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A kind of mixed cell preparation for treating myocardial infarction and its preparation method and application

A cell preparation, myocardial infarction technology, applied in the field of biomedicine to achieve the effect of repairing the microenvironment of the cell structure, conducive to regeneration, and effective regeneration

Active Publication Date: 2020-04-14
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its efficacy is only reflected in reducing the infarct size, increasing the ejection fraction, improving cardiac function, and improving pathological left ventricular remodeling. However, most of the transplanted stem cells die, and the mature myocardium cannot be differentiated to regenerate the infarcted myocardium. Therefore, the current research and development bottlenecks and frontiers are: research and development can promote myocardial regeneration in ischemic and marginal areas of myocardial infarction, effectively reduce infarct size, improve cardiac function, improve ischemic myocardial fibrosis, and improve the pathological remodeling of infarcted heart, and Cell therapy and cell therapy preparation for effectively reducing heart failure after myocardial infarction

Method used

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  • A kind of mixed cell preparation for treating myocardial infarction and its preparation method and application
  • A kind of mixed cell preparation for treating myocardial infarction and its preparation method and application
  • A kind of mixed cell preparation for treating myocardial infarction and its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0045] 1. Preparation of CTs

[0046] 1. SD female rats (250-300g) (Guangdong Provincial Medical Experimental Animal Center) were killed by pulling the neck after ether anesthesia, soaked in 70 (v / v)% ethanol for 5 minutes, and quickly transferred to a biological safety cabinet.

[0047] 2. Under sterile conditions, quickly cut open the chest cavity and take out the heart, put the heart into sterile PBS solution (0.01M, pH7.4) and wash it 3 times to remove the residual blood in the heart, and cut the heart tissue in a sterile plate (1-2mm 3 ), then transfer the heart tissue block to a 50ml sterile centrifuge tube, add 37°C preheated digestive enzyme solution [0.05 (w / v)% collagenase + 0.1 (w / v)% trypsin, The same below] 2.5ml, after sealing the tube cap, digest it in a water-bath shaker (300rpm) at 37°C for 10min, then let it stand in a biological safety cabinet for 2min, and discard the supernatant suspension.

[0048] 3. In the biosafety cabinet, add 2.5ml of digestive enz...

Embodiment 2

[0077] (1) Preparation of CTs+CSCs mixed cell preparation

[0078] Mix CTs and CSCs with sterile DMED medium containing 20% ​​(v / v) fetal bovine serum, and press 1.25×10 5 :1.25×10 5 The ratio of the two cells was evenly mixed to a volume of 2mL and a cell concentration of 1.25×10 5 / mL of cell mixture.

[0079] Place the mixed cell solution at 37°C, 5% CO 2 , 95% air in a sterile incubator for 48 hours, then use 0.25 (w / v)% trypsin to digest and recover the mixed cultured cells, and use DMEM containing 20% ​​(v / v) fetal bovine serum to reduce the cell concentration Modulation is 1×10 7 / mL for transplantation.

[0080] (2) Transplantation of CTs+CSCs mixed cell preparation for myocardial infarction

[0081] Female (250 g) S.D. rats were used to model myocardial infarction after ligation of the anterior descending coronary artery (LAD). Intramyocardial injection of the CTs+CSCs mixed cell preparation prepared in step (1) into the ischemic marginal zone, wherein 10 μl wa...

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Abstract

The invention discloses a mixed cell preparation used for treating myocardial infarction as well as a preparation method thereof and an application thereof. The mixed cell preparation consists of active ingredients and a solvent, wherein the active ingredients are mainly CTs and CSCs. The mixed cell preparation is transplanted for an ischemic region and an ischemic penumbra region of a heart with acute myocardial infarction, so that survival, differentiation and multiplication, on the ischemic penumbra region, of transplanted CSCs or myocardial endogenous CSCs and other derived stem cells can be effectively promoted, and a severely damaged cell network which consists of CTs of a regenerative ischemic myocardium ischemic region is effectively repaired, and therefore, regeneration of infarcted myocardium is facilitated. The mixed cell preparation has curative effect, which is superior to that of independent use of CSCs, BMSCs and CTs, in reducing an infarct size and improving the function of a myocardial infarction heart, and the like after implementing transplantation therapy on acute myocardial infarction. Therefore, the mixed cell preparation can be prepared into a drug for treating myocardial infarction or applied to mechanical apparatuses and instruments.

Description

technical field [0001] The invention relates to a mixed cell preparation for treating myocardial infarction, a preparation method and application thereof, and belongs to the field of biomedicine. Background technique [0002] At present, regardless of the basic and clinical aspects, although the treatment of myocardial infarction has made great progress in recent decades, there is still no drug or therapy that can cure myocardial infarction. Cell therapy is considered to be one of the most promising treatments for myocardial infarction. Both basic and clinical studies have proved that different types of stem cells (including: bone marrow mesenchymal stem cells, cardiac endogenous stem cells, embryonic stem cells and iPS, etc.) are feasible and safe for the treatment of infarcted myocardium. However, its efficacy is only reflected in reducing the infarct size, increasing the ejection fraction, improving cardiac function, and improving pathological left ventricular remodeling...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K35/34A61K31/7088A61P9/10
Inventor 蔡冬青廖肇福赵宝寅沈晓涛郑馨陈夷林李艳梅许甘霖齐绪峰徐莎莎
Owner JINAN UNIVERSITY
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