Short peptide, application thereof and antibacterial composition obtained from short peptide

An antibacterial composition and short peptide technology, applied in the field of antibacterial compositions, can solve the problems of nutrient loss of raw materials, required equipment, material nutrient loss, etc., and achieve a good inhibitory effect.

Active Publication Date: 2017-08-08
山东泰山生力源集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, each method has limitations, either because the nutrition of the raw material is lost, or because expensive equipment is required, etc.
For example, the structure of aflatoxin is very stable and can be decomposed at 270°C. If heat treatment is used, the higher the temperature and the longer the heating time, the higher the removal rate of aflatoxin. However, it is obvious that the heating method will easily cause the nutrient loss of the material, and Equipment requiring high voltage resistance

Method used

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  • Short peptide, application thereof and antibacterial composition obtained from short peptide
  • Short peptide, application thereof and antibacterial composition obtained from short peptide
  • Short peptide, application thereof and antibacterial composition obtained from short peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The embodiment of the present invention provides a kind of short peptide, this short peptide is first isolated from Bacillus megaterium fermentation liquid, specifically as follows:

[0027] Microorganisms used:

[0028] Aspergillus flavus A.flavus NRRL 3357: cultivated in PDA medium (200g potato extract, 20g glucose, 20g agar, 1L water).

[0029] Bacillus megaterium (CGMCC7086, preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee): cultured in beef extract peptone medium (beef extract 3g, peptone 10g, sodium chloride 5g, 1L water).

[0030] Purification and Identification:

[0031] 12 liters of Bacillus megaterium were cultured (160 rpm, 37°C, 3 days), and the fermentation broth was centrifuged at 8,000 r / min for 20 minutes. The centrifuged supernatant was precipitated with 80% ammonium sulfate, and after the precipitate was freeze-dried, it was chromatographed on Sephadex G-25 with a flow rate of 5 mL / min and...

Embodiment 2

[0050] Antibacterial test on solid medium

[0051] The 24 kinds of short peptides (all L-type) obtained above were subjected to the antibacterial effect test on Aspergillus flavus, respectively named as: a, b, c, d, e, f, g, h, i, j, k ,l,m,n,o,p,q,r,s,t,u,v,w,x, and compared with laboratory disinfectant-75% alcohol and strong fungicide-1% potassium permanganate Carry out the evaluation of antibacterial effect (repeat test 3 times). Among them, the specific test methods are:

[0052] Prepare solid PDA medium, pour the PDA medium into a 96-well plate to solidify; spread 4 microliters on the PDA medium for 10 6 Aspergillus flavus spores / ml, then take 10 microliters of various short peptides of 16 mg / ml, 75% alcohol, and 1% potassium permanganate and apply them to each sample well on a 96-well plate, and cultivate them at 28°C for 3 days. Days to observe the antibacterial results.

[0053] Such as Figure 4 As shown, the obtained 24 kinds of short peptides have a certain inh...

Embodiment 3

[0055] Bacteriostasis test in crop storage

[0056] The crops in this embodiment can be peanuts, corn, rice, cottonseed, nuts, etc., wherein, taking peanut storage as an example, the details are as follows:

[0057] First of all, it should be noted that since the 24 short peptides have been proved to have good antibacterial effects by Example 2, this example only takes Asp-Orn, Asp-Asn and Asp-Asn and Asp-Asn as examples for testing, and at the same time , in order to judge the impact of the configuration of each peptide on the antibacterial effect, the synthesis of different configurations was carried out by taking the above three peptides as examples. The resulting short peptides of different configurations were made into solutions of different concentrations, sprayed onto peanuts, and cultivated at 28°C for 7 days to observe the growth of Aspergillus flavus (as a control comparison: each peanut was inoculated with 10 5 aflatoxin spores).

[0058] Depend on Figure 5 It c...

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Abstract

The invention provides a short peptide, application thereof and an antibacterial composition obtained from the short peptide, and belongs to the technical field of biology. The short peptide can be effectively applied to the inhibition of growth of Aspergillus flavus and the biosynthesis of aflatoxin. The short peptide has the following general formula structure: (shown in the description), wherein X represents asparagines or ornithine, when n is equal to 0, m is a positive integer between 2 to 9; when n is equal to 1, m is a positive integer between 1 to 8. The short peptide can be applied to the inhibition of the growth of the Aspergillus flavus.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a short peptide, its application and an antibacterial composition obtained therefrom. Background technique [0002] Aflatoxins are mainly produced by Aspergillus flavus and Aspergillus parasiticus, which are highly toxic, teratogenic and carcinogenic. Crops such as peanuts, corn, rice, cottonseed, and nuts are easily contaminated by aflatoxins. Aflatoxin B1 is the most toxic natural carcinogen known, which mainly causes liver cancer. [0003] Physical, chemical and biological methods are commonly used to remove aflatoxin from raw materials. Physical methods such as heating, adsorption, extraction, gamma ray irradiation, ultraviolet irradiation, etc. Chemical methods such as treatment with ozone, ammonia, chlorine, hydrogen peroxide, and formaldehyde. Biological methods, such as detoxification of raw materials by microbial fermentation, and inhibition of toxin productio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K5/072C07K5/09C07K5/11C07K7/06A01N37/46A01P3/00
CPCC07K14/32A23L3/3526A61P31/04A23V2002/00A61K38/00A23V2250/55
Inventor 孔青陈玉洁姚瑶许舒静谢翔
Owner 山东泰山生力源集团股份有限公司
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