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Stem-cell skincare composition and preparation and application methods thereof

A skin care composition and stem cell technology, applied in the field of skin care products, can solve the problems of inability to really play a role, cannot penetrate the skin, and have no modification, so as to prevent or improve skin spots, enhance self-metabolism, and delay skin aging. Effect

Active Publication Date: 2017-07-28
杭州哈佛赛尔干细胞技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, because these factors are not modified, they cannot penetrate the skin, and in fact they cannot really play a role.

Method used

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  • Stem-cell skincare composition and preparation and application methods thereof
  • Stem-cell skincare composition and preparation and application methods thereof
  • Stem-cell skincare composition and preparation and application methods thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0058] The method for preparing the above-mentioned stem cell skin care composition includes the following steps:

[0059] Step 1. The stem cell culture medium after culturing the stem cells, the freeze-thaw lysate of the cultured stem cells, serum albumin, mannitol, and Arg9 membrane-permeable peptide are mixed and freeze-dried to obtain a freeze-dried powder;

[0060] Step 2. Mix glycerin, sodium citrate, ellagic acid, vitamin E, and water uniformly to obtain a solvent;

[0061] Step 3. The lyophilized powder prepared in step 1 and the solvent solution obtained in step 2 are packaged separately, and then mixed during use.

[0062] The use method of the above-prepared stem cell skin care composition includes the following steps: mix the freeze-dried powder and the solvent uniformly, shake gently until the freeze-dried powder is completely melted, and then use it; morning and evening. First clean the skin with water or facial cleanser, take an appropriate amount of the product and gen...

Embodiment 1

[0063] Example 1 Using stem cells to express

[0064] 1. Preparation of freeze-dried powder:

[0065] 1.1 Stem cell culture: Inoculate 10 million resuscitated stem cells and 15 ml of stem cell culture medium in a T75 cell culture flask at 5% CO 2 Incubate at 37°C for 48-72 hours. Among them, the stem cell culture medium formula is shown in Table 1.

[0066] Table 1

[0067] Reagent Concentration of stock solution Item No. Final concentration Amount required for 100mL medium KnockOut TM DMEM CTS TM

—A128611X 82.75mL KnockOut TM SR XenoFree CTS TM

—1261815％ 15mL GlutaMAX TM -I CTS TM

200mMA12860 2mM 1mL NEAA 10mM11140 0.1mM 1mL bFGF 10μg / mL13256 8ng / mL 80μL 2-Mercaptoethanol 55mM21985 0.1mM 182μL

[0068] 1.2 Collection and processing: Collect the stem cell culture medium after culturing the stem cells and store it in a sterile container for later use; wash the cultured stem cells twice with sterile saline, add 1 ml trypsin, treat for 5 minutes at 37°C, suck out the...

Embodiment 2

[0072] Example 2 Expression of stem cell factors mediated by plasmid electrotransduction

[0073] 1. Preparation of freeze-dried powder:

[0074] 1.1 The epidermal growth factor (EGF), platelet-derived growth factor (PDGF), transforming growth factor (TGF), basic fibroblast growth factor (b-FGF), insulin-like growth factor (IGF) and nerve growth factor ( NGF), human keratinocyte growth factor (KGF-2), interleukin-1 receptor antagonist (IL-1R) gene 5'ends were fused with the membrane-permeable peptide TAT gene series, and the fused genes were cloned into EGFP. -N1 vector. The vector used for stem cell factor expression is not limited to the EGFP-N1 vector, and includes other vectors that can be expressed in animal cells such as N-P3xflag-CMV, pcDNA3.1, etc.

[0075] 1.2 Electrotransformation of stem cells

[0076] 1.2.1 Preparation of stem cells before transformation: (1) Adherent stem cells are transferred to 75cm 2 The cell culture flask is cultured, and the cell confluence is abou...

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Abstract

The invention discloses a stem-cell skincare composition. The stem-cell skincare composition comprises, by mass, 1-800 parts of component A and a component B, wherein the component A is prepared by stem cell extract, serum albumin, mannitol and an Arg9 cell-penetrating peptides through freeze drying, and the stem cell extract is the freeze-thaw lysate of a stem-cell culture medium after stem cell culture and cultured stem cells; the component B comprises glycerin, sodium citrate, ellagic acid, vitamin E and water; during use, the component A is mixed with the component B. The invention further discloses preparation and application methods of the stem-cell skincare composition. The stem-cell skincare composition has the advantages that various factors of the stem-cell skincare composition have a synergic effect, and the factors can effectively penetrate the skin to enhance the metabolism of the skin from the deep layer, delay skin aging, whiten the skin, prevent or improve skin spots and wrinkles, remove acne and prevent allergy.

Description

Technical field [0001] The present invention relates to the technical field of skin care products, in particular to a stem cell skin care composition and a preparation method and use method thereof. Background technique [0002] With the continuous improvement of living standards, people pay more and more attention to health, especially skin maintenance. Skin tissue is the largest tissue organ of the human body, covering the surface of the human body, blocking the harmful effects of all harmful substances in nature on the living organism. However, with the increase of age, the function of skin tissues and organs gradually ages. From an aesthetic point of view, it seriously affects people's enthusiasm for life; from a physiological point of view, it affects the effective protection of human internal organs. So a variety of beauty and skin care products came into being. [0003] Stem cells are cells with proliferation and differentiation potential, have the ability to self-renew an...

Claims

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Application Information

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IPC IPC(8): A61K8/99A61K8/64A61K8/34A61K8/365A61K8/67A61K8/49A61Q19/08A61Q19/02C07K19/00
CPCA61K8/345A61K8/365A61K8/498A61K8/64A61K8/678A61K8/99A61Q19/02A61Q19/08C07K14/475C07K14/48C07K14/485C07K14/49C07K14/495C07K14/503C07K14/52C07K14/65C07K2319/10
Inventor 范汉东屈彩云
Owner 杭州哈佛赛尔干细胞技术有限公司
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