System device for strengthening high-viscosity microbial polysaccharide fermentation and application

A microbial polysaccharide and system device technology, which is applied in the field of system devices to strengthen the fermentation of high-viscosity microbial polysaccharides, can solve the problems of limited mass transfer and mixing, long fermentation cycle of high-viscosity polysaccharides, low conversion rate and yield, etc., to achieve shortened fermentation cycle, increase yield and quality, and reduce apparent viscosity

Inactive Publication Date: 2017-05-10
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention proposes a system device for strengthening the fermentation of high-viscosity microbial polysaccharides, and uses it to ferment and produce high-viscosity exopolysaccharides, which overcomes the limitations of mass transfer and mixing in existing high-viscosity polysaccharide fermentation technologies, and solves the problem The problems of long fermentation period, low conversion rate and yield of high-viscosity polysaccharides have important practical significance and good application prospects

Method used

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  • System device for strengthening high-viscosity microbial polysaccharide fermentation and application
  • System device for strengthening high-viscosity microbial polysaccharide fermentation and application
  • System device for strengthening high-viscosity microbial polysaccharide fermentation and application

Examples

Experimental program
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Effect test

Embodiment 1

[0045] see figure 1 As shown, a system device for strengthening the fermentation of highly viscous microbial polysaccharides includes a tank body 1, a tank cover 2, a feeding port 3, an inoculation port 4, an exhaust pipeline 5, a second motor 6, and a first motor 7 , air / steam input pipeline 8, temperature sensor 9, pH electrode 10, dissolved oxygen electrode 11, annular liquid receiving tank 12, end cover 13, cooling interlayer 14, turntable 15, air distributor 16, material intake port 17, High temperature resistant submerged pump 18, lifter 19, liquid distributor 20, first pin 21, second pin 22.

[0046] The inner center of the tank body 1 is longitudinally provided with a rotating shaft, the upper end of the rotating shaft passes through the tank cover 2 and is connected to the first motor 7, and the lower end extends into the tank body 1 to connect with the supergravity rotating bed device; the supergravity rotating bed device is horizontally installed in the tank The in...

Embodiment 2

[0054] Taking xanthan gum as a representative and adopting the system device of embodiment 1 to strengthen the viscosity range is 5.0×10 4 ~1.6×10 5 The exopolysaccharide biological fermentation process of mPa·s, the specific steps are as follows:

[0055] Incline medium: sucrose 20g / L, peptone 5g / L, yeast extract powder 5g / L, beef powder 3g / L, agar 20g / L.

[0056] Seed medium: sucrose 20g / L, peptone 5g / L, yeast extract powder 5g / L, beef powder 3g / L.

[0057] Fermentation medium: sucrose 20g / L, glucose 30g / L, peptone 3g / L, potassium chloride 25g / L, magnesium sulfate 8g / L, calcium carbonate 10g / L, citric acid 0.25g / L, potassium dihydrogen phosphate 0.06 g / L.

[0058] 1) Put Xanthomonas BT-112, a slanted bacteria strain stored in a refrigerator at 4°C, into a shaker flask (50mL / 250mL) with seed medium, place it in a constant temperature culture shaker, and set it at 30°C, 190rpm Cultivate under the same conditions for 26 hours as the first-level seeds, insert the above-menti...

Embodiment 3

[0066] Taking hyaluronic acid as a representative, the system device of Example 1 is used to strengthen the viscosity range of 1.0×10 3 -5.0×10 4 The exopolysaccharide biological fermentation process of mPa·s, the specific steps are as follows:

[0067] Incline medium: sucrose 30g / L, peptone 10g / L, yeast extract powder 5g / L, magnesium sulfate 1g / L, dipotassium hydrogen phosphate 1.5g / L, sodium glutamate 2g / L, agar 20g / L.

[0068] Seed medium: sucrose 30g / L, peptone 10g / L, yeast extract powder 5g / L, magnesium sulfate 1g / L, dipotassium hydrogen phosphate 1.5g / L, sodium glutamate 2g / L, sodium bicarbonate 1g / L .

[0069] Fermentation medium: sucrose 40g / L, peptone 10g / L, magnesium sulfate 0.5g / L, dipotassium hydrogen phosphate 1.5g / L, sodium glutamate 2.5g / L, sodium bicarbonate 0.5g / L.

[0070] 1) Insert Klebsiella spp. H-112, which was stored in a refrigerator at 4°C, into the seed medium and culture it at 34°C and 160rpm for 13 hours as the primary seed, and insert the above ...

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Abstract

The invention discloses a system device for strengthening high-viscosity microbial polysaccharide fermentation. The system device comprises a tank body, a tank cover, a cooling interlayer, an air distributor, a first motor, a second motor, a supergravity revolving bed device and a high-temperature-resisting submerged pump. A rotary shaft is longitudinally arranged inside the tank body. The upper end of the rotary shaft penetrates through the tank cover to be in driven connection with the first motor, and the lower end of the rotary shaft extends into the tank body to be connected with the supergravity revolving bed device. The supergravity revolving bed device is transversely arranged at the center of the inside of the tank body. The air distributor is arranged at the lower end of the supergravity revolving bed device and is an annular pipeline. The high-temperature-resisting submerged pump is arranged on one side of the lower end of the inside of the tank body. One end of the high-temperature-resisting submerged pump is connected with the supergravity revolving bed device through a pipe, and the other end of the high-temperature-resisting submerged pump is connected with a rotary shaft of the second motor. The cooling interlayer is arranged outside the tank body. A fermentation period is greatly shortened by strengthening dissolved oxygen mass transfer, gas, liquid and solid phase mixing, rotating speed optimization, ventilatory capacity and other conditions, the yield and mass of high-viscosity polysaccharide are increased, and the cost is remarkably reduced.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation. More specifically, it relates to a system device and application for strengthening the fermentation of high-viscosity microbial polysaccharides. Background technique [0002] Microbial exopolysaccharide refers to the polysaccharide or polysaccharide mixture secreted by microorganisms outside the cell wall during metabolic activities. It is a new fermentation product developed in the past 20 years. It has special rheological characteristics and excellent biological activity, and is safe and non-toxic. Many highly viscous microbial polysaccharides are widely used in food, pharmaceutical, petroleum, chemical and other fields due to their thickening, stability, cohesion and suspension properties. With the increasing emphasis on environmental protection and human health in modern society, the demand for such microbial polysaccharide products is growing rapidly. It is estimated that th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/04C12M1/34C12M1/02C12P19/04
CPCC12M27/10C12M29/06C12M41/22C12M41/26C12M41/32C12P19/04
Inventor 张丽丽李旭初广文邹海魁孙宏帅
Owner BEIJING UNIV OF CHEM TECH
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