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Method for efficient extraction of active protein

An active protein and high-efficiency technology, applied in the direction of fermentation, can solve the problems of complicated operation steps, low protein purity, and protein extraction rate to be improved, and achieve the effect of high purity, high protein activity, and small precipitation range.

Inactive Publication Date: 2017-05-10
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the purity of protein extracted by this method is not high, and the protein extraction rate needs to be improved, and the operation steps are cumbersome

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A method for efficiently extracting active protein

[0015] 1) Pretreatment: Sun-dried Moringa oleifera seeds, peeled to obtain white spherical inner seeds, soaked in deionized water, dried, ultrafinely pulverized, and sieved. Deionized water can effectively remove water-soluble impurities and prepare for the extraction of active protein, so that the extracted active protein has high purity; 2) Degreasing: add degreasing agent and active agent to the inner seed powder, stir magnetically, and the stirring rate is 40~ 60r / min, for 20~40min, let it stand for 1~2 days, filter to obtain blocky powder, then spread the powder on oil-absorbing paper, let it dry naturally in a ventilated place, and pass through a 60~80 mesh sieve. Compatible with the same sex, this method can not only remove oil well, but also maintain protein activity;

[0016] 3) Extract active protein: Add Tris-HCl buffer solution with a volume ratio of 1:45 to 1:55 and a concentration of 1.4 to 1.6 mol / L to...

Embodiment 2

[0022] 1) Pretreatment: Sun-dried Moringa oleifera seeds, peeled to obtain white spherical inner seeds, soaked in deionized water, dried, ultrafinely pulverized, and sieved. Deionized water can effectively remove water-soluble impurities and prepare for the extraction of active protein, so that the extracted active protein has a high purity; 2) Degreasing: add degreasing agent and active agent to the inner seed powder, stir magnetically, and the stirring rate is 60r / min, last for 20 minutes, let stand for 1~2 days, filter to get lumpy powder, then spread the powder on oil-absorbing paper, air-dry naturally in a ventilated place, and pass through an 80-mesh sieve. Compatible with the same sex, this method can not only remove oil well, but also maintain protein activity;

[0023] 3) Extract active protein: add Tris-HCl buffer solution with a volume ratio of 1:50 and a concentration of 1.5mol / L to the sieved inner seed powder, stir magnetically at a stirring rate of 40r / min, and...

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Abstract

The invention provides a method for efficient extraction of active protein. The method comprises steps of 1) conducting pre-processing; 2) conducting degreasing; 3) extracting the active protein; and 4) preserving the active protein in a freeze-dried mode. The method provided by the invention has the beneficial effect that an enzymatic hydrolysis agent, a cosolvent and a separating agent are added to the step of extracting the active protein. The enzymatic hydrolysis agent consists of the following components: 8-15 parts of papain, 1-2 parts of cysteine, 0.5-0.8 part of sodium sulfite and 2-4 parts of glucosamine, and the enzymatic hydrolysis agent is capable of rapidly hydrolyzing moringa seed protein; the cosolvent consists of the following components: 5-10 parts of sodium salicylate, 1-3 parts of magnesium chloride and 2-5 parts of ammonium phosphate, and the cosolvent can form a water-soluble intermolecular complex with the active protein in moringa seeds, so as to improve the solubility of the active protein and increase the extracting rate of the active protein; and the separating agent consists of the following components: 3-5 parts of ammonium sulfate, 1-3 parts of sodium chloride, 0.5-1 part of potassium nitrate and 2-5 parts of vitamin C, and the separating agent is low in protein separating range, high in purity of the separated active protein, high in separating rate, which is 98% or above, and high in protein activity.

Description

technical field [0001] The invention relates to the technical field of active protein extraction, in particular to a method for efficiently extracting active protein. Background technique [0002] Moringa seed is the seed of Moringa tree native to India with extremely harsh natural conditions. Moringa tree is called the "miracle tree" and also known as the drumstick tree. It is rich in nutrients and high in vitamins A, B, C, and E , high protein, high fiber. According to research, the mass fraction of each component in Moringa seed is 33.5% of Moringa oil, 37.9% of Moringa protein, 6.1% of starch, 17.1% of total soluble sugar (including 12.3% of Moringa polysaccharide), 7.5% of crude fiber and rich Macro elements such as: calcium, potassium, magnesium, sodium, phosphorus and trace elements such as: zinc, copper, iron, etc. The active protein in Moringa seeds has very strong antioxidant properties, can remove free radicals, and play a role in removing freckles, whitening an...

Claims

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Application Information

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IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 孙坤来杨帆陈子阳陈常健宋丽婉宣丹维董严王斌陈荫赵玉勤
Owner ZHEJIANG OCEAN UNIV
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