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A compound bacteria enzyme preparation for improving water quality and its preparation method and application

A technology for compound bacterial enzymes and preparations, which is applied in the field of compound bacterial enzyme preparations, the preparation of the above-mentioned composite bacterial enzyme preparations, and water quality improvers, and can solve problems such as uneven temperature control, loss of bacterial enzyme activity, and large loss of bacterial enzyme activity.

Active Publication Date: 2021-07-06
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, aquaculture water quality improver microorganisms or enzyme preparation granules generally rely on two methods: disc granulation and extrusion granulation: the disc granulation drying particle size is larger, the drying process is simple, and the temperature control is uneven and relatively difficult. High, the loss of the enzyme activity of the strain is relatively large, and the general loss rate reaches about 50%. It can reach above 70 degrees, and it also caused a great loss to the enzyme activity of the bacteria

Method used

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  • A compound bacteria enzyme preparation for improving water quality and its preparation method and application
  • A compound bacteria enzyme preparation for improving water quality and its preparation method and application
  • A compound bacteria enzyme preparation for improving water quality and its preparation method and application

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Embodiment 1

[0033] A compound bacterial enzyme preparation for improving water quality is prepared from the following components: bacillus subtilis, alkaline protease, neutral protease, amylase, glucoamylase, glucose oxidase and carrier. Among them, each gram of the obtained compound bacteria enzyme preparation contains Bacillus subtilis 3 × 10 9 1, alkaline protease 10000U, neutral protease 1000U, amylase 200U, glucoamylase 2000U, grape oxidase 50U, carrier 400mg. That is, in the obtained compound bacteria enzyme preparation, the content of Bacillus subtilis is 3×10 9 per g, the content of alkaline protease is 10000U / g, the content of neutral protease is 1000U / g, the content of amylase is 200U / g, the content of glucoamylase is 2000U / g, the content of grape oxidase is 50U / g, and the content of carrier is 400mg / g.

[0034] The carrier is formed by mixing starch, Jiayi powder, bentonite, nanoscale talcum powder and xanthan gum according to the weight ratio of 3:1:2:2:1.

[0035] The gran...

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Abstract

The invention relates to a compound bacteria enzyme preparation for improving water quality, and also relates to a preparation method and application of the compound bacteria enzyme preparation. The complex bacteria enzyme preparation includes the following components: Bacillus subtilis, alkaline protease, neutral protease, amylase, glucoamylase, glucose oxidase and carrier, wherein Bacillus subtilis belongs to a kind of liquid fermentation and solid fermentation production. Alkaline protease and neutral protease belong to the fermentation production of Bacillus subtilis and yeast genetically engineered bacteria; amylase belongs to α-amylase, β-amylase and γ-amylase; A kind produced by the fermentation of engineering bacteria, and a kind of glucose oxidase produced by the fermentation of Aspergillus niger and Penicillium. The compound bacterial enzyme preparation of the invention can assist in decomposing organic matter such as residual bait and feces in aquaculture ponds, improve pond water quality and bottom quality, reduce ammonia nitrogen and nitrite in aquaculture water, and improve water transparency.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and relates to a water quality improving agent, in particular to a compound bacterial enzyme preparation for improving water quality, and also relates to a preparation method and application of the compound bacterial enzyme preparation. Background technique [0002] At present, in the process of aquaculture, water quality is the main factor that determines the production of aquaculture. The main factor causing the deterioration of water quality is that a large amount of residual bait and feces accumulate at the bottom of the pond, and water microorganisms cannot quickly utilize and convert the residual bait and feces into nutrients needed for algae growth. With the eutrophication of the water body, the algal phase of the water body has gradually changed to harmful algae such as dinoflagellates, euglena, and cyanobacteria. At the same time, algae respiration at night will rapidly reduce the di...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/14C12N1/16C12N9/04C12N9/26C12N9/34C12N9/50C02F3/34C12R1/125C02F101/38
CPCC02F2101/38C12N1/20C12N9/0006C12N9/2414C12N9/2425C12N9/2428C12N9/50C12Y101/03004C12Y302/01001C12Y302/01002C12Y302/01003
Inventor 蔡玉勇田相利董双林
Owner OCEAN UNIV OF CHINA
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