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Preparation method of collagen-hydroxyapatite artificial bone

A hydroxyapatite and artificial bone technology, applied in prosthesis, tissue regeneration, medical science and other directions, can solve the problem that the material can not fully meet the clinical repair and other problems, achieve the promotion of adhesion and proliferation, good mechanical properties, and improve the degree of deacetylation Effect

Inactive Publication Date: 2017-05-24
福州市大福瑞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to various defects, these materials cannot fully meet the requirements of clinical repair.

Method used

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  • Preparation method of collagen-hydroxyapatite artificial bone
  • Preparation method of collagen-hydroxyapatite artificial bone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A preparation method of collagen-hydroxyapatite artificial bone, specifically comprising the following steps:

[0026] (1) Polylactic acid was prepared into a 1wt% solution with tetrahydrofuran;

[0027] (2) Prepare chitosan, and prepare the prepared chitosan into a 0.02wt% solution with dilute acetic acid;

[0028] (3) Prepare a collagen solution with a concentration of 8g / L and a keratin solution with a concentration of 1g / L with distilled water;

[0029] (4) Add hydroxyapatite to the mixed solution of 8g / L collagen and 1g / L keratin, adjust the pH to 7.0 with sodium hydroxide solution, and add 1.5wt% genipin aqueous solution at 4 Cross-linking at ℃ for 2 hours to obtain mixed solution A, freeze-drying to obtain collagen / keratin / hydroxyapatite composite powder with a mass ratio of collagen, keratin and hydroxyapatite of 0.4:0.15:1, genipin aqueous solution Accounting for 1wt% of the total mass of the mixed solution A;

[0030] (5) Disperse the collagen / keratin / hydro...

Embodiment 2

[0033] A preparation method of collagen-hydroxyapatite artificial bone, specifically comprising the following steps:

[0034] (1) Polylactic acid was prepared into a 3wt% solution with tetrahydrofuran;

[0035] (2) Prepare chitosan, and prepare the prepared chitosan into a 0.05wt% solution with dilute acetic acid;

[0036] (3) Prepare a collagen solution with a concentration of 12g / L and a keratin solution with a concentration of 5g / L with distilled water;

[0037] (4) Add hydroxyapatite to the mixed solution of 12g / L collagen and 5g / L keratin, adjust the pH to 7.0 with sodium hydroxide solution, and add 1.5wt% genipin aqueous solution at 4 Cross-linking at ℃ for 2 hours to obtain mixed solution A, freeze-drying to obtain collagen / keratin / hydroxyapatite composite powder with a mass ratio of collagen, keratin and hydroxyapatite of 0.6:0.25:1, genipin aqueous solution Accounting for 1.5wt% of the total mass of the mixed solution A;

[0038] (5) Disperse the collagen / keratin / h...

Embodiment 3

[0041] A preparation method of collagen-hydroxyapatite artificial bone, specifically comprising the following steps:

[0042] (1) Polylactic acid was prepared into a 2wt% solution with tetrahydrofuran;

[0043] (2) Prepare chitosan, and prepare the prepared chitosan into a 0.03wt% solution with dilute acetic acid;

[0044] (3) Prepare a collagen solution with a concentration of 10g / L and a keratin solution with a concentration of 3g / L with distilled water;

[0045] (4) Add hydroxyapatite to the mixed solution of 10g / L collagen and 3g / L keratin, adjust the pH to 7.0 with sodium hydroxide solution, and add 1.5wt% genipin aqueous solution at 4 Cross-linking at ℃ for 2 hours to obtain mixed solution A, freeze-drying to obtain collagen / keratin / hydroxyapatite composite powder with a mass ratio of collagen, keratin and hydroxyapatite of 0.5:0.2:1, genipin aqueous solution Accounting for 1.2wt% of the total mass of the mixed solution A;

[0046] (5) Disperse the collagen / keratin / hy...

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Abstract

The invention discloses a preparation method of collagen-hydroxyapatite artificial bone. The method comprises the following steps: adding hydroxyapatite into a mixed solution of collagen and keratin; adjusting the pH to 7.0 with a NaOH solution; adding a genipin aqueous solution for crosslinking; performing freeze drying, and dispersing the obtained collagen / keratin / hydroxyapatite composite powder into a chitosan solution; adding a collagen solution and a keratin solution again and adding a genipin aqueous solution for crosslinking, and performing freeze drying to obtain composite powder B; adding the composite powder B and nano cellulose into a polylactic acid solution; and stirring and blending and performing freeze drying and grinding to obtain collagen-hydroxyapatite artificial bone. In the invention, the prepared collagen-hydroxyapatite artificial bone has good mechanical property, degradability and biocompatibility and can better promote the adhesion and multiplication of mesenchymal stem cells, and provides a space structure and environment more suitable for the growth of mesenchymal stem cells.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a preparation method of collagen-hydroxyapatite artificial bone. Background technique [0002] Bone defects are one of the common diseases in orthopedic surgery, head and neck surgery, plastic surgery, etc. They will not only cause serious obstacles to the physiological functions of patients, but also seriously affect the appearance and image of patients, causing heavy mental burden to patients. At present, clinical work Bone grafting is often used for repair. Therefore, scholars at home and abroad have studied many bone substitute materials. However, due to various defects, these materials cannot fully meet the requirements of clinical repair. [0003] Biocompatibility and degradability are important indicators to measure whether bone repair materials are excellent. Chitosan is a natural biological material with a positive charge. Its structure is very similar ...

Claims

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Application Information

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IPC IPC(8): A61L27/54A61L27/22A61L27/20A61L27/18A61L27/12C08B37/08
CPCA61L27/18A61L27/12A61L27/20A61L27/227A61L27/54A61L2430/02C08B37/0003C08B37/003C08L67/04C08L5/08C08L89/00
Inventor 张其清张瑗袁平林芬香林强
Owner 福州市大福瑞生物科技有限公司
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