Trichoderma viride and culture method thereof
A technology of green Trichoderma and its cultivation method, which is applied in the field of bioengineering, can solve the problems of high cost of Trichoderma, limit the wide use of Trichoderma, and harsh production conditions, so as to increase the utilization rate of equipment, have good environmental benefits, and benefit the industry effect
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Embodiment 1
[0037] A kind of culture method of Trichoderma viride, its concrete steps are:
[0038] (1) Activation of Trichoderma viride strain
[0039] Place the Trichoderma viride strain on a PDA medium plate, culture it at 26°C for 4 days, and activate it;
[0040] (2) Preparation of liquid strains;
[0041] The activated Trichoderma viride strain was transferred to PD medium, cultured at 180rpm, 26°C for 48h;
[0042] Then transfer to Trichoderma viride liquid culture medium, 180rpm, 28 ℃ culture 48h;
[0043] Described Trichoderma viride liquid culture medium is by weight (w / w): glucose 0.3%, corn flour 2%, yeast extract powder 0.5%, magnesium sulfate 0.05%, potassium dihydrogen phosphate 0.04%, add water to 1000mL; The Erlenmeyer flask containing the above medium was sterilized in an autoclave at 121°C for 30 minutes;
[0044] The specific transfer process is as follows: on the aseptic operating table, use 10mL sterile water to make the bacteria suspension in the test tube slant...
Embodiment 2
[0053] A kind of culture method of Trichoderma viride, its concrete steps are:
[0054] (1) Activation of Trichoderma viride strain
[0055] Place the Trichoderma viride strain on a PDA medium plate, culture it at 30°C for 4 days, and activate it;
[0056] (2) Preparation of liquid strains
[0057] The activated Trichoderma viride strain was transferred to PD medium, cultured at 180rpm, 28°C for 24h;
[0058]Then transfer to Trichoderma viride liquid culture medium, 180rpm, 28 ℃ culture 48h;
[0059] Described Trichoderma viride liquid culture medium is by weight (w / w): glucose 0.5%, corn flour 1%, yeast extract powder 0.5%, magnesium sulfate 0.05%, potassium dihydrogen phosphate 0.04%, add water to 1000mL; The Erlenmeyer flask containing the above medium was sterilized in an autoclave at 121°C for 30 minutes;
[0060] The specific transfer process is as follows: on the aseptic operating table, use 10mL sterile water to make the bacteria suspension in the test tube slant c...
Embodiment 3
[0069] A kind of culture method of Trichoderma viride, its concrete steps are:
[0070] (1) Activation of Trichoderma viride strain
[0071] Place the Trichoderma viride strain on a PDA medium plate, culture it at 26°C for 7 days, and activate it;
[0072] (2) Preparation of liquid strains;
[0073] The activated Trichoderma viride strain was transferred to PD medium, cultured at 180rpm, 27°C for 36h;
[0074] Then transfer to Trichoderma viride liquid culture medium, 180rpm, 28 ℃ culture 48h;
[0075] Described Trichoderma viride liquid culture medium is by weight (w / w): glucose 0.3%, corn flour 2%, yeast extract powder 0.5%, magnesium sulfate 0.05%, potassium dihydrogen phosphate 0.04%, add water to 1000mL; The Erlenmeyer flask containing the above medium was sterilized in an autoclave at 121°C for 30 minutes;
[0076] The specific transfer process is as follows: on the aseptic operating table, use 10mL sterile water to make the bacteria suspension in the test tube slant...
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