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Bi-component quick haemostatic gel and application thereof

A hemostatic gel, two-component technology, applied in the field of two-component rapid hemostatic gel, can solve the problems of poor mechanical strength and tissue binding force of the gel, and achieves reduction of gel formation time, simple usage, and biological safety. Good results

Active Publication Date: 2017-05-10
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a two-component rapid hemostatic gel and its preparation method and application, so as to improve the problems of poor mechanical strength and tissue binding force of the existing gel

Method used

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  • Bi-component quick haemostatic gel and application thereof
  • Bi-component quick haemostatic gel and application thereof
  • Bi-component quick haemostatic gel and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1) Dissolve 1g of chitosan in 100ml of water with a pH of 6 to prepare a solution with a mass concentration of 1%. After it is completely dissolved, add 0.5g of sodium periodate, react in the dark at 20°C for 1 hour, and then add 0.9ml of ethylene glycol Stop the reaction. Pour the reaction solution into a dialysis bag (molecular weight permeation 8000-14000Da) with deionized water as the dialysate, dialyze at 15°C for 2 days, then take the retentate and store it at -20°C for 0.5 hours, then store it at -80°C Stand for 5 hours, then freeze-dry in a freeze dryer at -50°C and 1Pa for 24 hours to obtain aldehydated chitosan, the oxidation rate of aldehyde groups (that is, the theoretical mass content of aldehyde groups accounts for the mass of aldehylated natural polysaccharides) 40%.

[0027] 2) Dissolve 1 mmol of hyaluronic acid in 100 ml of deionized water, and then add 1 mmol of hydrazine. After complete mixing, a mixture of 1 mmol of 1-(3-dimethylaminopropyl)-3-ethy...

Embodiment 2

[0030] 1) Dissolve 10g of dextran in 100ml of deionized water to prepare a solution with a mass concentration of 10%. After it is completely dissolved, add 10g of sodium perchlorate and react in the dark at 25°C for 3 hours, then add 9ml of ethylene glycol to terminate the reaction. Pour the reaction solution into a dialysis bag (molecular weight permeation 8000-14000Da) with deionized water as the dialysate, dialyze at 25°C for 7 days, then store it at -20°C for 3 hours, then place it at -80°C for 12 hours , and then lyophilized in a freeze dryer under the conditions of -50°C and 99Pa for 96 hours to obtain aldylated dextran, and the oxidation rate of the aldehyde group was 50%.

[0031] 2) Dissolve 1 mmol of gelatin (purchased from sigma) in 100 ml of deionized water, and then add 30 mmol of adipic acid dihydrazide. After complete mixing, a mixture of 5 mmol 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and 5 mmol 1-hydroxybenzotriazole hydrate (HOBt) was...

Embodiment 3

[0034] 1) Dissolve 2g of hyaluronic acid in 100ml of deionized water to prepare a solution with a mass concentration of 2%. After it is completely dissolved, add 2g of potassium periodate, react in the dark at 30°C for 12 hours, and then stop with 1.8ml of ethylene glycol reaction. Pour the reaction solution into a dialysis bag (molecular weight permeation 8000-14000Da) with deionized water as the dialysate, dialyze at 15°C for 4 days, then store it at -20°C for 1.5 hours, then place it at -80°C for 10 hours , and then lyophilized in a freeze dryer under the conditions of -50° C. and 50 Pa for 48 hours to obtain alhyallated hyaluronic acid, and the oxidation rate of the aldehyde group was 42%.

[0035] 2) Dissolve 1 mmol of sodium alginate in 100 ml of deionized water, and then add 15 mmol of adipic acid dihydrazide. After complete mixing, a mixture of 3 mmol 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and 3 mmol N-hydroxysuccinimide (NHS) was added to a...

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Abstract

The invention discloses a bi-component quick haemostatic gel and an application thereof. The gel is composed of natural aldehyde polysaccharide solution in mass concentration of 1-20% and amino modified natural biomacromolecule solution in mass concentration of 1-20% at volume ratio of 1:(0.1-1). According to the invention, the stickiness of the gel can be increased and the gelling time can be reduced in the manner of increasing the aldehyde content in the natural polysaccharide and the concentration of the natural aldehyde polysaccharide solution; the gelling time can be reduced in the manner of increasing the grafting volume of amino group in the amino modified natural biomacromolecule solution and the concentration of the amino modified natural biomacromolecule solution. According to the invention, the modified biomacromolecule and the natural biomacromolecule are adopted, so that the biological safety is high; the use method is simple; the bi-component quick haemostatic gel can be applied to the medical haemostatic field and can form the gel for stopping bleeding within 10-600 seconds.

Description

[0001] (1) Technical field [0002] The invention relates to a two-component rapid hemostatic gel and its application. [0003] (2) Background technology [0004] Rapid hemostasis is an important requirement in clinical medicine, which is directly related to the life safety of patients. The use of rapid hemostasis materials can effectively reduce the death rate of bleeding personnel. According to the mechanism of hemostasis, the following four methods of hemostasis are commonly used at present: First, by promoting vasoconstriction, reducing capillary permeability, reducing blood flow, and promoting blood coagulation. The second is to increase the number of platelets, enhance their aggregation and adhesion, and promote the release of coagulation factors from platelets to achieve the purpose of hemostasis. The third is to promote the coagulation system to activate prothrombin to form thrombin, and thrombin catalyzes fibrinogen to form fibrin, so as to achieve the purpose of hemo...

Claims

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Application Information

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IPC IPC(8): A61L24/04A61L24/00C08B37/08C08B37/02C08B37/04C08H1/00C08B15/02
CPCA61L24/0031A61L24/043A61L2400/04C08B15/02C08B37/0006C08B37/003C08B37/0069C08B37/0072C08B37/0084C08H1/00
Inventor 洪逸周飞飞章淑芳欧阳宏伟
Owner ZHEJIANG UNIV
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