Method for increasing content of mogrol in momordica grosvenori
A technology of Luo Han Guo alcohol and Luo Han Guo, which is applied in the field of plant biology, can solve the problems of restricting the healthy development of the Luo Han Guo industry and low content, and achieve the effects of avoiding coumarin precipitation, increasing the content, and promoting the activity level
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Embodiment 1
[0027] Step 1. Dissolve methyl jasmonate with an aqueous ethanol solution with a volume fraction of 2%, prepare an ethanol mother liquor with a concentration of 200 mmol / L methyl jasmonate, and sterilize it with a 0.22 μm microporous filter. The ethanol mother liquor of methyl jasmonate was added to the MS solid medium, the amount of methyl jasmonate was 200 μmol / L, sterilized and cooled to 24-26 ° C, wherein the MS solid medium included: MS, 1.5 mg / L L 6-benzylaminoadenine, 0.3mg / L indole butyric acid, 3.5g / L agar, 30g / L sucrose, and 1.0g / L activated carbon;
[0028] Step 2: Inoculate the Luo Han Guo tissue culture seedlings in the MS solid medium containing methyl jasmonate obtained in step 1, place the relative humidity at 60-66%, the light intensity of 1300-1500 Lux, the light time of 8h / d, and the temperature of 21~ Cultivated at 25°C for 30 d, and then transplanted the tissue culture seedlings to the field for routine field cultivation.
Embodiment 2
[0030] Dissolve methyl jasmonate with an aqueous ethanol solution with a volume fraction of 2%, and prepare a methyl jasmonate solution with a concentration of 200 μmol / L. After 25 days of Luo Han Guo pollination, spray the methyl jasmonate solution on the surface of Luo Han Guo every day until the surface drips. Spray once in the morning, at noon and in the evening, and spray continuously for 10 days.
Embodiment 3
[0032] Step 1. Dissolve methyl jasmonate with an aqueous ethanol solution with a volume fraction of 2%, prepare an ethanol mother liquor with a concentration of 50 mmol / L methyl jasmonate, and sterilize it with a 0.22 μm microporous filter. The ethanol mother liquor of methyl jasmonate was added to the MS solid medium to the amount of methyl jasmonate to be 50 μmol / L, sterilized and cooled to 24-26 ° C, wherein the MS solid medium included: MS, 1.5 mg / L L 6-benzylaminoadenine, 0.3mg / L indole butyric acid, 3.5g / L agar, 30g / L sucrose, and 1.0g / L activated carbon;
[0033] Step 2: Inoculate the Luo Han Guo tissue culture seedlings in the MS solid medium containing methyl jasmonate obtained in step 1, place the relative humidity at 60-66%, the light intensity of 1300-1500 Lux, the light time of 8h / d, and the temperature of 21~ Cultured at 25°C for 28 days;
[0034] Step 3: Dissolve methyl jasmonate with an aqueous ethanol solution with a volume fraction of 2%, prepare a methyl ja...
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