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Method for increasing content of mogrol in momordica grosvenori

A technology of Luo Han Guo alcohol and Luo Han Guo, which is applied in the field of plant biology, can solve the problems of restricting the healthy development of the Luo Han Guo industry and low content, and achieve the effects of avoiding coumarin precipitation, increasing the content, and promoting the activity level

Inactive Publication Date: 2017-05-31
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have found that mogrosanol is an intermediate product in the biosynthesis of glucoside V. In the late development of Luo Han Guo fruit, mogrosanol is completely consumed and transformed into glucoside V. However, mogrosanol only exists in the pulp accounting for less than 15% of the fruit weight. And the content is extremely low, seriously restricting the healthy development of Luo Han Guo industry
In the prior art, there is no report that methyl jasmonate improves mogrosanol content

Method used

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  • Method for increasing content of mogrol in momordica grosvenori

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Step 1. Dissolve methyl jasmonate with an aqueous ethanol solution with a volume fraction of 2%, prepare an ethanol mother liquor with a concentration of 200 mmol / L methyl jasmonate, and sterilize it with a 0.22 μm microporous filter. The ethanol mother liquor of methyl jasmonate was added to the MS solid medium, the amount of methyl jasmonate was 200 μmol / L, sterilized and cooled to 24-26 ° C, wherein the MS solid medium included: MS, 1.5 mg / L L 6-benzylaminoadenine, 0.3mg / L indole butyric acid, 3.5g / L agar, 30g / L sucrose, and 1.0g / L activated carbon;

[0028] Step 2: Inoculate the Luo Han Guo tissue culture seedlings in the MS solid medium containing methyl jasmonate obtained in step 1, place the relative humidity at 60-66%, the light intensity of 1300-1500 Lux, the light time of 8h / d, and the temperature of 21~ Cultivated at 25°C for 30 d, and then transplanted the tissue culture seedlings to the field for routine field cultivation.

Embodiment 2

[0030] Dissolve methyl jasmonate with an aqueous ethanol solution with a volume fraction of 2%, and prepare a methyl jasmonate solution with a concentration of 200 μmol / L. After 25 days of Luo Han Guo pollination, spray the methyl jasmonate solution on the surface of Luo Han Guo every day until the surface drips. Spray once in the morning, at noon and in the evening, and spray continuously for 10 days.

Embodiment 3

[0032] Step 1. Dissolve methyl jasmonate with an aqueous ethanol solution with a volume fraction of 2%, prepare an ethanol mother liquor with a concentration of 50 mmol / L methyl jasmonate, and sterilize it with a 0.22 μm microporous filter. The ethanol mother liquor of methyl jasmonate was added to the MS solid medium to the amount of methyl jasmonate to be 50 μmol / L, sterilized and cooled to 24-26 ° C, wherein the MS solid medium included: MS, 1.5 mg / L L 6-benzylaminoadenine, 0.3mg / L indole butyric acid, 3.5g / L agar, 30g / L sucrose, and 1.0g / L activated carbon;

[0033] Step 2: Inoculate the Luo Han Guo tissue culture seedlings in the MS solid medium containing methyl jasmonate obtained in step 1, place the relative humidity at 60-66%, the light intensity of 1300-1500 Lux, the light time of 8h / d, and the temperature of 21~ Cultured at 25°C for 28 days;

[0034] Step 3: Dissolve methyl jasmonate with an aqueous ethanol solution with a volume fraction of 2%, prepare a methyl ja...

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Abstract

The invention discloses a method for increasing the content of mogrol in momordica grosvenori. The method is characterized in that jasmonic acid methyl ester is added in the tissue culture process or planting process of the momordica grosvenori. The method is capable of effectively increasing the yield of the mogrol, has the characteristics of being rapid in effect taking, low in cost, simple to operate, convenient to implement, free of toxin or residue in products and the like, is capable of meeting large-scale production of the mogrol, and has relatively good practicability and high popularization values.

Description

technical field [0001] The present invention relates to the field of plant biotechnology. More specifically, the present invention relates to a method for increasing the content of mogrosideol in Luo Han Guo. Background technique [0002] Luo Han Guo (Siraitia grosvenorii) is a precious medicinal and sweet plant unique to China. Its fruit is cool and sweet in taste, and has the functions of clearing away heat and moistening the lungs, soothing the throat and opening the sound, smoothing the intestines, laxative and anti-cancer. It is 300-400 times higher than the degree of sugar and is widely used in food, health products and medicines. It is an ideal sugar substitute for diabetics, obese and hypertensive patients. The study found that mogrosideol is an intermediate product in the biosynthesis of glucoside V. In the late stage of fruit development, mogroside is completely consumed and converted into glucoside V. However, mogroside only exists in the pulp, which accounts fo...

Claims

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Application Information

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IPC IPC(8): A01N65/20A01N61/00A01N43/38A01N43/16A01N43/12A01N37/42A01N37/10A01P3/00A01P17/00A01P21/00A01H4/00A01G1/00
CPCA01N65/00A01G22/00A01H4/001A01H4/008A01N37/10A01N37/42A01N43/12A01N43/16A01N43/38A01N61/00A01N65/20A01N2300/00
Inventor 韦荣昌
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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