Method for detecting glutathione concentration of solution

A technology of glutathione and concentration, which is applied in the direction of measuring devices, instruments, and material analysis through electromagnetic means, to achieve the effects of strong conductivity, simple preparation, and increased specific surface area

Active Publication Date: 2017-03-22
滁州市南方黑芝麻食品有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, there are many methods for determining glutathione content, such as enzyme cycle method, colorimetry, fluorescence photometry, flow cytometry, titration, high performance capillary electrophoresis and high pressure liquid chromatography devel

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Examples

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Example Embodiment

[0028] Example 1:

[0029] A method for detecting the concentration of glutathione in a solution. A three-electrode system is used to detect the glutathione in the solution to be tested by cyclic voltammetry. According to the linear equation of glutathione, the concentration of glutathione in the solution to be tested is obtained. Concentration, the working electrode in the three-electrode system is modified by aminated graphene quantum dots, and the working electrode is a glassy carbon electrode.

[0030] Wherein, in the method for detecting the concentration of glutathione in the solution, the specific operation of the modified treatment of aminated graphene quantum dots is as follows: immerse the working electrode in a solution containing 2 mg / mL aminated graphene quantum dots, and scan by cyclic voltammetry Take it out and place it in an infrared drying oven for drying. The scanning parameters are: initial potential 0V, highest potential 1V, lowest point 0V, final potential 0V,...

Example Embodiment

[0040] Example 2:

[0041] On the basis of Example 1, in the method for detecting the concentration of glutathione in the solution, the working electrode is pretreated and immersed in an electrolyte containing 10mM zirconium oxychloride and 0.3M potassium chloride with magnetized water. A layer of nano-zirconia is electrodeposited, and then modified with aminated graphene quantum dots. Finally, 10 μL of the dispersion liquid is dropped on the working electrode with the electrodeposited nano-zirconia layer. After drying, 7 μL of 0.03wt% Nafion Drop it on the surface of the electrode and dry it for later use. The dispersion is a mixture of fumed white carbon black, yeast glucan, propolis powder, and 20% acetic acid in a mass ratio of 15:1:1:500. It is prepared by ultrasonic dispersion, and the propolis powder is obtained by crushing the propolis through a 200-mesh sieve.

[0042] The method of Example 2 measured a better linear relationship between the current intensity and the glut...

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Abstract

The invention discloses a method for detecting the glutathione concentration of a solution. According to the method, a three electrode system is used, cyclic voltammetry is adopted to measure the glutathione concentration of a solution to be measured, the glutathione concentration of the solution can be obtained through a glutathione linear equation; in the three electrode system, the work electrode is subjected to an aminated graphene quantum dot modification treatment, and the work electrode is a glass-carbon electrode. The method has the advantages of simple operation, quick detection, and high sensitivity, and is capable of high-sensitively recognizing glutathione in a mixed sample solution.

Description

technical field [0001] The invention relates to the technical field of glutathione detection. More specifically, the present invention relates to a method for detecting the concentration of glutathione in a solution by using cyclic voltammetry based on the electrochemical sensing modified by aminated graphene quantum dots. Background technique [0002] Glutathione is a tripeptide containing γ-amide bonds and sulfhydryl groups. It is composed of glutamic acid, glycine and cysteine. It is widely found in animals and plants and plays an important role in the physiological activities of organisms. There are two forms of glutathione, reduced and oxidized, and reduced glutathione exists in large quantities in the body and plays a major role. Glutathione is usually referred to as reduced glutathione. The molecular formula of glutathione is C 10 h 17 N 3 o 6 S is a colorless, transparent, slender crystal with a melting point of 189-193°C. In the structure of glutathione, ther...

Claims

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Application Information

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IPC IPC(8): G01N27/48G01N27/327
CPCG01N27/3277G01N27/3278G01N27/48
Inventor 肖琦黄珊冯萌萌卢双燕黄初升苏炜何英姿
Owner 滁州市南方黑芝麻食品有限公司
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