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Organic acid preparation method

A technology of organic acid and glycolic acid, which is applied in the preparation of organic compounds, organic chemical methods, carboxylate preparation, etc., can solve the problems of low yield and cannot be prepared with glycolic acid, and achieve high practicability

Inactive Publication Date: 2017-03-08
HENGYANG YISHUN CHEM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Like this, because the yield of this preparation method is very low, this preparation method cannot be used in the preparation of the glycolic acid on the industry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: (Acquisition of 2-keto-D-gluconic acid non-producing bacteria and 5-keto-D-gluconic acid non-consuming mutant strains)

[0028] In order to prepare non-2-keto-D-gluconic acid-producing strains derived from Gluconobacter oxydans NBRC 3172 strains, NTG mutation treatment was performed on NBRC 3172 strains, and screening of 2-keto-D-gluconic acid non-producing bacteria was attempted. Specifically, it was carried out by the following method.

[0029]Gluconobacter oxydans NBRC 3172 strain grown in MA agar medium [2.5% mannitol, 0.5% yeast extract, 0.2% peptone, 1.5% agar (no pH adjustment)] was inoculated with 1 platinum circle into the After autoclaving 30mL of MA medium at 121°C for 20 minutes in a 300mL Erlenmeyer flask with a capacity of 300mL, shake culture at 220 rpm per minute at 30°C for 19 hours, and aseptically obtain cells from the culture solution by centrifugation . The obtained cells were washed once with 0.85% sterilized physiological saline, and ...

Embodiment 2

[0031] Embodiment 2: (preparation of tartaric acid and glycolic acid (adding transition metal catalyst) by glucose)

[0032] The Gluconobacter oxidans TADK-267 strain obtained in the above Example 1 was inoculated into a medium containing 5 mL of MB medium [2.5% mannitol, 0.5 yeast extract, 0.3% peptone] heat-sterilized at 121°C for 20 minutes. Incubate the 3 test tubes at 28°C with reciprocating shaking at 250 rpm for 17 hours. Combine the culture solutions in these 3 test tubes as the seed culture solution. The culture is carried out as follows. In a 1L fermenter, add 6% glucose, 0.09% ammonium chloride, 0.06% potassium dihydrogen phosphate, 0.18% corn steep liquor powder, 0.1% yeast extract, 0.015% magnesium sulfate 7 hydrate salt , 0.0029% manganese sulfate pentahydrate, and 0.12% calcium chloride, 500 mL of the culture medium was heat-sterilized in the same manner, and 10 mL of the above-mentioned seed culture solution was inoculated to start this culture. While adjusti...

Embodiment 3

[0033] Embodiment 3: (preparation of tartaric acid and glycolic acid (add palladium carbon) by glucose)

[0034] After culturing Gluconobacter oxydans TADK-267 in a fermenter for 50 hours in the same manner as in Example 2 above, the glucose was completely consumed and 2.8 g / L of gluconic acid remained, but 67.4 g / L of 5-keto- D-gluconic acid. After adding 6M potassium hydroxide solution to the culture solution to raise the pH of the culture solution to 9.6, add 10 g of palladium activated carbon (10%), and then add potassium hydroxide solution to maintain the pH above 9.6 while continuing to aerate After stirring until the 144th hour, a reaction liquid containing 31.9 g / L of tartaric acid and 15.9 g / L of glycolic acid was obtained. If considering the change of the liquid volume caused by evaporation and adding potassium hydroxide solution, and the liquid volume reduction caused by sampling in the middle to calculate, the molar yield of tartaric acid of glucose is 61%, and th...

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Abstract

The present invention relates to an organic acid preparation method, which comprises steps A-C, wherein L-tartaric acid or a salt thereof, and / or glycolic acid or a salt thereof is prepared from glucose. The method specifically comprises: (A) culturing microorganisms capable of producing 5-keto-D-gluconic acid through glucose by using a glucose-containing culture liquid in the presence of an alkali capable of making the 5-keto-D-gluconic acid form a water-soluble salt to obtain a culture liquid containing the 5-keto-D-gluconic acid water-soluble salt; (B) adjusting and maintaining the pH value of the culture liquid containing the 5-keto-D-gluconic acid water-soluble salt at 7-12 to obtain a reaction liquid capable of converting the 5-keto-D-gluconic acid water-soluble salt into L-tartaric acid or a salt thereof, and / or glycolic acid or a salt thereof; and (C) extracting the L-tartaric acid or the salt thereof, and / or the glycolic acid or the salt thereof through the reaction liquid obtained in the step (B). According to the present invention, the method is effect, has the low cost, can be used in the industrial preparation, and has the extremely high practicability.

Description

technical field [0001] The invention relates to the field of organic acids, in particular to a method for preparing organic acids. Background technique [0002] At present, L-tartaric acid or its salt is used as sour agent and pH regulator in the food field, as cosmetics, dyeing, lotion, and plating in the industrial field, and as pharmaceutical raw materials in the pharmaceutical field. useful substance widely used. In addition, when L-tartaric acid is produced from glucose, glycolic acid produced simultaneously with L-tartaric acid is useful as a metal detergent, a plating additive, a cosmetic additive, a raw material for hard biodegradable polymers, and the like. Various methods are known for producing L-tartaric acid, but it is currently produced from winemaking residues and petroleum-derived intermediate maleic anhydride. Furthermore, glycolic acid is produced by petrochemical methods. On the other hand, the method of producing tartaric acid and glycolic acid via 5-k...

Claims

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Application Information

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IPC IPC(8): C12P7/46C12P7/42C07C59/255C07C59/06C07C51/353C07C51/00
CPCC12P7/46C07B2200/07C07C51/00C07C51/353C12P7/42C07C59/255C07C59/06
Inventor 罗秋
Owner HENGYANG YISHUN CHEM CO LTD
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