Method for simultaneously measuring concentrations of six tyrosine kinase inhibitors in blood plasma

A technology of tyrosine kinases and inhibitors, applied in the field of drug analysis, can solve problems such as inconsistent detection standards, and achieve the effects of unification, reduction of subsequent development costs, and enhanced selectivity

Active Publication Date: 2017-07-28
ZHEJIANG CANCER HOSPITAL +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These methods still need to be improved in terms of sensitivity, specificity and h

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  • Method for simultaneously measuring concentrations of six tyrosine kinase inhibitors in blood plasma
  • Method for simultaneously measuring concentrations of six tyrosine kinase inhibitors in blood plasma
  • Method for simultaneously measuring concentrations of six tyrosine kinase inhibitors in blood plasma

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Embodiment Construction

[0035] In order to better illustrate the present invention and understand the technical solutions of the present invention, the present invention will be further described in detail below in conjunction with specific embodiments and accompanying drawings. It should be understood that the following implementation examples are only used to illustrate the present invention, and do not represent or limit the protection scope of the present invention, and the protection scope of the present invention shall be determined by the claims.

[0036] The reagents or instruments used in the following examples are not indicated by the manufacturer, but are all conventional products that can be purchased in the market. For example, apatinib, crizotinib, erlotinib, gefitinib, icotinib, lapatinib, and imatinib are purchased from U.S. Selleck; ethyl acetate, tert-butyl methyl ether, Methanol and formic acid were purchased from Tedia Corporation, USA.

[0037] 1. Preparation of standard curve p...

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Abstract

The invention discloses a method for simultaneously measuring the concentrations of six tyrosine kinase inhibitors in blood plasma. Imatinib is adopted as an internal standard, a medicament in the blood plasma is extracted at first by virtue of a mixed solution of ethyl acetate and tert-butyl methyl ether, then a sample is separated by virtue of high performance liquid chromatography, medicament targeting detection is performed in a high resolution mass spectrometry parallel reaction monitoring mode, and secondary fragment ions of the medicament are used for quantification, so as to realize simultaneous analysis and measurement of the concentrations of the six tyrosine kinase inhibitors in the blood plasma. The method is quick, has the advantages of extremely high targeting performance, high speed, high throughput, high sensitivity, high specificity, high precision and accuracy, high stability, high extraction recovery rate, no obvious substrate effect or dilution effect, and the like, and can be used for plasma concentration monitoring of clinically common antitumor medicaments, i.e. tyrosine kinase inhibitors, and a nanogram-level detection limit can be achieved.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, in particular to a method for simultaneously measuring the concentrations of six tyrosine kinase inhibitors in blood plasma. Background technique [0002] For a long time, cancer has attracted much attention because of its high mortality rate. Current studies have found that cancer is a disease induced by multiple factors, including genome disorder, irregular diet, environmental pollution, radiation and so on. Compared with normal cells, cancer cells have a stronger ability to replicate to resist apoptosis. Clinically, surgery, radiotherapy, chemical drug therapy and targeted drug therapy are mainly used for treatment, among which postoperative chemical drug therapy is one of the commonly used methods. Most chemotherapeutic drugs have poor specificity and usually lead to severe toxic side effects. Therefore, targeted drugs with high specificity are gradually entering the clinic. [0003...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 倪茂巍周洁郑智国许强赵安陈巍
Owner ZHEJIANG CANCER HOSPITAL
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