Nano flower-like palladium-gold coated betulinic acid liposome and preparation method thereof

A technology of nano-liposome and betulinic acid, which is applied in the field of medicine, can solve the problem of killing tumor cells, etc., and achieve the effects of simple operation, good photothermal conversion performance and high repeatability

Active Publication Date: 2017-05-10
YANSHAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, photothermal therapy alone is not sufficient to kill tumor cells

Method used

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  • Nano flower-like palladium-gold coated betulinic acid liposome and preparation method thereof
  • Nano flower-like palladium-gold coated betulinic acid liposome and preparation method thereof
  • Nano flower-like palladium-gold coated betulinic acid liposome and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] With 4mg betulinic acid (purchased from Nanjing Zelang Biotechnology Co., Ltd.), 5.2mg cholesterol (purchased from Tianjin Damao Chemical Instrument Supply Station) and 48mg soybean lecithin (purchased from Shanghai Everett Pharmaceutical Technology Co., Ltd.), Dissolve in 4mL of absolute ethanol and perform ultrasonication, ultrasonic power 40W, ultrasonic 10min, to obtain a lipid solution; take 6mg of glutathione dissolved in 20mL of deionized water to obtain a glutathione solution; 38 ° C magnetic stirring, 10mL Lipid solution was added dropwise to 15mL glutathione solution to obtain liposome suspension; then the obtained liposome suspension was stirred at 28°C and 100rpm for 3 hours to remove liposome suspension The dehydrated alcohol in, obtains the betulinic acid nano liposome of glutathione modification;

[0028] Add 10 μL of ascorbic acid solution with a molar concentration of 50 mM dropwise into 1000 μL of a palladium chloride solution with a molar concentratio...

Embodiment 2

[0032] With 4mg betulinic acid (purchased from Nanjing Zelang Biotechnology Co., Ltd.), 6.8mg cholesterol (purchased from Tianjin Damao Chemical Instrument Supply Station) and 64mg soybean lecithin (purchased from Shanghai Aiweite Medical Technology Co., Ltd.), Dissolve in 4 mL of absolute ethanol and perform ultrasonication at 60 W for 15 min to obtain a lipid solution. Dissolve 12mg of glutathione in 20mL of deionized water to obtain glutathione solution; 42°C magnetic stirring, add 10mL of lipid solution dropwise to 25mL of glutathione solution to obtain liposome suspension; Subsequently, the obtained liposome suspension was magnetically stirred at 31° C. and 115 rpm for 5 hours to remove absolute ethanol in the liposome suspension to obtain glutathione-modified betulinic acid nanoliposomes;

[0033] 25 μL of ascorbic acid solution with a molar concentration of 75 mM was added dropwise to 1000 μL of a palladium chloride solution with a molar concentration of 1 mM, and mixed...

Embodiment 3

[0037] With 4mg betulinic acid (purchased from Nanjing Zelang Biotechnology Co., Ltd.), 8.4mg cholesterol (purchased from Tianjin Damao Chemical Instrument Supply Station) and 80mg soybean lecithin (purchased from Shanghai Aiweite Medical Technology Co., Ltd.), Dissolve in 4mL of absolute ethanol and ultrasonicate 80W for 20min to obtain a lipid solution; dissolve 20mg of glutathione in 20mL of deionized water to obtain a glutathione solution; Add dropwise in 35mL glutathione solution, obtain liposome suspension. Subsequently, the obtained liposome suspension was magnetically stirred at 35° C. and 130 rpm for 7 hours to remove absolute ethanol in the liposome suspension to obtain glutathione-modified betulinic acid nanoliposomes;

[0038] Add 40 μL of ascorbic acid solution with a molar concentration of 100 mM dropwise into 1000 μL of a palladium chloride solution with a molar concentration of 1.5 mM, mix quickly and evenly until the color turns light green, and obtain a palla...

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Abstract

A nano flower-like palladium-gold coated betulinic acid liposome is a nano liposome formed by forming a nano flower-like palladium-gold alloy shell on the surface of a betulinic acid nano liposome, with the particle size of about 80-100 nm and the near infrared absorption wavelength of 500-900 nm. A preparation method mainly comprises steps as follows: palladium nanoparticles are obtained by reducing a palladium chloride solution with ascorbic acid, the palladium nanoparticles and the betulinic acid nano liposome modified with glutathione are subjected to shaded incubation under the mild condition, then a gold chloride solution and a hydroxylamine hydrochloride solution are added, and shaded incubation is performed for 4-9 h. The preparation method is simple to operate, easy to control and high in repeatability; the prepared nano flower-like palladium-gold coated betulinic acid liposome has good photo-thermal conversion performance, makes medicine release controllable, is more effective and low-toxic and can combine photo-thermal therapy and chemotherapy to treat cancer collaboratively.

Description

technical field [0001] The invention belongs to the technical field of medicine, in particular to a nano liposome and a preparation method thereof. Background technique [0002] Cancer is one of the major malignant diseases that currently threaten human health. The traditional chemotherapy drugs currently used have serious side effects, and the emergence of nanotechnology provides new ideas for the treatment of cancer. Among them, liposome is a non-toxic, biocompatible and promising nano-drug delivery system. However, in order to achieve a better therapeutic effect, conventional liposomes still have some defects, such as instability, poor targeting, and uncontrollable drug release. [0003] In recent years, various photothermal materials have been gradually applied in the field of biomedicine. Among them, nanomaterials that respond to near-infrared light are the most widely studied, such as noble metal nanomaterials (gold nanomaterials), graphene, carbon nanotubes, and rar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K31/56A61K9/127A61K9/50A61P35/00
Inventor 高大威刘艳平张旭武李磊何雨初
Owner YANSHAN UNIV
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