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A kind of carbon quantum dot nano material and its preparation method and application

A nanomaterial and carbon quantum dot technology, applied in the field of organic carbon quantum dot synthesis, can solve problems such as limiting the application of PEI, and achieve the effect of simple and efficient synthesis method, favorable for cell wall diffusion, and uniform particle size

Active Publication Date: 2019-07-23
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the existence of these shortcomings, the application of PEI is limited

Method used

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  • A kind of carbon quantum dot nano material and its preparation method and application
  • A kind of carbon quantum dot nano material and its preparation method and application
  • A kind of carbon quantum dot nano material and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Dissolve 0.15 g of citric acid, 0.13 g of mercaptoethylamine, and 0.1 g of polyethyleneimine (Mw = 800) in 3 mL of ultrapure water, stir well, and ultrasonically (power 200 W, frequency 28 KHz) for 3 min at 170 ℃, heated with a single-mode microwave synthesizer (power 500W) for 10 min, dialyzed the synthesized mixture through a dialysis bag (Mwco: 1000) in ultra-pure water at 45 °C for 36 h, changed the ultra-pure water every 10 h, and rotated in vacuum Collect the material after drying in a desiccator, and detect the fluorescence emission with a UV lamp (305 nm, 365 nm), such as figure 1 As shown, the synthesized material is transparent brown-yellow under white light, and has high-brightness fluorescence emission under ultraviolet light (305 nm, 365 nm). Malvern particle size analyzer detects the DLS particle size and Zeta potential value of the synthetic material. The particle size is about 0.7nm, which is relatively small; it is positively charged, about +8.18 mV, so...

Embodiment 2

[0041] Disperse 20 μL of the material synthesized in Example 1 in the water system, add 10 μg of the plasmid into ultrapure water, make up the total amount to 50 μL, and ultrasonically shake in an ice bath for 3 minutes in an ultrasonic cleaner, and then put it in a dark place to combine After 30 min, add it to the single cells of the Liriodendron suspension system sieved through a 300-mesh cell sieve, mix well, then transfer to the cell culture plate, add 1 mL of ultrapure water, and observe the fluorescent carbon dots in the Liriodendron suspension system after 24 hours. distribution in cells. It can be seen from Example 1 that the synthetic material has certain positively charged groups, which can adsorb plasmids through electrostatic interaction, and co-incubating with cells can adsorb cell walls, such as Figure 12 As shown, the GFP-adsorbed green fluorescent protein expression plasmid can be delivered into the cell for expression, so that there is a high-brightness green...

Embodiment 3

[0043] Using different ratios of reaction substrates, the materials were synthesized by a microwave synthesizer at different reaction temperatures for 10 minutes. 0.15 g citric acid, 0.08 g mercaptoethylamine, and 0.2 g polyethyleneimine (Mw = 800) were dissolved in 3 mL of ultrapure water, stirred and mixed, ultrasonicated for 3 min, heated at 150 °C for 10 min in a microwave synthesizer, and the synthesized mixture was dialyzed in ultrapure water at 45 °C for 36 h through a dialysis bag (Mwco: 1000). The ultrapure water was changed every 10 h, and the material was collected after drying in a vacuum rotary dryer. 200 μL was taken and 1 mL of single cells of Liriodendron tulipifera suspension were co-incubated in 3 mL of ultrapure water for 24 h. Material-specific fluorescence found that plasmolysis appeared in the cells treated with the synthesized sample, and multiple endocytic vesicles with specific fluorescence distributed on the inner side of the cell membrane appeared in ...

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Abstract

The invention discloses a carbon quantum dot nanomaterial and its preparation method and application. The method is as follows: take citric acid, mercaptoethylamine, and polyethyleneimine, dissolve them in ultrapure water, stir and mix them evenly, and perform ultrasonic treatment on the 120-170°C, microwave heat treatment, dialyze the reaction-synthesized mixture in ultrapure water through a dialysis bag, and dry it with a vacuum rotary dryer after completion, and collect the material. In the present invention, under microwave-controlled auxiliary heating conditions, carbon quantum dots with multiple biological functions are hydrothermally synthesized by mercaptoethylamine-tuned citric acid and polyethyleneimine, and can obtain different material characterization properties such as high-brightness fluorescence or high charge. The carbon quantum dots can meet the needs of different biological applications. On the basis of changing the synthesis conditions, the fluorescent markers of the cell walls of living plant cells with high aqueous phase dispersibility and low cytotoxicity can be selected, or the cell walls of plant cells can be promoted. Materials that swallow and efficiently absorb external solutions, or materials that deliver nucleic acids and transfect intact plant cells with cell walls.

Description

technical field [0001] The invention belongs to the technical field of synthesis of organic carbon quantum dots with biological functions, and relates to a method for preparing fluorescent polymers or carbon dots synthesized by microwave controllable auxiliary heat to promote mercaptoethylamine-tuned citric acid and polyethyleneimine, in particular to a carbon dot Quantum dot nanomaterials and their preparation methods and applications. Background technique [0002] With the rapid development of genome high-throughput sequencing technology, researchers have accumulated genome sequences of many species, and the research on these species has also entered the era of functional genomes. Among them, many genes predicted by bioinformatics need to be studied for functional identification and functional interaction. Therefore, a variety of novel and precise gene function research technologies are emerging, such as gene editing technology, RNA interference, gene site-directed mutatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/65C01B32/15B82Y40/00B82Y30/00B82Y20/00C12N15/87G01N21/64
CPCC01P2002/80C01P2002/84C01P2004/64C09K11/65C12N15/87G01N21/6486
Inventor 施季森陈桢雨夏兵陈金慧霍爱玲王宾
Owner NANJING FORESTRY UNIV
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