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Process for producing biological packing bacterium bacillus natto

A technology for the production of Bacillus natto, which is applied in the field of production technology of the biological bedding bacterium Bacillus natto, can solve the problems of high production cost, low spore rate, and long time consumption of Bacillus natto, and achieve low cost, The effect of short time consumption and great economic value

Active Publication Date: 2017-05-24
上海国龙生物技术集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, the production cost of Bacillus natto is high, time-consuming, and the spore rate is low. The present invention aims to provide a production process for the biological bedding bacterium Bacillus natto, which has the advantages of short time consumption, low cost, and high spore rate. It has great economic value and has broad market prospects in the field of agriculture and breeding

Method used

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  • Process for producing biological packing bacterium bacillus natto

Examples

Experimental program
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Effect test

Embodiment 1

[0033] The strain used in the examples of the present invention was purchased from Shaoguan Yilinquan Biotechnology Co., Ltd., and is Bacillus natto for common feed.

[0034] A production process of the biological bedding bacteria Bacillus natto, the steps are as follows:

[0035] (1) Preparation of seed culture medium:

[0036] The formula is as follows: glucose 50g / L, yeast powder 8g / L, peptone 8g / L, beef extract 3g / L, plant compound fermentation extract 5g / L, ammonium sulfate 0.05g / L, NaCl 0.5g / L, dihydrogen phosphate Sodium 3g / L, magnesium sulfate 0.02g / L;

[0037] The preparation method of the plant compound extract is as follows:

[0038] According to parts by weight, weigh 10 parts of Tribulus terrestris, 8 parts of rose, 2 parts of sea coconut, 1 part of Houttuynia cordata, add 100 parts of deionized water, keep boiled for 20 minutes, then stand for 24 hours, and filter with 200 mesh filter cloth. Obtain extract and medicine residue;

[0039] Add the obtained medicine residue a...

Embodiment 2

[0053] A production process of the biological bedding bacteria Bacillus natto, the steps are as follows:

[0054] (1) Preparation of seed culture medium:

[0055] The formula is as follows: glucose 50g / L, yeast powder 8g / L, peptone 8g / L, beef extract 3g / L, plant compound fermentation extract 6g / L, ammonium sulfate 0.05g / L, NaCl 0.5g / L, dihydrogen phosphate Sodium 3g / L, magnesium sulfate 0.02g / L;

[0056] The preparation method of the plant compound extract is as follows:

[0057] According to parts by weight, weigh 12 parts of Tribulus terrestris, 5 parts of rose, 2 parts of sea coconut, 1 part of Houttuynia cordata, add 100 parts of deionized water, keep boiling for 20 minutes, then let stand for 24 hours, and filter with 200 mesh filter cloth. Obtain extract and medicine residue;

[0058] Add the obtained medicine residue and deionized water to deionized water in a weight-volume ratio of 1:5, boil again and keep boiling for 50 minutes, and filter to obtain a filtrate;

[0059] After ...

Embodiment 3

[0072] A production process of the biological bedding bacteria Bacillus natto, the steps are as follows:

[0073] (1) Preparation of seed culture medium:

[0074] The formula is as follows: glucose 50g / L, yeast powder 8g / L, peptone 8g / L, beef extract 3g / L, plant compound fermentation extract 7g / L, ammonium sulfate 0.05g / L, NaCl 0.5g / L, dihydrogen phosphate Sodium 3g / L, magnesium sulfate 0.02g / L;

[0075] The preparation method of the plant compound extract is as follows:

[0076] According to parts by weight, weigh 14 parts of Tribulus terrestris, 5 parts of rose, 2 parts of sea coconut, 1 part of Houttuynia cordata, add 100 parts of deionized water, keep boiling for 20 minutes, then let stand for 24 hours, and filter with 200 mesh filter cloth. Obtain extract and medicine residue;

[0077] Add the obtained medicine residue and deionized water to deionized water in a weight-volume ratio of 1:5, boil again and keep boiling for 50 minutes, and filter to obtain a filtrate;

[0078] After ...

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Abstract

The invention discloses a process for producing biological packing bacterium bacillus natto. The process comprises the following steps: (1) preparing a seed culture medium; (2) culturing seeds; (3) preparing a fermenting culture medium from glucose, bran, soybean meal, NaCl, stachyose, monopotassium phosphate, calcium chloride and plant compound fermented extract with pH value of 7.5; and (4) culturing by fermentation, namely culturing for 18-20 hours at low temperature of 32-35 DEG C with the inoculum size of 4 percent (v / v) and liquid measure of 75 percent and sufficiently supplying oxygen during the quick fermenting stage; adding glucose twice in the quick fermenting stage, and controlling the final concentration is not higher than 20 percent; and adding ailanthus ketone, reducing temperature to 10-15 DEG C, and performing anaerobic fermentation for 2-3 hours to improve the spore rate. The process has the advantages of short time consumption, low cost and high spore rate, has a great economic value, and has wide market prospect in the field of agriculture breeding.

Description

Technical field [0001] The invention relates to a production process of a biological bedding bacterium Bacillus natto, which belongs to the field of microorganism cultivation. Background technique [0002] Fermentation bed pig raising is a special flora preparation containing one or more beneficial microorganisms such as Bacillus natto, Bacillus subtilis, Actinobacillus, Lactobacillus, etc., mixed with sawdust, rice husk, straw and other bedding materials to form a fermentation bed , Beneficial bacteria multiply through aerobic and anaerobic fermentation to form a dominant flora. The pigs raised on the fermentation bed excrete the feces and urine into the fermentation bed, through the pig arch or artificial turning, the feces and the litter are fully stirred and mixed evenly, and the feces are quickly degraded and digested by the beneficial bacteria in the litter, without odor and no smell. Pollution, so as to achieve the goal of zero discharge of feces and urine, energy saving ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N3/00C12N1/20C12R1/07
CPCC12N1/20C12N3/00
Inventor 雷雨吕晓平姜蒙钟诚
Owner 上海国龙生物技术集团有限公司
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