-content system.
[0058] 1) Experimental instruments and reagents
[0059] laboratory apparatus:
[0060] High-content Operetta system and Columbus data management and analysis system (PerkinElmer), ultra-clean bench, pipette gun (1000μL, 200μL, 20μL, 10μL, 2.5μL, Eppendorf, USA)
[0061] Reagents and materials:
[0062] Human embryonic kidneycell line HEK293 (CellBank of Type Culture Collection Committee, Chinese Academy of Sciences), hSERTpcDNA3 plasmid (Addgene, plasmid 15483), MEM medium (Gibco), APP+ (Sigma), Hoechst 33342 (Cell Signaling Technology), 96-well plate (Costar 3605)
[0063] 2) Experimental operation process
[0064]First est...
Embodiment 3
[0084]
[0085]
[0086] Preparation method: uniformly mix phenylpropanoid compound, lactose and starch according to the above ratio, pass through a 200-mesh sieve, moisten evenly with water, dry the wetted mixture and sieve, add magnesiumstearate, and press the mixture Tablets, each weighing 250mg, with an active ingredient content of 10mg.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention provides a phenylpropanoid compound in acoruscalamus and a preparation method and application thereof. The compound is obtained through separation and purification of (R)-1,2-split-ring ceplignan, (2S,3R)-ceplignan and (2R,3S)-ceplignan from acorus gramineus rhizomes, has 5-hydroxytryptamine transporter (SERT) adjusting activity, can be applied to preparation of drugs for resisting depression, anxiety disorder, schizophrenia, obsessive-compulsive disorder neurodegeneration and other diseases and preparation of drugs for treating drugaddiction, digestive system dysfunction and other diseases, and has important drug development value.
Description
technical field [0001] The invention relates to the field of traditional Chinese medicine research, in particular to phenylpropanoid compounds and their preparation methods and applications. Background technique [0002] Serotonintransporter (SERT) is a transmembrane transporter with high affinity for 5-HT, containing about 630 amino acid residues, and its coding gene (SLC6A4) is located on chromosome 7 and 11, respectively. It consists of 14 exons of about 35kb. The SERT protein contains 12-13 transmembrane regions, the N-terminal and C-terminal are located in the cytoplasm, there is a cAMP-dependent proteinkinasebinding site near the N-terminal, and there is an extracellular region between the third and fourth transmembrane regions. The cyclic portion of is the N-linked glycosylation site. [0003] SERT belongs to Na + / Cl - Dependent transporter, mainly located in 5-HTergic neurons. SERT re-uptakes 5-HT from the synaptic cleft into presynaptic neurons, directly af...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More