Strain of Dongxiang wild rice endophytic fungus for producing GAMG (glycyrrhetinic acid mono-glucuronide) by efficiently converting glycyrrhizic acid and application thereof

A technology of endophytic fungi and glycyrrhizic acid, applied in the direction of fungi, microorganism-based methods, microorganisms, etc., can solve many problems such as low energy consumption, high yield, and simple operation

Active Publication Date: 2017-05-24
JIANGXI SCI & TECH NORMAL UNIV
View PDF2 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to a series of related research reports, the strains used for biotransformation and synthesis of GAMG are mainly concentrated

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Strain of Dongxiang wild rice endophytic fungus for producing GAMG (glycyrrhetinic acid mono-glucuronide) by efficiently converting glycyrrhizic acid and application thereof
  • Strain of Dongxiang wild rice endophytic fungus for producing GAMG (glycyrrhetinic acid mono-glucuronide) by efficiently converting glycyrrhizic acid and application thereof
  • Strain of Dongxiang wild rice endophytic fungus for producing GAMG (glycyrrhetinic acid mono-glucuronide) by efficiently converting glycyrrhizic acid and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The separation of Chaetomium globosum DX-THS3 of the present invention:

[0028] (1) Collect complete Dongxiang wild rice and return it to the laboratory for immediate processing. Roots, stems and leaves are all soaked in 75% alcohol for 5 minutes for preliminary sterilization, then soaked in 0.1% mercury chloride for 8 minutes, and then rinsed with sterile water to sterilize the surface of the tissue.

[0029] (2) The above-mentioned treated stems are all subtracted with sterilized scissors, and then the stems with the two ends removed are cut longitudinally, divided into two, and then cut into small pieces of 0.1cm×0.5cm. They were respectively placed on PDA medium supplemented with 60 μg / L streptomycin and 0.5 g / L potassium dichromate, and cultured in an incubator at 28°C. In order to check the effect of surface sterilization, a control group was set up to test the effect of sterilization. Control Ⅰ: The sterilized roots, stems and leaves are not subtracted from th...

Embodiment 2

[0033] Screening of Chaetomium globosum DX-THS3 of the present invention:

[0034] (1) Plate initial screening: Activate the Dongxiang wild rice endophytic fungus obtained from the above separation and purification, and then inoculate it into a solid screening medium with glycyrrhizic acid (glycyrrhizinate or licorice extract) as the only carbon source, and the control group uses glucose as the only carbon source. Placed in an incubator at 28°C, cultured continuously for 7 days, and screened out the strains that can grow well on the medium with glycyrrhizic acid as the only carbon source.

[0035] The composition and proportioning of the solid screening medium are: ammonium glycyrrhizinate 1.0g / L, KH 2 PO 4 2.2g / L, NH 4 NO 3 3.0g / L, NaCl 0.5g / L, yeast powder 0.05g / L, MgSO 4 ·7H 2 O 0.25g / L, CaCl 2 0.011g / L, ZnSO 4 ·7H 2 O2.87mg / L, MnSO 4 ·H 2 O 1.69mg / L, H 3 BO 3 0.03mg / L, agar powder 20g / L, pure water 1000mL, adjust the pH to 5.0-7.0, and sterilize under high-...

Embodiment 3

[0041] Transformation of licorice extract by Chaetomium globosum DX-THS3 to produce GAMG:

[0042] Inoculate the activated Chaetomium globosum DX-THS3 into the seed medium, place it in a shaker with a temperature of 28°C and a rotation speed of 120r / min, and cultivate it continuously for 3 days, according to 1-10% (w / Inoculation amount of v) Inoculate the above-mentioned cultivated seeds into the transformation medium containing glycyrrhizic acid, place them in a shaker at a temperature of 28° C. and a rotation speed of 120 r / min, and cultivate continuously for 7 days. The bacterial cells were removed by centrifugation, the fermentation broth was collected, and a 0.22 μm microporous filter head was used to detect the transformation product by HPLC.

[0043] The composition and proportioning of described seed culture medium are: glucose 20g / L, KH 2 PO 4 1.2~3.2g / L, NH 4 NO 3 2.0~5.0g / L, NaCl 0.5~1.0g / L, yeast powder 0.05~0.3g / L, MgSO 4 ·7H 2 O 0.25~0.5g / L, CaCl 2 0.01...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a strain of a Dongxiang wild rice endophytic fungus for producing GAMG (glycyrrhetinic acid mono-glucuronide) by efficiently converting glycyrrhizic acid. The classification and nomenclature of the Dongxiang wild rice endophytic fungus is Chaetomium globosum DX-THS3, is collected in CCTCC (China Center for Type Culture Collection) and has the preservation number of CCTCC NO: M 2016005. By using the glycyrrhizic acid (glycyrrhetate or coarse liquorice root extracts) as carbon sources; the strain can be induced to generate beta-D-glucuronidase; further, the remote end glucuronyl in glycyrrhizic acid molecules is hydrolyzed; the GAMG is produced. The Chaetomium globosum DX-THS3 and the produced beta-D-glucuronidase can be used for producing the GAMG by converting the glycyrrhizic acid. The characteristics of single products, high conversion rate and the like are realized.

Description

technical field [0001] The invention relates to the field of biotransformation, in particular to a Dongxiang wild rice endophytic fungus capable of efficiently transforming glycyrrhizic acid to produce GAMG and its application method in transforming glycyrrhizic acid to produce GAMG. Background technique [0002] Glycyrrhizic acid (Glycyrrhizic acid, GL), also known as glycyrrhizin, is one of the main active ingredients in medicinal licorice. Glycyrrhizin is composed of one molecule of pentacyclic triterpenoid saponins connected with two molecules of glucuronic acid through glycosidic bonds. After glycyrrhizic acid is hydrolyzed by β-D-glucuronidase, a distal glucuronic acid group is removed to generate monoglucuronic acid glycyrrhetinic acid (GAMG). [0003] Glycyrrhetinic acid mono-Glucuronide (GAMG), also known as glycyrrhetin, is a natural sweetener, which not only has the excellent characteristics of high sweetness and low calorie, but also increases the raw Some flav...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/14C12P19/56C12R1/645
CPCC12P19/56C12N1/145C12R2001/645
Inventor 朱笃崔琼琼肖依文吴文婷汪涯常军张志斌
Owner JIANGXI SCI & TECH NORMAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap