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Preparation method of marine biological protein peptide copper chelate

A technology of marine organisms and protein peptides, applied in animal feed, additional food elements, applications, etc., can solve the problems of low copper loading of protein peptides, low chelation rate of collagen peptide chelated copper products, unfavorable industrialization, etc. Achieve the effect of high chelated copper content, strong implementability and high copper chelation rate

Active Publication Date: 2017-03-29
青岛海大生物集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this study, the chelation rate of collagen peptide chelated copper products is low, and the copper loading capacity of protein peptide is low, which is not conducive to the realization of industrialization

Method used

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  • Preparation method of marine biological protein peptide copper chelate
  • Preparation method of marine biological protein peptide copper chelate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Use a meat grinder to crush 1000kg of marine fish waste into fish paste, pump it into the enzymatic hydrolysis tank, turn on the stirring system, adjust the pH value of the fish paste to 8.0, turn on the heating system to raise the temperature, raise it to 50°C, stop heating and keep it warm, add 1kg Alkaline complex protease, enzymatic hydrolysis for 5h. After the enzymatic hydrolysis reaction, raise the temperature to 90°C, inactivate the enzyme for 30 minutes, centrifuge in a low-speed horizontal centrifuge for 30 minutes, and centrifuge in a high-speed disc centrifuge for 15 minutes, and filter to obtain the enzymatic protein peptide solution. Determine the solid content of the protein peptide solution to be 23%, adjust the pH of the protein peptide solution to 7.0, keep the temperature at 60°C, add 35kg of copper sulfate (pentahydrate), and perform chelation reaction for 3 hours. The reaction solution passes through the nanofiltration system, and then concentrated ...

Embodiment 2

[0026] Use a meat grinder to crush 5000kg of marine fish waste into fish paste, pump it into the enzymatic hydrolysis tank, turn on the stirring system, adjust the pH value of the fish paste to 8.5, turn on the heating system to raise the temperature, raise it to 45°C, stop heating and keep warm, add 5kg Alkaline complex protease, enzymatic hydrolysis for 7h. After the enzymatic hydrolysis reaction, the temperature was raised to 90°C, the enzyme was inactivated for 30 minutes, and the protein peptide solution was obtained by centrifugal filtration. Determine the solid content of the protein peptide solution to be 25%, adjust the pH of the protein peptide solution to 7.0, keep the temperature at 60°C, add 175kg of copper sulfate, and perform the chelation reaction for 3.5 hours. The reaction solution passes through the nanofiltration system, and then concentrated to 50% solids by three-effect concentration, and then spray-dried through the drying tower to obtain chelated copper...

Embodiment 3

[0029] Example 3 Experimental research on the chelation rate of the protein peptide chelated copper product of the present invention and the amount of copper loaded on the protein peptide

[0030] 1. Test material

[0031] Comparative examples; Example 1 group; Example 2 groups.

[0032] experimental method

[0033] 2.1 Determination of chelation rate

[0034] (1) Determination of total copper content

[0035] Principle: After the sample is digested, EDTA can be complexed with copper ions under the condition of pH 5, and the copper content is calculated by indicating the titration end point with 1-(2-pyridylazo)-2-naphthol (PAN) indicator.

[0036] Reagents: nitric acid; hydrochloric acid; disodium edetate standard solution: c(EDTA-2Na)=0.02mol / L; 1-(2-pyridylazo)-2-naphthol (PAN) indicator: 0.2g Dissolve in 100ml 95% ethanol; ammonia water: 10% solution; acetic acid-sodium acetate buffer solution (pH=5): 82g sodium acetate plus 25ml acetic acid, dilute with water to 200ml...

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Abstract

The invention provides a preparation method of marine biological protein peptide copper chelate. The method comprises the following specific steps of: carrying out enzymolysis on sea fish processing leftovers through alkaline compound protease, and carrying out centrifugal filtration to obtain protein peptide solution; adjusting the pH value of the protein peptide solution, and adding soluble copper salt to carry out chelation reaction; and after the chelation reaction is finished, removing free copper ions in the obtained reaction solution through a nano-filtration system, carrying out triple-effect concentration on the filtrate, and carrying out high-temperature spray drying to obtain the marine biological protein peptide copper chelate. The prepared marine biological protein peptide copper chelate is high in copper chelation rate, and can remarkably strength the enzyme activity in the animals and improve the utilization rate of protein, fat and vitamins after being added into feeds, so as to improve the growth performance of the animals.

Description

technical field [0001] The invention relates to a method for preparing marine biological protein peptide chelated copper, which belongs to the technical field of feed additives. Background technique [0002] Copper is one of the trace elements necessary for animals. It participates in the synthesis of hemoglobin and the synthesis and activation of some oxidases in animals. The lack of copper will affect the utilization of iron in animals, resulting in anemia. Therefore, copper as An efficient and inexpensive additive that has been widely used in animal production. However, the first generation of inorganic copper, such as copper sulfate, copper chloride, etc., these inorganic copper additives have a large dosage, low absorption and utilization, and there is antagonism with other nutrients, so animal poisoning, animal product safety and environmental pollution caused by it and other issues have attracted more and more attention. [0003] After the first generation of inorga...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K20/20
Inventor 单俊伟郝晓晨王海华王鹏台文静
Owner 青岛海大生物集团股份有限公司
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