Method for improving salt resistance of alfalfa
An alfalfa and salt-tolerant technology, which is applied in the direction of soil preparation methods, botany equipment and methods, ammonium salt fertilizer, etc., can solve the problems of poor stable development of animal husbandry, restrictions on the development of alfalfa industry, inhibition of alfalfa growth, etc., and achieve an increase in relative growth rate , reduce malondialdehyde and soluble protein content, improve the effect of salt tolerance
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Embodiment 1
[0038] The application of betaine in artificially controlled environment in greenhouse improves the salt tolerance of alfalfa, the main steps are as follows:
[0039] 1. Alfalfa planting
[0040] Alfalfa variety 'Salt Tolerant Star' was selected as material, sown in plastic cups with fine sand (upper diameter 6.8 cm, lower diameter 4.8 cm, height 7.4 cm), covered with sand 1 cm after sowing, covered with three layers of toilet paper, Pour 1 / 2 Hoagland nutrient solution, keep it moist until seedlings emerge, and place them in an artificially controllable greenhouse for cultivation. The temperature is 25°C during the day, 20°C at night, the light time is 16h, the humidity is 50%, and the light intensity is 600μmol / m 2 ·s.
[0041] 2. Determination of the optimal application concentration of betaine
[0042] When the alfalfa grows to the seedling age of 45 days, take out the alfalfa, wash the sand, and transfer it to a 250mL Erlenmeyer flask. The triangular flask is filled wit...
Embodiment 2
[0045] The application of betaine in the natural environment of the field improves the salt tolerance of alfalfa, and the operation steps are as follows:
[0046] Choose an alfalfa variety with strong salt tolerance - Salt Tolerant Star. Dig drainage ditches around the plot to ensure smooth drainage of the plot. Plow the soil more than 35cm deep, and apply wheat straw (at 3.0kg / m 2 count). Apply 1500kg of organic fertilizer (cow dung) per mu, 10kg of urea, 75kg of superphosphate, 30kg of potassium sulfate, 4kg of magnesium sulfate, 1.0kg of zinc sulfate monohydrate, 1.0kg of borax, 7.5g of ammonium molybdate, 10cm of rotary tillage, rake in time flat;
[0047] On August 25, the seeds were artificially removed from impurities and mixed with rhizobia agent (powder, 5g rhizobium per kilogram of seed dosage) for seed dressing. On the same day, the seeds were sown and drilled. The sowing rate was 1.2kg per mu, and the row spacing was 30cm. Depth 2cm;
[0048] On the 3rd day af...
Embodiment 3
[0051] The application of betaine in the natural environment of the field improves the salt tolerance of alfalfa, and the operation steps are as follows:
[0052]Alfalfa variety Zhongmi No.1 with strong salt tolerance was selected. Dig drainage ditches around the plot to ensure smooth drainage of the plot. Plow the soil more than 35cm deep, and apply rice straw (at 3.0kg / m 2 count). Apply 1500kg of organic fertilizer (pig manure) per mu, 10kg of urea, 75kg of superphosphate, 30kg of potassium sulfate, 4kg of magnesium sulfate, 1.0kg of zinc sulfate monohydrate, 1.0kg of borax, 7.5g of ammonium molybdate, 10cm of rotary tillage, and rake in time flat;
[0053] On August 25, the seeds were artificially removed from impurities and mixed with rhizobia agent (powder, 5g rhizobium per kilogram of seed dosage) for seed dressing. On the same day, the seeds were sown and drilled. The sowing rate was 1.2kg per mu, and the row spacing was 30cm. Depth 2cm;
[0054] On the 3rd day aft...
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