Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of trichoderma protoplast fusion strain and application thereof

A technology of protoplast fusion and Trichoderma, applied in application, biocide, microorganisms, etc., can solve the problems of unsatisfactory field effects of Trichoderma preparations and restrictions on popularization and application, and achieve excellent biocontrol properties, high control effect, and stress resistance strong effect

Active Publication Date: 2019-09-24
山东济清科技服务有限公司
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the unsatisfactory field effect of Trichoderma preparations from natural sources, its further application is restricted.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of trichoderma protoplast fusion strain and application thereof
  • A kind of trichoderma protoplast fusion strain and application thereof
  • A kind of trichoderma protoplast fusion strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1 Trichoderma fusion son Tpf-2

[0021] The preservation number of the Trichoderma fusion son tpf-2 of the present invention is CGMCC No.12477, the preservation date is May 30, 2016, the classification name is Trichoderma sp., and the reference microbial strain is Tpf-2. The preservation unit is the General Microbiology Center of the China Committee for the Collection of Microbial Cultures, and the address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The invention is formed by fusion of Trichoderma harzianum T. harzianum TW21990 and Trichoderma paracyanodichotomus TW21990-1 through protoplast fusion.

Embodiment 2

[0022] Embodiment 2 protoplast fusion

[0023] The parental strains used in the experiment were T.harzianum TW21990 and T.cyanodichotomus TW21990-1. After the young mycelia of the two kinds of Trichoderma were treated with a pretreatment agent, the mycelium of TW21990 was lysed with Lysing Enzyme, and the mycelium of TW21990-1 was lysed with Driselase. Filter with a layer of lens paper to remove residual mycelia, collect protoplasts by centrifugation and wash with STC, and adjust the protoplast volume by 10 6 individual / mL.

[0024] The biological strain preservation number of TW21990 is: CGMCC No.12864, and the preservation date is: September 1, 2016. The preservation unit is the General Microbiology Center of the China Microbiological Culture Collection Management Committee, and the address is No. 1 Beichen West Road, Chaoyang District, Beijing Hospital No. 3, the taxonomic name is Trichoderma harzianum.

[0025] The biological strain preservation number of TW21990-1 is: ...

Embodiment 3

[0035] Embodiment 3 Fusion screening

[0036] Determination of the biocontrol properties of the fusion, mainly including the inhibition of Pythium melon and Pythium ultima on the PDA plate; the width of the hydrolysis band produced on the enzyme activity plate, including β-1,3-glucanase, Cellulase and protease; yield of chlamydospores in liquid fermentation. The gray correlation degree analysis method was used to comprehensively evaluate the fusions obtained from the preliminary screening, and the excellent fusions were determined according to the correlation degree.

[0037] Inhibition rate%=100%×(control bacteria growth radius-treatment bacteria growth radius) / control bacteria growth radius

[0038] 50×Trace elements (L): FeS0 4 ·7H 2 0 0.25g, ZnSO 4 2H 2 O 0.07g, CoCl 2 ·6H 2 O 0.1g, MnSO 4 ·H 2 O 0.085g.

[0039] β-1,3-glucanase medium (L): glucose 20g, yeast extract 0.67g, K 2 HPO 4 15g, (NH 4 ) 2 S0 4 5g, CaCl 2 0.6g, MgCl 2 0.6g, 50×trace elements 20...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a Trichoderma protoplast fusion strain and application thereof. The Trichoderma protoplast fusion strain is Trichoderma fusogen Tpf-2, and its preservation number is CGMCC No.12477, and the preservation date is May 30, 2016. The unit is the General Microbiology Center of the China Committee for the Collection of Microbial Cultures, and the address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The present invention can effectively prevent and control the diseases caused by Pythium melons and fruits and Pythium ultima, and the control effects on tomato damping-off caused by Pythium melons and fruits and Pythium ultima respectively reach 86.3% and 85.7%, and the effects of increasing yield reach 100% respectively. 77.3% and 79.9% (p<0.01), the five-month survival rate of colonization in the tomato rhizosphere soil reached 75%, and the effective survival rate of the chlamydospore preparation reached 90% within the one-year shelf life.

Description

technical field [0001] The present invention relates to a Trichoderma protoplast fusion strain and its application, in particular to a Trichoderma fusion obtained by fusion of heat-ultraviolet inactivated protoplasts and the application of the fusion in the control of tomato Pythium damping-off, Belongs to the field of biotechnology. Background technique [0002] Tomato is the main vegetable crop in my country. Various diseases and insect pests often occur during the planting process. Among them, tomato damping-off is an important disease of tomato, which mostly occurs in the seedling stage and leads to the collapse of seedlings. Pythium melon and fruit P.aphanidermatum and Pythium ultimum P.ultimum, this type of pathogen is a lower fungus of the genus Pythium in the subphylum Flagellate of Oomycota, with a fast growth rate, often saprophytic or parasitic in soil and water Survive for a long time and bring serious losses to agricultural production. [0003] The bacterial age...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
CPCA01N63/30C12N1/145C12R2001/885
Inventor 陈凯李纪顺魏艳丽王贻莲扈进冬李玲赵忠娟杨合同
Owner 山东济清科技服务有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com