Gracilaria lemaneiformis extract product and preparation method and application thereof

A technology of extract and asparagus, which is applied in the field of asparagus extract and its preparation, can solve the problems that the extract is difficult to separate and purify, limit the biological activity of asparagus, and the active ingredients are easily destroyed, so as to reduce the harm to the human body. The effect of harm, less impurities, and short extraction time

Active Publication Date: 2017-04-05
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the traditional preparation method of asparagus extract has disadvantages such as long time-consuming, high temperature, high energy consumption, large amount of organic solvent, and solution pollutes the environment and product...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Preparation of asparagus extract by ultrasonic extraction process

[0029] A preparation method of asparagus extract, comprising the steps of:

[0030] (1) Preparation of novel ionic liquid 1-butyl-3-methylimidazolium bromide: add N- Methylimidazole and brominated n-butane, with toluene as solvent, under nitrogen protection, add stirring reaction, the stirring speed is 300 rpm, the reaction temperature is 65 °C; after the reaction is completed, cool to room temperature, and the lower layer is yellow viscous Thick 1-butyl-3-methylimidazolium bromide crude product, discard the upper layer solution, first wash twice with ethyl acetate to remove unreacted n-butane bromide, then remove ethyl acetate by rotary evaporation and vacuum dry After 24 hours, the pure product of 1-butyl-3-methylimidazolium bromide, which is a novel ionic liquid, was obtained;

[0031] (2) Ultrasonic extraction of asparagus extract: first take asparagus powder in a beaker, and add the nov...

Embodiment 2

[0032] Example 2 Application of asparagus extract

[0033] An active lead compound for free radical scavenging activity in the fields of marine innovative new drugs, seaweed folk medicines, etc. as a kind of asparagus extract; in particular, it is used for scavenging DPPH free radicals, hydroxyl free radicals ( OH) and superoxide ions ( o 2- ).

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PUM

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Abstract

The invention discloses a gracilaria lemaneiformis extract product, a preparation method and an application thereof. The method comprises the following steps: 1) preparing novel ionic liquid 1-butyl-3-dimethylimidazolium bromides; 2) performing supersonic wave extraction on the gracilaria lemaneiformis extract product; 3) determining the alkaloid of the gracilaria lemaneiformis extract product; and 4) determining the antioxidation activity of the gracilaria lemaneiformis extract product: employing the novel ionic liquid to substitute a traditional organic solvent, the gracilaria lemaneiformis extract product having high purity, less impurity and strong antioxidation activity can be extracted by combining with the supersonic wave technology, The preparation method has the advantages of simple process, short extraction time, low temperature, low energy consumption, and high active ingredient content; recovery rate of total alkaloid in gracilaria lemaneiformis is 95.2%, the clearance of the gracilaria lemaneiformis extract product on DPPH free radical, hydroxy free radical (.OH), super-oxygen ion (O2-)free radical can reach more than 90%; the gracilaria lemaneiformis extract product can be used in the fields of novel sea innovation drugs and alga folk drugs for removing the active lead compounds, and can promote the research and exploitation of sea innovation medicine in our country.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to an asparagus extract and a preparation method and application thereof. Background technique [0002] In recent years, with the rapid development of scientific research in my country and the deepening of human understanding of marine organisms, the development and utilization of marine organisms is one of the hot topics in current scientific research. As we all know, there are many kinds of marine organisms in different shapes, with various biological activities and development and utilization values. The development and utilization of marine organisms can effectively promote the further development of my country's economic development and scientific research. Seaweed is an important part of marine life. Blue-green algae, red algae, brown algae, and blue-green algae are generally called economical algae, but only more than 50 of them have been widely developed and ...

Claims

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Application Information

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IPC IPC(8): A61K36/04A61P39/06
CPCA61K36/04A61K2236/33A61K2236/51
Inventor 贲永光于红王忠黄海兰
Owner GUANGDONG PHARMA UNIV
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