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A high protein-producing Chlorella and its culture method and application

A culture method, chlorella technology, applied in the direction of microorganism-based methods, applications, chemical instruments and methods, etc., can solve the problems of protein difficult to be utilized by organisms, algae cell wall thickness, low protein production, etc., to achieve favorable absorption and utilization , High protein content, thin cell wall effect

Active Publication Date: 2019-07-26
山东宝来利来生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are relatively few studies on the use of microalgae for waste water treatment and as food and bioenergy, and most of them are in the laboratory test stage of algae screening. High condition requirements, low protein yield, and relatively thick algae cell walls make it difficult for proteins to be utilized by organisms, making it difficult to achieve wide application

Method used

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  • A high protein-producing Chlorella and its culture method and application
  • A high protein-producing Chlorella and its culture method and application
  • A high protein-producing Chlorella and its culture method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Isolation, screening and identification of algal strains

[0022] 1 sample collection

[0023] Chlorella isolation sample collection location: cesspool and manure discharge ditch of Ningyang pig farm in Taian, Shandong.

[0024] 2 Separation and screening of algae strains

[0025] 2.1 Test material: The culture medium used in this embodiment is Zhu's No. 10 medium, and 1% (mass percentage) of agar is added to its solid medium, and no agar is added to its liquid medium. The composition of the medium is shown in Table 1.

[0026] Table 1 Composition of Zhushi No. 10 Medium

[0027]

[0028]

[0029] 2.2 Test method

[0030] 2.2.1 Place the collected samples in a light incubator at 30°C for 5-7 days. After turning green, carry out 10, 50, 100, 500 and 1000-fold gradient dilution with Zhushi No. 10 liquid medium, and take After dilution, 100 μL of the sample was evenly spread on Zhushi No. 10 solid medium, and placed in a light incubator at 30°C for cu...

Embodiment 2

[0096] Embodiment 2: the application in the production protein of chlorella of the present invention

[0097] 1 Determination of the amount of algae powder required for the determination of protein content

[0098] 1.1 Test material

[0099] Algae strain: Chlorella sorokiniana BL-ch1 obtained by screening and separating in Example 1 of the present invention;

[0100] Medium: Zhushi No. 10, formula: 0.1g sodium nitrate, 0.01g dipotassium hydrogen phosphate, 0.25g magnesium sulfate, 0.02g sodium carbonate, 0.025g sodium silicate, 0.008g ferric chloride, 1L of water.

[0101] 1.2 Test method

[0102] Biomass detection --- weighing bottle method;

[0103] Protein content detection --- Kjeldahl method.

[0104] 1.3 Test steps

[0105] Prepare Zhu’s No. 10 medium, inoculate the algae strain BL-ch1 into the medium, culture at 33°C for 5 days, centrifuge the algae liquid at 5000r / min for 5min, concentrate the obtained algae mud, wash it three times with pure water, and then wash ...

Embodiment 3

[0179] Embodiment 3: Application of Chlorella of the present invention in degrading feces and sewage

[0180] 1 Experimental design

[0181] The experimental design is shown in Table 21.

[0182] Table 21 Design of Chlorella Degradation Fecal Sewage Test

[0183]

[0184]

[0185] The cultivation method of the algae liquid: the algae strain is the algae strain BL-ch1 screened in Example 1 of the present invention, the inoculum size is 10% (volume fraction), the liquid volume is 600mL / L, the initial pH value is 7.0, and the temperature is 28°C-33°C .

[0186] Considering the smell of feces and the convenience of sampling, this experiment adopted a sorting box with a cover, and used a 3L triangular flask to compare the difference in illuminance, as shown in image 3 shown.

[0187] 2 Experimental results

[0188] 2.1 Degradation of total nitrogen (total nitrogen content mg / L)

[0189] The results are shown in Table 22.

[0190] Table 22 Degradation Results of Total ...

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Abstract

The present invention discloses a strain of Chlorella, named as Chlorella sorokiniana BL‑ch1, which has been preserved in the China Typical Culture Collection Center on November 8, 2016, address: Luojia Mountain, Wuchang City, Wuhan City, its The deposit number is: CCTCC M 2016626. The cultivation method of the algae strain is as follows: the algae strain is inoculated on the culture medium, the inoculation amount is 5-15%, the liquid filling volume is 400-800mL / L, the initial pH is 6.0-8.0, and the cultivation temperature is 28-33°C. The chlorella screened by the invention has high protein content and high degradation rate of total nitrogen, total phosphorus and ammonia nitrogen, and can be used in multiple fields such as protein production, feed preparation, sewage treatment and the like.

Description

technical field [0001] The invention relates to a strain of chlorella with high protein yield and its cultivation method and application, belonging to the field of algae biotechnology. Background technique [0002] Microalgae is a kind of microorganism capable of photosynthetic autotrophy. It is a primary producer in the ecosystem and plays a huge role in the entire material cycle. It has high production efficiency and attractive development prospects. Microalgae will absorb and utilize a large amount of nitrogen and phosphorus elements during the growth process, so the post-treatment unit of the sewage treatment plant can be used to cultivate microalgae for denitrification and phosphorus removal and deep purification of sewage. As early as 1957, Oswald et al. suggested that microalgae should be used as A biological system that replaces activated sludge in sewage treatment. Since then, the oxidation pond technology based on the principle of symbiosis and self-purification o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C02F3/32A23K10/18A23K50/80C12R1/89
CPCC02F3/322C12N1/12C12N1/125C12R2001/89
Inventor 单宝龙陈雷王春凤谷巍陈玉春姜延龙王红赵倩高绪娜郭婷婷李光
Owner 山东宝来利来生物工程股份有限公司
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