Application of a hplc detection method for citric acid content in sugarcane leaves
A technology of sugarcane leaves and detection methods, which is applied to measurement devices, instruments, scientific instruments and other directions to achieve the effects of good repeatability and accuracy, fast detection speed and high purity
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Embodiment 1
[0026] The HPLC detection method of citric acid content in the sugarcane leaf of embodiment 1
[0027] S1. Select sugarcane leaves infected with the pathogen of sugarcane tip rot as the sample to be tested, take 2 g of the sample to be tested, add 4 mL of methanol water solution with a volume fraction of 80% pre-cooled to 4 ° C, add liquid nitrogen to grind into a slurry, and use ultra-clean Dilute water to 10ml, extract at 4°C for 1 hour, ultrasonically extract for 2 hours, centrifuge at 10,000r / min for 10 minutes, take the supernatant, add 2mL of the residue to 80% methanol aqueous solution pre-cooled to 4°C, and extract ultrasonically for 30 minutes, 10,000r / min Centrifuge for 10 min, take out the supernatant after centrifugation, combine the supernatant, and dilute to 8mL with a volume fraction of 80% methanol aqueous solution. In the bottle, as a sample solution for later use;
[0028] S2. Use HPLC to detect the content of citric acid in the sample solution.
[0029] S2...
Embodiment 2
[0042] Example 2 Judgment of the difference in susceptibility and resistance of sugarcane varieties infected with tip rot
[0043] (1) Preparation of inoculum: Inoculate the pathogenic bacteria of sugarcane tip rot on PDA medium for 3 days to activate, pick the mycelia from the edge of PDA medium and inoculate them in sterilized potato glucose water medium for 3 days, centrifuge, Use sterilized absorbent cotton to filter and collect the obtained bacteria, and use sterile water and bacteria to prepare a concentration of 1 × 10 6 CFU / ml sugarcane tip rot pathogen spore suspension.
[0044] (2) Treatment of inoculation materials: sugarcane variety YT94-128 and sugarcane variety GT37 were selected as inoculation materials, and 30 barrels of each variety were planted under greenhouse conditions, with 4 buds per barrel, and the seeds were soaked with 0.3% carbendazim for 20 minutes before planting. Manage normally after emergence, and inoculate when 5 to 6 complete leaves grow.
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