Method for screening molecular markers relevant to few-root duckweed leaf growth
A technology of molecular markers and leaves, applied in biochemical equipment and methods, determination/inspection of microorganisms, etc., to achieve the effect of improving the efficiency of biological treatment and the detection speed.
Active Publication Date: 2017-08-01
ZHEJIANG OCEAN UNIV
10 Cites 0 Cited by
AI-Extracted Technical Summary
Problems solved by technology
View more
Method used
View more
Abstract
The invention discloses a method for screening molecular markers relevant to few-root duckweed leaf growth. The method comprises the steps of clone body culture, genome DNA (deoxyribonucleic acid) extraction, microsatellite primer design, PCR (polymerase chain reaction) amplification and data statistics and analysis. The method has the beneficial effects that the DNA sequences of a great amount of few-root duckweed marked by the microsatellite primers can be fast obtained; the microsatellite mark detection speed is high; the polymorphic map of the few-root duckweed Sp10, Sp14, Sp42, Sp47, Sp51, Sp52 and Sp53 genetic marker loca showing the high-degree genetic variation can be fast obtained. The leaf length and the leaf width of the few-root duckweed are obviously relevant to Sp10, Sp14, Sp42, Sp47, Sp51, Sp52 and Sp53 (P of the Sp51 site is lower than 0.05, and P of other sites is smaller than 0.001). The influence of the genotype differences on the duckweed growth and pollutant purification capability can be known; the wastewater biological treatment efficiency is improved.
Application Domain
Microbiological testing/measurement
Technology Topic
Genetic markerPollutant +10
Image
Examples
- Experimental program(3)
- Effect test(1)
Example Embodiment
Example Embodiment
Example Embodiment
PUM


Description & Claims & Application Information
We can also present the details of the Description, Claims and Application information to help users get a comprehensive understanding of the technical details of the patent, such as background art, summary of invention, brief description of drawings, description of embodiments, and other original content. On the other hand, users can also determine the specific scope of protection of the technology through the list of claims; as well as understand the changes in the life cycle of the technology with the presentation of the patent timeline. Login to view more.
Similar technology patents
Special device for detecting VL-type universal joint inner star wheel raceway pitch diameter and detection method
Owner:SHANGHAI CHANGRUI AUTO PARTS
FPC flexible plate copper foil oxidation detection method
Owner:SOUTH CHINA UNIV OF TECH
Method and kit for measuring cell activity and application of kit
Owner:INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA +2
Automatic equipment for detecting appearance defects and microporous leakage of ham sausages
Owner:HUNAN ZHENGZHONG PHARMA MACHINERY
Classification and recommendation of technical efficacy words
- detection speed
- Improve bioremediation efficiency
Pedestrian flow monitoring method based on pedestrian detection and tracking
Owner:ZHEJIANG UNIV +1
Hot topic detection method of Chinese micro-blogs
Owner:FUZHOU UNIVERSITY
Method for detecting freshness of mangos by aid of electronic nose
Owner:ZHEJIANG GONGSHANG UNIVERSITY
Fraud website detection method
Owner:CHONGQING UNIV