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Method for screening molecular markers relevant to few-root duckweed leaf growth

A technology of molecular markers and leaves, applied in biochemical equipment and methods, determination/inspection of microorganisms, etc., to achieve the effect of improving the efficiency of biological treatment and the detection speed.

Active Publication Date: 2017-08-01
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The detection method of the invention has the advantages of rapidity, accuracy, sensitivity, etc., and can intuitively detect the genotypes of different individuals of the rusty spot, so as to quickly obtain the polymorphism map of the genetic variation of the functional gene microsatellite locus of the rusty spot, the microsatellite Markers can be applied to the fields of genetic variation and population genetic diversity analysis of rust spot moth, but there is still room for improvement in the detection speed of microsatellite markers

Method used

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  • Method for screening molecular markers relevant to few-root duckweed leaf growth
  • Method for screening molecular markers relevant to few-root duckweed leaf growth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The method for screening the molecular markers related to the growth of the leaves of P. fruticosa includes clone culture, genomic DNA extraction, design of microsatellite primers, PCR amplification, and data statistics and analysis.

[0017] The composition and concentration of the nutrient solution for plant clone culture are: nitrogen 0.3~0.4g / L, phosphoric anhydride 0.1~0.15 g / L, potassium oxide 0.16~0.23 g / L, magnesium oxide 0.01~0.015 g / L and sulfur 0.012 ~0.02 g / L. The above-mentioned nutrient solution can comprehensively provide the macroelements and trace elements required for the growth of P. chinensis, the asexual reproduction speed is fast, the probability of gene mutation is low, and a large number of plants with the same gene can be obtained, and the sample size is sufficient.

[0018] The plant clones were cultured indoors in a light incubator for 50-70 days, the culture conditions were 22.5-23.5°C, the humidity was 73-83%, the light intensity was 1800-22...

Embodiment 2

[0023] The method for screening the molecular markers related to the growth of the leaves of P. fruticosa includes clone culture, genomic DNA extraction, design of microsatellite primers, PCR amplification, and data statistics and analysis.

[0024] The composition and concentration of the nutrient solution for plant clone cultivation are: nitrogen 0.32 g / L, phosphoric anhydride 0.12 g / L, potassium oxide 0.19 g / L, magnesium oxide 0.012 g / L and sulfur 0.016 g / L. The above-mentioned nutrient solution can comprehensively provide the macroelements and trace elements required for the growth of P. chinensis, the asexual reproduction speed is fast, the probability of gene mutation is low, and a large number of plants with the same gene can be obtained, and the sample size is sufficient.

[0025] The plant clones were cultured indoors for 60 days in a light incubator, the culture conditions were 23°C, humidity 78%, light intensity 2000lux, and light-dark time 16:8h. Under the above-me...

Embodiment 3

[0030] The method for screening the molecular markers related to the growth of the leaves of P. fruticosa includes clone culture, genomic DNA extraction, design of microsatellite primers, PCR amplification, and data statistics and analysis.

[0031] The composition and concentration of the nutrient solution for plant clone cultivation are: nitrogen 0.32g / L, phosphoric anhydride 0.12g / L, potassium oxide 0.2g / L, magnesium oxide 0.013g / L and sulfur 0.017g / L. The above-mentioned nutrient solution can comprehensively provide the macroelements and trace elements required for the growth of P. chinensis, the asexual reproduction speed is fast, the probability of gene mutation is low, and a large number of plants with the same gene can be obtained, and the sample size is sufficient.

[0032] For each plant (leaves connected) in different culture lines, the number of leaves was counted, and the leaf length (the longest length of the leaf), the width of the leaf (the shortest length of th...

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Abstract

The invention discloses a method for screening molecular markers relevant to few-root duckweed leaf growth. The method comprises the steps of clone body culture, genome DNA (deoxyribonucleic acid) extraction, microsatellite primer design, PCR (polymerase chain reaction) amplification and data statistics and analysis. The method has the beneficial effects that the DNA sequences of a great amount of few-root duckweed marked by the microsatellite primers can be fast obtained; the microsatellite mark detection speed is high; the polymorphic map of the few-root duckweed Sp10, Sp14, Sp42, Sp47, Sp51, Sp52 and Sp53 genetic marker loca showing the high-degree genetic variation can be fast obtained. The leaf length and the leaf width of the few-root duckweed are obviously relevant to Sp10, Sp14, Sp42, Sp47, Sp51, Sp52 and Sp53 (P of the Sp51 site is lower than 0.05, and P of other sites is smaller than 0.001). The influence of the genotype differences on the duckweed growth and pollutant purification capability can be known; the wastewater biological treatment efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of DNA molecular markers, in particular to a method for screening molecular markers related to the growth of leaves of P. Background technique [0002] Rich, highly heterozygous and good stability, co-dominant inheritance following Mendelian segregation law, easy PCR amplification, etc., have become widely used DNA markers in recent years, and are often used in family pedigree authentication and gene linkage analysis of biological resources , genetic map construction, germplasm identification, population genetic diversity and many other research fields. [0003] In the natural environment, P. spp. basically reproduces asexually. When the environmental conditions are suitable, one generation can be cloned in 2 days. The genetic diversity of the population is low. [0004] The prior art provides a variety of detection methods for satellite markers, for example, Chinese Invention Authorized Patent Document A R...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 徐娜娜徐嘉俊朱旭辉
Owner ZHEJIANG OCEAN UNIV
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