Method for removing heavy metal in marine animal homogenate

A technology for removing heavy metals from marine animals, applied in microorganism-based methods, methods using microorganisms, biochemical equipment and methods, etc., can solve the problems of difficulty in separating the remover and the removal material, and achieve easy filtration and removal. and reuse, high removal rate, and the effect of ensuring safety

Active Publication Date: 2017-05-10
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the microbial method also has the difficulty of separating the removal agent from the removal liquid

Method used

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  • Method for removing heavy metal in marine animal homogenate
  • Method for removing heavy metal in marine animal homogenate
  • Method for removing heavy metal in marine animal homogenate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The method for removing heavy metals in oyster homogenate, the specific steps are as follows:

[0040] 1) Cultivation of Pichia Kudori and Preparation of Yeast Sludge

[0041] Slant activation: inoculate the strains stored at 4°C into YPD slant medium, and culture in a constant temperature incubator at 28°C for 24 hours;

[0042] Activation of liquid medium: Scrape the activated bacteria from the inclined surface with the inoculation loop and inoculate a small amount into the YPD liquid medium, and cultivate it in a constant temperature shaking incubator at 28°C and 180r / min for 24h to form a seed liquid;

[0043] Sodium chloride stress culture: Take 1ml of the activated yeast seed solution, inoculate it into YPD liquid medium containing 8% sodium chloride, and cultivate it at 28°C and 180r / min for 20h.

[0044] Collect the sludge: After 20 hours of sodium chloride stress culture, use a hemocytometer to quantify. Take a certain volume of yeast suspension and centrifug...

Embodiment 2

[0055] The method for removing heavy metals in sea cucumber intestinal homogenate, concrete steps are as follows (as figure 1 shown):

[0056] 1) Cultivation of Pichia Kudori and Preparation of Yeast Sludge

[0057] Slant activation: inoculate the strains stored at 4°C into YPD slant medium, and culture in a constant temperature incubator at 28°C for 24 hours;

[0058] Activation of liquid medium: Scrape the activated bacteria from the inclined surface with the inoculation loop and inoculate a small amount into the YPD liquid medium, and cultivate it in a constant temperature shaking incubator at 28°C and 180r / min for 24h to form a seed liquid;

[0059] Sodium chloride stress culture: Take 1ml of the activated yeast seed solution, inoculate it into YPD liquid medium containing 8% sodium chloride, and cultivate it at 28°C and 180r / min for 20h.

[0060] Collect the sludge: After 20 hours of sodium chloride stress culture, use a hemocytometer to quantify. Take a certain volume...

Embodiment 3

[0075] The method for removing heavy metals in sea cucumber egg homogenate, the specific steps are as follows:

[0076] 1) Cultivation of Pichia Kudori and Preparation of Yeast Sludge

[0077] Slant activation: inoculate the strains stored at 4°C into YPD slant medium, and culture in a constant temperature incubator at 28°C for 24 hours;

[0078] Activation of liquid medium: Scrape the activated bacteria from the inclined surface with the inoculation loop and inoculate a small amount into the YPD liquid medium, and cultivate it in a constant temperature shaking incubator at 28°C and 180r / min for 24h to form a seed liquid;

[0079] Sodium chloride stress culture: Take 1ml of the activated yeast seed solution, inoculate it into YPD liquid medium containing 8% sodium chloride, and cultivate it at 28°C and 180r / min for 20h.

[0080] Collect the sludge: After 20 hours of sodium chloride stress culture, use a hemocytometer to quantify. Take a certain volume of yeast suspension and...

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Abstract

The invention provides a method for removing heavy metal in marine animal homogenate. The method comprises the steps of firstly performing expansion culturing on yeast, preparing immobilized yeast gel balls, and preparing marine animal homogenate; performing acidolysis on the marine animal homogenate and separating the processed homogenate to acquire supernate and bottom sediment; and at last removing the heavy metal in the bottom sediment by using the yeast gel balls, and adsorbing the heavy metal in the supernate by using the free yeast. According to the method provided by the invention, aiming at the homogenate of marine organism, the steps of centrifugally separating the homogenate into the supernate part and the sediment part for processing, using yeast mud to remove the heavy metal in the supernate and removing the heavy metal in the sediment by using the yeast gel balls are ingeniously provided; and compared with the process of removing the heavy metal from the overall homogenate, the method provided can be significantly improved in heavy metal removal rate. Microorganism is immobilized, adsorptive property of the microorganism to the heavy metal can be achieved, and separation between the microorganism and foods can also be better achieved, so that the method is convenient for industrial utilization.

Description

technical field [0001] The invention relates to a method for removing heavy metals from marine animal homogenate, and belongs to the field of food biotechnology. Background technique [0002] With the development of industry and the increase of human activities, heavy metal pollution in the atmosphere, water, soil and other environments is becoming more and more serious, and the problem of heavy metal pollution in water environment is very prominent. Heavy metal pollution in the water environment has caused varying degrees of damage to the aquatic ecosystem. Many aquatic organisms, including fish, shrimp, and shellfish, have a high enrichment effect on heavy metals in their growth environment, resulting in heavy metal pollution in aquatic products. Incidents occur from time to time, and heavy metal pollution has become one of the important factors affecting the safety of aquatic products. Heavy metals cannot be degraded in the environment, and can accumulate in organisms, c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/18C12N1/16C12N1/36C12N11/10C12N11/04A23L5/20A23L17/00A23L17/50C12R1/84C12R1/865C12R1/645
CPCA23V2002/00C12N1/16C12N1/18C12N1/36C12N11/04C12N11/10A23V2250/76A23V2250/762
Inventor 徐莹张丹丹汪东风赵文玉包尊
Owner OCEAN UNIV OF CHINA
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