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Preparation method of composite vegetable protein fertilizer

A plant protein and fertilizer technology, which is applied in the preparation of organic fertilizers, fertilizers made from biological waste, and the molding or processing of animal feeds, can solve the problems of restricting the comprehensive utilization of flaxseed meal, reducing the nutritional value of protein, etc. The effect of the body's immunity

Inactive Publication Date: 2017-08-29
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, these two dregs are basically used as low-cost animal feed, and have not been fully and effectively utilized. One of the main reasons is that sunflower meal contains more phenolic compounds such as chlorogenic acid. When extracting sunflower seed protein, chlorogenic acid is easily oxidized into green quinone, which makes the protein product dark brown or brown. Once quinone is formed, it is easy to combine with polar groups and amino acids of protein molecules, and it is not easy to be absorbed by non-ruminant animals. Digestion with poultry, etc., reduces the nutritional value of protein, and chlorogenic acid is also an inhibitor of arginine, so chlorogenic acid is considered an anti-nutritional factor; cyanogenic glycoside-based toxic substances in flaxseed meal, It greatly limits the comprehensive utilization of flaxseed meal, and cannot maximize the advantages of flaxseed resources into economic advantages

Method used

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  • Preparation method of composite vegetable protein fertilizer
  • Preparation method of composite vegetable protein fertilizer

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Effect test

Embodiment 1

[0031] A preparation method of compound plant protein fertilizer, the concrete steps are as follows:

[0032] (1) Preparation of solid-state fermentation medium: after mixing sunflower seed meal, linseed meal and bran at a mass ratio of 3:5:2, add water to obtain a solid-state fermentation medium with a water content of 40%; the sunflower seeds Meal and linseed meal are dehulled sunflower meal and linseed meal;

[0033] (2) Preparation of strain seed solution:

[0034] A. Preparation of Lactobacillus plantarum seed solution: activate the thallus in the bacterial preservation tube for 3 generations, then inoculate it into MRS medium, culture at 37°C for 18 hours, and let it stand for cultivation to obtain the bacterial seed solution;

[0035] B. Preparation of Lactobacillus licheniformis seed liquid: activate the thalline in the bacteria preservation tube for 3 generations, then inoculate it into a nutrient agar medium, cultivate it at 26°C for 24 hours, and cultivate it stati...

Embodiment 2

[0045] A preparation method of compound plant protein fertilizer, the concrete steps are as follows:

[0046] (1) Preparation of solid-state fermentation medium: after mixing sunflower seed meal, linseed meal and bran at a mass ratio of 2:2:1, add water to obtain a solid-state fermentation medium with a water content of 50%; the sunflower seeds Meal and linseed meal are dehulled sunflower meal and linseed meal;

[0047] (2) Preparation of strain seed solution:

[0048]A. Preparation of Lactobacillus plantarum seed liquid: activate the thallus in the bacteria preservation tube for 3 generations, then inoculate it into MRS medium, cultivate it at 35°C for 24 hours, and cultivate it statically to obtain the bacterial seed liquid;

[0049] B. Preparation of Lactobacillus licheniformis seed liquid: activate the thalline in the bacteria preservation tube for 3 generations, then inoculate it into a nutrient agar medium, cultivate it at 30° C. for 18 hours, and cultivate it staticall...

Embodiment 3

[0059] A preparation method of compound plant protein fertilizer, the concrete steps are as follows:

[0060] (1) Preparation of solid-state fermentation medium: after mixing sunflower seed meal, linseed meal and bran at a mass ratio of 5:3:2, add water to obtain a solid-state fermentation medium with a water content of 60%; the sunflower seeds Meal and linseed meal are dehulled sunflower meal and linseed meal;

[0061] (2) Preparation of strain seed solution:

[0062] A. Preparation of Lactobacillus plantarum seed solution: activate the thallus in the bacteria preservation tube for 3 generations, then inoculate it into MRS medium, cultivate it at 36°C for 20 hours, and cultivate it statically to obtain the bacterial seed solution;

[0063] B. Preparation of Lactobacillus licheniformis seed liquid: activate the thalline in the bacteria preservation tube for 3 generations, then inoculate it into a nutrient agar medium, cultivate it at 28°C for 20 hours, and cultivate it static...

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Abstract

The invention discloses a preparation method of a composite vegetable protein fertilizer. The method comprises the following specific steps of (1) mixing sunflower seed meal, flaxseed meal and bran at the ratio and then adding water to a solid fermentation medium; (2) preparing a strain seed solution; (3) preparing a mixed strain; (4) inoculating the mixed strain into the solid fermentation medium and carrying out mixed fermentation; and (5) drying and crushing the fermented product to obtain the composite vegetable protein fertilizer. According to the method, anti-nutritional factors and toxic substances can be eliminated in the fermentation process, the content of protein is improved, the fertilizer is high in crude protein content and relatively multiple microelements and bioactive substances can be provided for animals and plants.

Description

technical field [0001] The invention relates to the technical field of biological material processing, in particular to a method for preparing compound vegetable protein fertilizer by fermenting mixed strains. Background technique [0002] Both sunflower seeds and linseeds are one of the main oil crops in my country. The output of cakes obtained after oil extraction is very large, and their protein content is as high as 30-40%. The amino acids are complete, and they are plant protein sources with high nutritional value. , Moreover, the content of B family, niacin, thiamine and choline in sunflower seed meal is high, the content of trace elements calcium, potassium and methionine are also higher than those of general cakes, and the protein efficiency ratio of sunflower seed is 2:1. The yield (TD) is 82%, the biological value (BV) is 70%, and the net utilization rate (NPU) is 57%. The linseed meal also contains lignans, a-linolenic acid and other biologically active substances, ...

Claims

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Application Information

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IPC IPC(8): A23K10/37A23K10/12A23K40/00C05G3/00C05F17/00
CPCA23K10/12A23K10/37A23K40/00C05F5/002C05F17/80C05G3/00C05F11/08Y02P60/87Y02W30/40
Inventor 于秋生尹义敏郝祥蕊陈正行陈天祥张红艳陈林李珍妮冯伟
Owner JIANGNAN UNIV
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