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Modifier for microbial adhesion rate and application thereof

A technology of microorganisms and adhesion rate, which is applied in the direction of anti-inflammatory agents, antifungal agents, antiviral agents, etc., can solve the problem of changing the adhesion rate of pathogenic bacteria, so as to improve the probability and speed, increase the adhesion rate, prevent and treat a large number of effect of disease

Inactive Publication Date: 2017-08-11
CHENGDU JIYANGWU TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These technical problems are also due to the fact that the adhesion rate of pathogenic bacteria has not been (selectively) changed.

Method used

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  • Modifier for microbial adhesion rate and application thereof

Examples

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Effect test

Embodiment 1

[0104] This embodiment provides a microorganism adhesion rate regulator, which contains a surfactant, and the surfactant is lichenysin. The microorganism adhesion rate regulator is a liquid type, and its surface tension is 55mN / m.

[0105] 1.1 The microbial adhesion rate regulator is prepared with reference to the following method:

[0106] 1.1.1 Lactobacillus salivarius strain (Lactobacillus salivarius, ATCC 11741) was selected for cultivation. Culture conditions: 28°C, 5% inoculum, MRS as the medium, anaerobic culture, the initial pH of the MRS medium is 5.5, and culture for 15 hours.

[0107] 1.1.2 After the cultivation, centrifuge at 4500rpm for 10min to collect Lactobacillus salivarius cells.

[0108] 1.1.3 Bacillus licheniformis (Bacillus licheniformis, ATCC 39307) was selected for cultivation to obtain a culture solution containing lipopeptides, especially lichenin (a natural surfactant from microorganisms). Medium formula: soluble starch 20.0g / L, NH 4 NO 3 4.0g / L...

Embodiment 2

[0144] This embodiment provides a microbial adhesion rate regulator, which contains a surfactant, NaCl, and lactic acid. Surfactant is surfactin (surfactin), content is 14.5mg / L, lower than its critical micelle concentration (CMC) 69mg / L, the surface tension of this microbial adhesion rate modifier is 55mN / m, pH value is 5.5 .

[0145] 2.1 The microbial adhesion rate regulator and related probiotic preparations are prepared by referring to the following method:

[0146] 2.1.1 Lactobacillus casei (Lactobacillus casei, ATCC 393) was selected. Inoculate the fresh bacterial solution cultivated overnight and inoculate it into the MRS medium, the inoculum amount is 1%, the cultivation temperature is 28°C, and the anaerobic cultivation is left in the incubator for about 15 hours. After the cultivation, centrifuge at 4500rpm for 10min to collect Lactobacillus casei cells.

[0147] 2.1.2 Bacillus subtilis (Bacillus subtilis, ATCC2233), put 50ml of culture solution in a 150ml Erlenme...

Embodiment 3

[0199] This embodiment provides a microbial adhesion rate regulator, which contains a surfactant, NaCl, and lactic acid. Surfactant is surfactin, content is 16mg / L, lower than its critical micelle concentration (CMC) 69mg / L, the surface tension of this microbial adhesion rate modifier is 40mN / m, pH is 5.5, NaCl content is 9g / L.

[0200] 3.1 The preparation method of the microbial adhesion rate regulator provided in this example is basically the same as step 2.1 in Example 2.

[0201] In addition, this embodiment also provides a probiotic preparation, which contains the above-mentioned microorganism adhesion rate regulator and inactivated Lactobacillus casei. The surface tension of the probiotic preparation is 40mN / m, the pH is 5.5, the NaCl content is 9g / L, the surfactant content is 16mg / L, and the inactivated probiotic content is 1×10 8 pieces / ml.

[0202] 3.2 The preparation method of the probiotic preparation provided in this example is basically the same as step 2.1 in...

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Abstract

The invention discloses a modifier for a microbial adhesion rate and application thereof, and relates to the technical field of biology. The modifier for the microbial adhesion rate is suitable for regulating the adhesion rate of microorganisms with the mucous membranes or the skin, and contains a surfactant, and the concentration of the surfactant is lower than the critical micelle concentration (CMC) of the surfactant. The modifier for the microbial adhesion rate can increase the probability and speed of the contact of the microorganisms and the mucosal cells or skin cells. Meanwhile, by the adoption of the surfactant, the cell-surface hydrophobicity of the microorganisms is changed, the microbial adhesion rate of the beneficial microorganisms with the mucosal epithelial cells or the skin cuticle is increased, the microorganisms are easily colonized on the mucous membranes or the skin, the microorganisms can continuously take effect for a long time, the modifier has broad application prospects.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a microorganism adhesion rate regulator and its application. Background technique [0002] Many microorganisms in nature can live on and near the surface of mucous membranes and skin of animals, directly affecting the health of the host. Bacteria and fungi in these microorganisms are generally divided into pathogenic bacteria, opportunistic pathogenic bacteria, and probiotics according to the quality and degree of impact on the health of the host. In this sense, probiotics are bacteria and fungi that are beneficial to the health of the host. [0003] Through adhesion and colonization, exogenously administered probiotics can produce a variety of beneficial effects on the host: including adjusting the immune response, preventing and treating allergies, anti-cancer, inhibiting harmful bacteria, lowering blood pressure, lowering cholesterol, lowering blood lipids, regulating the nervous...

Claims

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Application Information

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IPC IPC(8): A61K35/747A61K35/744A61K35/745A61K35/742A61K47/12A61K47/02A61K47/42A61K47/18A61K47/26A61K47/46A61P15/02A61P1/02A61P27/02A61P17/00A61P11/02A61P11/06A61P37/08A61P25/00A61P35/00A61P11/00A61P37/02A61P31/04A61P1/00A61P31/10A61P29/00A61P1/04A61P31/14A61P31/02A61P11/14G01N11/00
CPCA61K9/0014A61K9/0034A61K9/006A61K35/742A61K35/744A61K35/745A61K35/747A61K47/02A61K47/12A61K47/18A61K47/183A61K47/26A61K47/42A61K47/46G01N11/00
Inventor 杜斌
Owner CHENGDU JIYANGWU TECH CO LTD
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