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Integrated nanoprobe for diagnosis and treatment of bacterial infection in vivo and preparation method thereof

A nanoprobe and bacterial infection technology, which is applied in the field of medical nanomaterials, can solve the problems of poor accuracy, narrow application area, and low detection sensitivity, and achieve high-sensitivity diagnosis, good biocompatibility, and high detection sensitivity.

Active Publication Date: 2019-09-03
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these probes have disadvantages such as low detection sensitivity, poor accuracy, and narrow application

Method used

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  • Integrated nanoprobe for diagnosis and treatment of bacterial infection in vivo and preparation method thereof
  • Integrated nanoprobe for diagnosis and treatment of bacterial infection in vivo and preparation method thereof
  • Integrated nanoprobe for diagnosis and treatment of bacterial infection in vivo and preparation method thereof

Examples

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Effect test

Embodiment 1

[0041] (1) Preparation of silicon nanoparticles: Take 40 mL of absolute ethanol, add 10 mL of water, mix well, then add 900 μL of ammonia water (mass fraction 28-30%), stir for 5 minutes, and then add 600 μL of TEOS (orthosilicon ethyl acetate), and after stirring vigorously for 1 h, 600 μL TEOS was added again, and stirring was continued for 24 h. Finally, the reaction solution was centrifuged at 9500 r / min for 25 min, washed three times with water and ethanol, and finally dispersed in water to obtain solid silicon nanoparticles.

[0042] (2) Preparation of polyelectrolyte-wrapped silicon nanoparticles: Take 100mg of PAH (polypropylene ammonium chloride, Mw=15000g / mol), dissolve it in 10mL of 1mM NaCl aqueous solution, and then add 1mL of step (1) The synthesized silicon nanoparticles were stirred and reacted for 3h. Finally, the reaction solution was centrifuged at 9500 r / min for 25 min, washed with water three times, and finally dispersed in water to obtain silicon nanopar...

Embodiment 2

[0053] (1) Preparation of silicon nanoparticles: Take 11.4mL absolute ethanol, add 72mL water, mix well, then add 2.9gCTAB (cetyltrimethylammonium bromide) and 625μL ammonia water (mass fraction is 28-30% ), stirred at 60°C for 30min, then added 7.3mL TEOS (tetraethyl orthosilicate) under stirring condition, and stirred vigorously at 60°C for 2h. Finally, the reaction solution was centrifuged at 9500r / min for 25min, washed three times with water and ethanol, and finally dispersed in water to obtain mesoporous silicon nanoparticles, which were calcined at high temperature to remove CTAB and dispersed in water for later use.

[0054] (2) Preparation of polyelectrolyte-wrapped silicon nanoparticles: Take 100mg of PEI (polyethyleneimmonium, Mw=20000g / mol), dissolve it in 10mL of 1mM NaCl aqueous solution, and then add 1mL of step (1) to synthesize Silicon nanoparticles were stirred vigorously for 3 h. Finally, the reaction solution was centrifuged at 9500 r / min for 25 min, washed...

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Abstract

The invention relates to a diagnosis and treatment integrated nano-probe for in vivo bacterial infection. The nano-probe comprises silicon nanoparticles wrapped by a polycation electrolyte layer, wherein the outer side of the polycation electrolyte layer is modified with near-infrared organic fluorescent dye, and the polycation electrolyte layer is cross-linked with polyethylene glycol and bacterial targeting molecules. The invention further discloses a preparation method of the diagnosis and treatment integrated nano-probe. The method comprises steps as follows: preparing the silicon nanoparticles with particle size of 60-80 nm; adding the silicon nanoparticles to a salt solution of the polycation electrolyte, and stirring the mixture for a reaction to obtain the polycation electrolyte wrapped silicon nanoparticles; dissolving the near-infrared organic fluorescent dye in an organic solvent, adding a covalent coupling agent for activation, and then adding the polycation electrolyte wrapped silicon nanoparticles for a reaction to obtain the silicon nanoparticles with surfaces modified with the near-infrared organic fluorescent dye; activating polyethylene glycol and the bacterial targeting molecules, and crosslinking the activated polyethylene glycol and bacterial targeting molecules to the polycation electrolyte on the surfaces of the silicon nanoparticles to obtain the diagnosis and treatment integrated nano-probe for in vivo bacterial infection.

Description

technical field [0001] The invention relates to the field of medical nanomaterials, in particular to an integrated nanoprobe for diagnosis and treatment of bacterial infection in vivo and a preparation method thereof. Background technique [0002] Bacteria are an important class of pathogens. After they invade the body, they will secrete a large amount of biological toxins, thereby destroying the structure and function of the body and causing host infection. In recent years, due to the abuse of antibiotics, a large number of drug-resistant bacteria (such as methicillin-resistant Staphylococcus aureus MRSA) have emerged. Infectious diseases caused by pathogenic bacteria have become important killers that endanger human health, and have attracted widespread global attention. Therefore, in order to carry out the early diagnosis and treatment of bacterial infection diseases, it is of great significance to study the rapid detection and treatment technology of pathogenic bacteria....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00A61K41/00A61K47/04A61K47/32A61K47/10A61K47/42C09K11/06
Inventor 李永强赵志伟严荣
Owner SUZHOU UNIV
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