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Preparation and application of nano cerium oxide/mesoporous carbon sensor for detecting pesticide

A technology of nano-cerium oxide and mesoporous carbon, which is applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems of inapplicable on-site detection, time-consuming, and endangering human life

Inactive Publication Date: 2017-02-22
SHANDONG UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] my country is a large vegetable production and consumption country. In order to ensure the output of vegetables and increase the income of farmers, pesticides will be used in large quantities or even unreasonably in the process of planting vegetables, causing environmental pollution, frequent pesticide residues in vegetables, and even endangering human health. Life is dangerous. Most of the insecticides used in my country are organophosphorus pesticides, accounting for as much as 70%. The main reason is that organophosphorus pesticides are highly toxic and have a wide contact area. In addition to use, timely and accurate detection of organophosphorus pesticide residues in vegetables is an important part of ensuring vegetable safety, so it is necessary to find a fast and accurate detection method
[0004] Commonly used traditional pesticide residue detection methods include gas chromatography, high performance liquid chromatography, mass spectrometry, gas chromatography-mass spectrometry, etc. These detection methods are qualitative and quantitative, but the instruments used are expensive and have high requirements for the external environment, and It needs cumbersome pretreatment steps such as separation, extraction, and derivatization of the sample, which is time-consuming and costly, requires professional and technical personnel to maintain, and is not suitable for on-site detection; acetylcholinesterase based on screen-printed electrodes and nanomaterials such as cerium oxide The (AChE) biosensor measures the concentration of pesticides by detecting the change in the current generated by the enzyme-catalyzed substrate before and after exposure to pesticides. The use of nano-materials can amplify the detection signal. Screen-printed electrodes and nanomaterials are used in acetylcholinesterase biosensors, which make the detection equipment relatively convenient to carry, simple to operate, high in detection signal, and fast in analysis speed, which makes the prepared sensor more suitable for on-site rapid detection of pesticide residues

Method used

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  • Preparation and application of nano cerium oxide/mesoporous carbon sensor for detecting pesticide
  • Preparation and application of nano cerium oxide/mesoporous carbon sensor for detecting pesticide
  • Preparation and application of nano cerium oxide/mesoporous carbon sensor for detecting pesticide

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Example 1 Preparation and application of a sensor for detecting pesticides based on nano-cerium oxide / mesoporous carbon

[0040] 1) Preparation of nano-cerium oxide-chitosan composites and ordered mesoporous carbon-chitosan composites: weigh 0.5 g chitosan in a beaker, add 1.0% acetic acid solution and stir to dissolve, dissolve well The solution was placed in a 250 mL volumetric flask and constant volume, the solution after constant volume was poured into a beaker, and magnetically stirred under a magnetic stirrer for 10 h to obtain a 0.2% chitosan solution; take 4 mg of nano-cerium oxide and disperse it in In 4 mL of 0.2% chitosan solution, sonicate in an ultrasonic cleaner until uniformly dispersed to obtain a 1 mg / mL nano-cerium oxide-chitosan composite; take 2 mg of ordered mesoporous carbon and dissolve in 4 ml of 0.2 % chitosan solution, in an ultrasonic cleaner for 1 hour to obtain a uniform, black suspension, which is 0.5 mg / mL ordered mesoporous carbon-chitosa...

Embodiment 2

[0044] Example 2 Electrochemical characterization during electrode assembly

[0045] figure 1 Shown are the cyclic voltammetry curves of different electrodes in a phosphate buffer solution containing 0.1 mol / L KCl and 5 mmol / L potassium ferricyanide. Curve (a) in the figure is a characterization diagram of an empty screen-printed electrode, We can see the obvious redox peak; as shown in the curve (b) in the figure, when the screen printing electrode is decorated with cerium oxide-chitosan nanomaterials, the current increases significantly; as shown in the curve (d) After modifying the ordered mesoporous carbon-chitosan material on this basis, the current increased significantly because the ordered mesoporous carbon also had good conductivity; when 5 μL of 0.02 U of acetylcholinesterase was immobilized, Since the enzyme is a macromolecular protein, it not only does not conduct electricity, but also hinders the electron transfer at the interface, so the peak value of the curren...

Embodiment 3

[0047] Example 3 Optimization of test conditions

[0048] Three test conditions of acetylcholinesterase biosensor The pH value of the test bottom solution, the amount of acetylcholinesterase immobilized, and the incubation time were optimized through the quadratic rotation orthogonal test, and the best test conditions were obtained: pH value 8.0, acetylcholinesterase immobilized amount 0.1 U, incubation time 12 min; prepare phosphate buffers with pH values ​​of 7.0, 7.4, 8.0, 8.6, and 9.0, and use these phosphate buffers to prepare a series of pH values ​​of 1.0 mmol / L thiochloride Acetylcholine base solution, the fixed amount of acetylcholinesterase was 0.06 U, 0.076 U, 0.1 U, 0.124 U, 0.14 U; the pesticide inhibition time was controlled as 8 min, 9.6 min, 12 min, 14.4 min, 16 min, according to the designed According to the test plan, the current value was detected, and the optimal test conditions were selected: pH value 8.0, acetylcholinesterase immobilization amount 0.1 U, ...

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Abstract

The invention discloses preparation and application of a nano cerium oxide / mesoporous carbon sensor for detecting a pesticide, and belongs to the technical field of safe and quick detection on the quality of agricultural products. According to the preparation and the application of the nano cerium oxide / mesoporous carbon sensor for detecting the pesticide, the surface of a cleaned and activated screen-printed electrode is successively modified by a prepared nano cerium oxide-chitosan compound and a prepared ordered mesoporous carbon-chitosan compound; afterwards, acetylcholin esterase is dropwise added on the well modified electrode; the pesticide is detected through calculating the change of a current before and after the sensor is in contact with the pesticide; the concentration of pesticide residues in a fruit and a vegetable are quantified according to a standard curve. In an experiment, a quadratic rotation-orthogonal experiment is adopted, experimental parameters are optimized, so as to obtain optimized parameters that the pH (potential of Hydrogen) value of a testing base solution is 8.0, the fixed quantity of the acetylcholin esterase is 0.1U, and the inhibition time of the pesticide is 12min. The prepared acetylcholin esterase sensor is low in detection limit, good in stability and quick in detection, is applicable to the detection on organic phosphorus type and carbamate type pesticides, and can be used for detecting pesticide residue in a practical sample.

Description

technical field [0001] The invention relates to the preparation and application of a sensor for detecting pesticides based on nano-cerium oxide / mesoporous carbon, and belongs to the technical field of rapid detection of agricultural product quality and safety. [0002] technical background [0003] my country is a large vegetable production and consumption country. In order to ensure the output of vegetables and increase the income of farmers, pesticides will be used in large quantities or even unreasonably in the process of planting vegetables, causing environmental pollution, frequent pesticide residues in vegetables, and even endangering human health. Life is dangerous. Most of the insecticides used in my country are organophosphorus pesticides, accounting for as much as 70%. The main reason is that organophosphorus pesticides are highly toxic and have a wide contact area. In addition to use, timely and accurate detection of organophosphorus pesticide residues in vegetables ...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/327
Inventor 孙霞张前前郭业民
Owner SHANDONG UNIV OF TECH
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