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A microbial remediation method suitable for cyfluthrin pollution in marine environment

A technology of cyfluthrin and microbial remediation, which is applied in the field of marine environmental governance, can solve the problems of reducing the adsorption effect of trace pollutants, and achieve the effects of improving microbial degradation efficiency, eliminating pollution, and good hydrophilicity

Active Publication Date: 2019-10-08
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Immobilized microbial treatment technology can exert the carrier's adsorption and enrichment effect on trace pollutants, and increase the substrate concentration required for microbial degradation. However, commonly used immobilized carriers such as activated carbon and carbon nanotubes are non-specific, especially It is the existence of complex matrix in the environment that reduces the adsorption effect of trace pollutants

Method used

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specific Embodiment

[0019] A microbial remediation method suitable for marine environment cyfluthrin pollution, comprising the following steps:

[0020] 1. Isolation, screening and domestication of highly efficient cyfluthrin-degrading bacteria

[0021] Isolation and domestication of a cyfluthrin-degrading bacterium in a seawater environment: Add 100mL of nutrient culture solution into a 300mL Erlenmeyer flask, and then add pesticide stock solution to make the substrate concentration 100mg / L. Then add 2 g of sediment samples or microorganisms obtained through seawater, and culture at 28° C. with constant temperature shaking at 180 rpm. Transfer 10% bacterial solution by volume to 90mL fresh nutrient medium every 7 days. Stable flora obtained through continuous enrichment culture, 0.1mL of the culture solution was taken to separate and purify by streaking on the plate, and the Photobacterium acuminata was isolated, which was inoculated on the slant medium of the test tube and stored at 4°C at low...

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Abstract

The invention discloses a microbial remediation method suitable for marine environment polluted by cyfluthrin, which is characterized in that it comprises the following steps: (1) Screening and acclimating to obtain a highly efficient cyfluthrin-degrading bacterium, the bacterium is Nbuzhang1 strain, and the classification name is Gangua Photobacteria; (2) Using the reverse microemulsion polymerization method to obtain a molecularly imprinted-quantum dot nanostructure polymer with specific recognition properties for bifluthrin; (3) Adding highly efficient cyfluthrin-degrading bacteria to the molecularly imprinted cyfluthrin ‑Quantum dot nanostructure polymers, adsorb and fix microorganisms to form immobilized microorganisms; (4) add immobilized microorganisms to an appropriate amount of polluted seawater, adjust the pH value of polluted seawater to 7‑8, and stand still for 2 hours. The advantage is that it has better Hydrophilicity, biocompatibility and high adsorption capacity allow for the selective adsorption of cyfluthrin from the culture environment.

Description

technical field [0001] The invention relates to the technical field of marine environment treatment, in particular to a microbial remediation method suitable for cyfluthrin pollution in the marine environment. Background technique [0002] Pyrethroid pesticides were once considered to have the advantages of high efficiency, low toxicity, and safe use, and could be used as substitutes for organochlorine and organophosphorus pesticides. However, recent studies have shown that such pesticides are accumulative, and long-term exposure can easily lead to chronic diseases. Carcinogenic, teratogenic, mutagenic risks. At the same time, due to the strong lipophilicity of this type of pesticide, it is highly toxic to aquatic organisms such as fish and shellfish. In particular, toxic substances with endocrine disrupting properties such as bifluthrin have an activity similar to biological hormones, and a very small amount of residue can cause toxicity to the endocrine system of organism...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/38C02F101/36C02F101/34C02F103/08
CPCC02F3/34C02F2101/34C02F2101/36C02F2101/38C02F2103/08C12N1/20C12N1/205C12R2001/01
Inventor 史西志张蓉蓉孙爱丽郭晓青李德祥陈炯
Owner NINGBO UNIV