Lactobacillus brevis DM9218 capable of reducing blood uric acid, gene segment and recombinant protein

A technology of DM9218, Lactobacillus brevis, applied in DNA/RNA fragments, genetic engineering, plant genetic improvement, etc., can solve the problem of low decomposition rate and decomposition rate of purine nucleosides, difficulty in diet control, and can not meet the clinical requirements for reducing blood uric acid. and other problems, to achieve the effect of non-toxic side effects

Inactive Publication Date: 2017-06-13
DALIAN MEDICAL UNIVERSITY
View PDF1 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the content of purines in various foods has not been determined accurately, especially the umami flavor agents such as chicken essence, oyster sauce, and fish sauce that improve the flavor of food, the main component of which is 5'-taste nucleotide disodium (including 5' '-guanylate disodium and 5'-inosinate disodium) are purine substances, so strict dietary control is extremely difficult
[0003] Intestinal lactic acid bacteria are beneficial bacteria in the gastrointestinal tract of the body. Although it has been confirmed that they have the function of absorbing purine, purine metabolic intermediates inosine, guanosine and uric acid, the decomposition rate and decomposition rate of purine nucleosides by existing strains All were low, unable to meet the clinical requirements for lowering blood uric acid

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lactobacillus brevis DM9218 capable of reducing blood uric acid, gene segment and recombinant protein
  • Lactobacillus brevis DM9218 capable of reducing blood uric acid, gene segment and recombinant protein
  • Lactobacillus brevis DM9218 capable of reducing blood uric acid, gene segment and recombinant protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The salted Chinese cabbage (sauerkraut) fermentation liquid was cultured with lactic acid bacteria selection medium, and then anaerobically cultured at 37°C. Lactobacillus brevis DM9218 was isolated according to conventional methods. No.: CGMCC No. 12797.

[0026] experiment:

[0027] 1. Verification of the ability of Lactobacillus brevis DM9218 to decompose purine nucleosides

[0028] Lactobacillus brevis DM9218 was inoculated in MRS medium for anaerobic culture for 48 hours, the cells were collected by centrifugation, and neutral phosphate buffer containing inosine and guanosine was added to the cells. Under anaerobic conditions at 37°C, shake culture at 120r / min for 60min, then centrifuge to take the supernatant and add 0.1mol / l HClO at a ratio of 9:1 4 solution for RP-HPLC analysis. Chromatographic conditions: high performance liquid chromatography: LC-20A; reversed phase liquid chromatography column: Cosmosil-5C 18 -AR- ;Mobile phase: 0.1μmol / l NaClO 4 –0.1...

Embodiment 2

[0051] Whole genome sequencing of Lactobacillus breve strain DM9218.

[0052]The activated Lactobacillus brevis DM9218 was cultivated, collected by centrifugation, washed, broken by lysozyme, separated and purified to obtain bacterial genomic DNA. Entrusted Shanghai Passino Biotechnology Co., Ltd. to determine the whole genome of DM9218, the DNA sequence is shown in SEQ ID NO:1.

Embodiment 3

[0054] According to the whole genome sequence analysis of Lactobacillus brevis DM9218, gene A0008 encodes inosine hydrolase, which can participate in the decomposition of inosine. The DNA sequence of gene A0008 is shown in SEQ ID NO:2.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
wavelengthaaaaaaaaaa
Login to view more

Abstract

The invention discloses lactobacillus brevis DM9218 capable of decomposing purine nucleoside with high decomposition rate at high speed and reducing blood uric acid, a gene segment and a recombinant protein. The lactobacillus brevis DM9218 (Lactobacillus brevis) is preserved in China General Microbiological Culture Collection Center with the preservation number being CGMCC NO.12797; the gene segment of the lactobacillus brevis DM9218 has a DNA (Deoxyribonucleic Acid) sequence as shown in SEQ ID NO:2; the recombinant protein of the gene segment of the lactobacillus brevis DM9218 is obtained through adopting a DNA of the lactobacillus brevis DM9218 as a template for PCR (Polymerase Chain Reaction), and utilizing a gene cloning method for a PCR product, wherein DNA sequences of an upstream primer and a downstream primer of PCR reaction are respectively as shown in SEQ ID NO:3 and SEQ ID NO:4.

Description

technical field [0001] The invention belongs to the field of biology, in particular to a Lactobacillus brevis DM9218 capable of reducing blood uric acid, a gene fragment and a recombinant protein with fast decomposing rate and high decomposing rate of purine nucleosides. Background technique [0002] Serum uric acid is not only an index for judging gout, but also an important index for judging blood pressure, blood sugar and blood lipids. Excessive blood uric acid is closely related to lipid metabolism disorders, diabetes, hypertension, obesity and other metabolic diseases . In addition to drug treatment, existing hyperuricemia is generally supplemented by strict diet control, that is, reducing the intake of exogenous purine substances as much as possible. However, since the content of purines in various foods has not been determined accurately, especially the umami flavor agents such as chicken essence, oyster sauce, and fish sauce that improve the flavor of food, the main...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N15/11C12N15/55C12N9/86C12R1/24
CPCC12N9/86C12N15/11C12Y305/0201C12N1/205C12R2001/24
Inventor 袁杰力郑鹏远李明杨殿斌王凤娇
Owner DALIAN MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap