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Method for extracting fucoxanthin from brown seaweeds

A fucoxanthin and brown algae technology, applied in the biological field, can solve the problems of low extraction efficiency, poor extraction effect, loss, etc., and achieve the effects of improving dissolution rate, saving time, and increasing output

Inactive Publication Date: 2017-09-22
XIAMEN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The inventor found that fucoxanthin has the characteristics of photosensitivity and heat sensitivity, and is easily lost during the extraction process
Using common solvent extraction method, supercritical CO 2 Fucoxanthin in brown algae is extracted by extraction method, ultrasonic assisted extraction method, etc., the extraction efficiency is low, and the extraction effect is poor

Method used

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  • Method for extracting fucoxanthin from brown seaweeds
  • Method for extracting fucoxanthin from brown seaweeds
  • Method for extracting fucoxanthin from brown seaweeds

Examples

Experimental program
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Embodiment 1

[0046] A method for extracting fucoxanthin from brown algae provided in this embodiment comprises:

[0047] Pretreatment: Wash the brown algae to remove sediment, freeze-dry and pulverize, the particle size is 0.09-0.125mm.

[0048] Compound enzyme preparation: pectinase: cellulase=1:3.

[0049] Extraction: Mix the pretreated brown algae powder and compound enzyme, add ethanol and stir, then send it into the microwave chamber, low-temperature microwave co-extraction, the microwave temperature is 45°C, and the microwave time is 4min (extraction times are 3 times, respectively extracting 1min, 2min, 1min).

[0050] Separation: After microwave, solid-liquid separation is carried out, and the liquid phase is filtered through a 0.45um membrane to remove impurities, and then freeze-dried to obtain fucoxanthin.

Embodiment 2

[0052] A method for extracting fucoxanthin from brown algae provided in this embodiment comprises:

[0053] Pretreatment: Wash the brown algae to remove sediment, freeze-dry and pulverize, the particle size is 0.15-0.25mm.

[0054] Compound enzyme preparation: pectinase: cellulase=2:1.

[0055] Extraction: Mix the pretreated brown algae powder and compound enzyme, add ethanol and stir, then send it to the microwave chamber, low-temperature microwave co-extraction, the microwave temperature is 40°C, and the microwave time is 8 minutes (the number of extractions is 4 times, each time 2 minutes) . Among them, the mass ratio of brown algae powder and compound enzyme is 20:3, and the solid-liquid ratio of brown algae powder and ethanol is 1:30g / mL.

[0056] Separation: After microwave, solid-liquid separation is carried out, and the liquid phase is filtered through a 0.45um membrane to remove impurities, and then freeze-dried to obtain fucoxanthin.

Embodiment 3

[0058] A method for extracting fucoxanthin from brown algae provided in this embodiment comprises:

[0059] Pretreatment: Wash the brown algae to remove sediment, freeze-dry and pulverize, the particle size is 0.15-0.25mm.

[0060] Compound enzyme preparation: pectinase: cellulase = 1:1.

[0061] Extraction: Mix the pretreated brown algae powder and compound enzyme, add ethanol and stir, and then send it to the microwave room for low-temperature microwave co-extraction, the microwave temperature is 55°C, and the microwave time is 6min (extraction times are 4 times, each 1.5min ). Wherein, the mass ratio of brown algae powder and compound enzyme is 20:1, and the solid-liquid ratio of brown algae powder and ethanol is 1:20g / mL.

[0062] Separation: After microwave, solid-liquid separation is carried out, and the liquid phase is filtered through a 0.45um membrane to remove impurities, and then freeze-dried to obtain fucoxanthin.

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Abstract

The invention provides a method for extracting fucoxanthin from brown seaweeds, and relates to the technical field of biology. The method for extracting the fucoxanthin from the brown seaweeds comprises the following steps: after freeze-drying the brown seaweeds, crushing to obtain brown seaweed powder; after mixing the brown seaweed powder and a complex enzyme, adding ethanol to obtain a raw material solution; carrying out microwave extraction on the raw material solution for 3min to 8min to obtain an extracting solution, wherein the complex enzyme is prepared from pectinase and cellulase at the mass ratio of 1 : (0.5 to 4); carrying out solid-liquid separation on the extracting solution. The microwave extraction is cooperated with enzymolysis, and cell walls of the brown seaweeds can be rapidly broken, so that effective components in cells are dissolved out; the method has moderate reaction conditions, is simple to operate and is safe and environmentally friendly. Two extracting methods are combined, and the extracting quality is high; the fucoxanthin in the brown seaweeds can be effectively protected and is not damaged. The yield of an obtained extract is great, and the method has the advantages of time saving, reduction of the usage amount of a solvent, low energy consumption and the like.

Description

technical field [0001] The invention relates to the field of biotechnology, and in particular to a method for extracting fucoxanthin from brown algae. Background technique [0002] Fucoxanthin, also known as fucoxanthin and fucoxanthin, is an important fat-soluble carotenoid widely distributed in seaweed, marine phytoplankton, aquatic shells and other marine animals and plants. Among them, brown algae has the highest content, which is an important functional active substance in edible brown algae such as kelp, wakame, and hijiki, and is also a substance that makes brown algae appear brown. Its structure contains multiple conjugated double bonds, can be dissolved in organic solvents such as ethanol, acetone, methanol, etc., and has special structures such as propadiene and alkylene oxide, and has poor stability and is easy to oxidize. Fucoxanthin has extremely high medicinal value and has a special effect on anti-tumor, which can block tumor cell mitosis and induce cell apop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D303/32C07D301/32
CPCC07D303/32C07D301/32
Inventor 徐苏廖文超严滨许美兰程蔚陈静萍
Owner XIAMEN UNIV OF TECH
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