Bacillus subtilis hmb28948 and its application

A Bacillus subtilis and bacterial strain technology, applied in the field of biological control, can solve the problems of affecting the normal growth of cotton, poor chemical control effect, and the death of cotton seedlings in pieces, and achieves strong drug effect persistence, broad antibacterial spectrum and low cost. Effect

Active Publication Date: 2020-02-14
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cotton blight seriously affects the normal growth of cotton, and often leads to the death of cotton seedlings after the onset, which is an urgent problem to be solved
[0003] The control of cotton blight is mainly based on chemical control, including dressing, coating or soaking seeds with chemical agents before sowing, and spraying and root irrigation with chemical agents after sowing, but the effect of chemical control is poor, and there is still pollution. Environment, long-term drug resistance and high cost are prone to problems

Method used

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  • Bacillus subtilis hmb28948 and its application
  • Bacillus subtilis hmb28948 and its application
  • Bacillus subtilis hmb28948 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The preparation of embodiment 1HMB28948 microbial bacterial agent

[0051] Follow the steps below:

[0052] (1) Strain activation: the Bacillus subtilis strain HMB28948 stored at -80°C (this strain has been preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures on October 28, 2016, and the preservation number is CGMCCNo.13212) Activate (30°C) on LB plate medium (its composition and weight ratio are: tryptone 10g, yeast extract 5g, sodium chloride 5g, agar powder 15g, water 1000mL), pick a single colony on LB Culture on slant medium (its composition and weight ratio is: tryptone 10g, yeast extract 5g, sodium chloride 5g, agar powder 15g, water 1000mL) at 30°C for 12 hours to obtain an activated strain;

[0053] (2) Preparation of seed liquid: make LB liquid medium according to conventional methods (its composition and weight ratio are: tryptone 10g, yeast extract 5g, sodium chloride 5g, water 1000mL), and put it in a...

Embodiment 2

[0056] Embodiment 2 Antagonism test of HMB28948 bacterial strain of the present invention to R. solani of cotton

[0057] (1) Source of R. solanacearum tested: R. solanacearum RH-2 strain was collected from a cotton blight disease strain in Qiu County, Handan City, Hebei Province. It was isolated and purified by the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences. The Plant Pathology Department of the Conservation College identified it as Rhizoctonia solani (Rhizoctonia solani), and the pathogenicity test showed strong pathogenicity.

[0058] (2) Test method

[0059] Plate determination test: first activate and cultivate R. solani RH-2 on a PDA plate for 3 days, then use a puncher with a diameter of 6mm to punch holes in the edge area of ​​the colony to make a bacterial sheet, and then place the bacterial sheet of R. solani Transfer to another PDA plate center, then the bacillus subtilis HMB28948 activated in the step (1) of embodiment 1 is s...

Embodiment 3

[0064] Embodiment 3 HMB28948 bacterial strain of the present invention is to the comparative test of control effect of cotton blight

[0065] (1) Test treatment:

[0066] (1) HMB28948 microbial agent: the HMB28948 liquid preparation prepared in Example 1 was diluted 50 times with water.

[0067] (2) Blank control: clear water

[0068] (2) Test method:

[0069] First, mix the vermiculite and cotton field soil according to the ratio of 1:1, sterilize under high pressure at 121 ℃ for 1 hour, and then sterilize again under the same conditions the next day. Inoculate the suspension of Rhizoctonia solani mycelium in 500 grams of soil (about 10 7 per milliliter) 10 milliliters, stir evenly, fill in a plastic flower pot with a diameter of 20 cm, and compact it. The cotton variety was Jifeng 106, and the seeds were soaked in the 50-fold water dilution of the HMB28948 liquid preparation prepared in Example 1 for half an hour before sowing; the seeds were soaked in clear water for ha...

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Abstract

The invention discloses a Bacillus subtilis strain HMB28948, which is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the preservation number is CGMCC No.13212. The invention also discloses the microbial bacterial agent produced by the strain. The Bacillus subtilis HMB28948 of the present invention has a high drug effect on cotton blight, with an average control effect of more than 79.0%; secondly, using the Bacillus subtilis HMB28948 of the present invention to prevent and control cotton blight is not easy to produce drug resistance, and the drug effect is durable; in addition , the Bacillus subtilis HMB28948 of the present invention has a wide antibacterial spectrum, and has good inhibitory effects on cotton Verticillium dahliae, cotton Fusarium wilt, cucumber target spot fungus and tomato Botrytis cinerea except cotton blight.

Description

technical field [0001] The invention belongs to the field of biological control, and in particular relates to a bacillus subtilis HMB28948, a microbial agent produced by using the bacillus subtilis, and also relates to their use in preventing and treating plant diseases such as cotton blight. Background technique [0002] Cotton blight is a cotton seedling disease caused by Rhizoctonia solani Kühn. Its main symptoms are: cotton seeds are infected before germination and cause rotten seeds, and cotton seeds are germinated before they emerge. Infection causes rotten buds; after the cotton seedlings are unearthed, they are damaged, and at the initial stage, yellow-brown lesions are formed near the base of the soil surface, and the lesions gradually expand and surround the entire base, showing obvious constriction, and the diseased seedlings wilt, fall and die; when the diseased seedlings are pulled up, the stem base The lower cortex remains in the soil, and only the sharp rattai...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/125
CPCA01N63/00C12N1/20C12N1/205C12R2001/125
Inventor 郭庆港马平李社增鹿秀云张晓云王莹王培培赵卫松张丽红
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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