A microbial remediation method suitable for cyfluthrin pollution in marine environment
A technology of cyfluthrin and microbial remediation, which is applied in the field of marine environmental governance, can solve the problems of reducing the adsorption effect of trace pollutants, and achieve the effects of improving microbial degradation efficiency, eliminating pollution, and good hydrophilicity
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[0019] A microbial remediation method suitable for marine environment cyfluthrin pollution, comprising the following steps:
[0020] 1. Isolation, screening and domestication of highly efficient cyfluthrin-degrading bacteria
[0021] Isolation and domestication of a cyfluthrin-degrading bacterium in a seawater environment: Add 100mL of nutrient culture solution into a 300mL Erlenmeyer flask, and then add pesticide stock solution to make the substrate concentration 100mg / L. Then add 2 g of sediment samples or microorganisms obtained through seawater, and culture at 28° C. with constant temperature shaking at 180 rpm. Transfer 10% bacterial solution by volume to 90mL fresh nutrient medium every 7 days. Stable flora obtained through continuous enrichment culture, 0.1mL of the culture solution was taken to separate and purify by streaking on the plate, and the Photobacterium acuminata was isolated, which was inoculated on the slant medium of the test tube and stored at 4°C at low...
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