Application of streptomyces pactum to controlling parasitic weeds

A technology of streptomyces and parasiticity, which is applied in the application field of streptomyces in the control of parasitic weeds, and can solve the problems that the streptomyces does not have the ability to prevent and control melons, and achieves inhibition of germination and growth , increase yield, inhibit seed germination

Inactive Publication Date: 2017-07-21
陕西博秦生物工程有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Preliminary test results show that Streptomyces convolvulus has multiple functions of disease prevention and growth promotion, and has a significant inhibitory effect on pathogenic bacteria of soil-borne root diseases.

Method used

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  • Application of streptomyces pactum to controlling parasitic weeds
  • Application of streptomyces pactum to controlling parasitic weeds
  • Application of streptomyces pactum to controlling parasitic weeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Inhibition of Fermentation Broth of Streptomyces clumps on Gualiedang Seed Germination

[0032] 1. Test method:

[0033] (1) Preparation of Streptomyces convolutum fermented liquid

[0034] The fermented liquid of Streptomyces convolutum adopts the mode of known liquid shaker culture to carry out, and stoste (every milliliter of fermented liquid 3.5mg dry mycelia) is diluted 10,10 respectively 2 、10 3 、10 4 Doubling later for later use. The Gaoshi No. 1 liquid medium and the PDA liquid medium that were not inoculated with Streptomyces closus were used as controls, and repeated 3 times.

[0035] (2) Seed germination test

[0036] Firstly, a glass fiber filter paper piece with a diameter of 8mm was placed in a new Petri dish, and then 20μL of fermentation broth, a piece of pre-cultured melon seed sheet and 20μL of GR24 (0.1mg L -1 ) sequentially added to the glass fiber filter paper. Add sterile water and GR24 (0.1mg L -1 ) instead of the treatment of the fermenta...

Embodiment 2

[0047] Inhibition of Methanol Extract of Streptomyces clostrium on Seed Germination of Gualedang

[0048] 1. Test method

[0049] (1) Preparation of methanol extract of Streptomyces clumps

[0050] Weigh 0.050g of Streptomyces convolvulus and place it in a 1.5mL centrifuge tube, then add 1mL of methanol, ultrasonicate for 30min and then 6000rmin -1 Centrifuge for 2min. The supernatant was regarded as the stock solution, diluted 10 and 100 times for use, and repeated 3 times.

[0051] (2) Seed germination test

[0052] Carry out according to above-mentioned test method that fermented liquid suppresses the seed germination of Gua Liedang, replace fermented liquid with thalline methanol extraction liquid, and test repeats 3 times.

[0053] 2. Test results

[0054] See Table 2 for the test results of the methanol extract of Streptomyces clumps inhibiting the germination of Gualedang seeds. The 50.0mg / mL methanol extract of Streptomyces clumps can completely inhibit the germina...

Embodiment 3

[0059] pot experiment

[0060] 1. Test method:

[0061] The pot experiment was conducted from May to September 2014 at the Guyuan Ecological Experimental Station of the Institute of Soil and Water Conservation, Northwest Agriculture and Forestry University, Ningxia Hui Autonomous Region, China.

[0062] According to the results of laboratory experiments, Streptomyces convolutum was used in pot experiments and inoculated in the soil in the form of bacterial agents. The inoculant of Streptomyces convolutum was prepared according to the method of the 2013 foreign literature "Isolation and evaluation of rhizosphere actinomycetes with potential application for biocontrol of Verticillium wilt of cotton" by Xue et al. The number of live spores in the inoculum was 10 11 g -1 . The soil used for potting is black loam. First, mix the soil and fine sand in a volume ratio of 1:1, and then add urea (20mg kg -1 ), calcium superphosphate (50mgkg -1 ), Guariedang seeds (3.4mg kg -1 ) an...

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Abstract

The invention belongs to the field of technologies for controlling parasitic weeds, and particularly discloses application of streptomyces pactum to controlling parasitic weeds. The Streptomyces pactum SG712100 is preserved in the China Center for Type Culture Collection on 7th April, 2009, and a preservation number of the streptomyces pactum is CCTCC M 209066. The application has the advantages that obvious effects of inhibiting germination of seeds of orobanche aegyptiaca can be realized by the streptomyces pactum; the emergence quantities and the emergence rates of the potted orobanche aegyptiaca in harvest periods can be obviously reduced if streptomyces pactum microbial agents are applied, and the total dry weight of the orobanche aegyptiaca and the dry weight of each single plant of the streptomyces pactum in the harvest periods can be obviously decreased; micro-floras in soil can be improved, the total numbers of bacteria in the soil and ratios of the quantities of the bacteria to the quantities of fungi can be increased, the total numbers of the fungi in the soil can be reduced, accordingly, germination and growth of the orobanche aegyptiaca can be inhibited, growth of crops can be promoted, and the yield of the crops can be increased.

Description

technical field [0001] The invention relates to the technical field of parasitic weed control, in particular to the application of Streptomyces convolvulus in the control of parasitic weeds. Background technique [0002] The genus Orobanche spp. includes more than 200 species, many of which are root parasitic weeds of vegetables and crops. Due to the lack of leaves and chloroplasts, Liedang survives entirely by absorbing water and nutrients from the host, thus causing serious damage to the host plant. Melon (Phelipanche aegyptiaca Pers.) belongs to the genus Phelipanche, widely distributed in southern Europe, the Mediterranean region, northern Africa, southern Asia and China, and can be parasitic in Solanaceae, Fabaceae, Umbelliferae and Compositae, etc. crops. [0003] As the damage caused by radang to agricultural production is becoming more and more serious, and radang continues to spread and infect new areas, it is urgent to find measures that can effectively control r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P13/00
CPCA01N63/10
Inventor 陈杰马永清薛泉宏
Owner 陕西博秦生物工程有限公司
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