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Saliva diagnostic sensor and preparation method and application thereof

A sensor and saliva technology, which is applied in the field of saliva diagnosis and clinical detection, can solve problems such as large environmental interference, high cost, and difficult to eliminate, and achieve the effect of simple preparation process, simple operation, and good accuracy

Inactive Publication Date: 2017-09-22
苏州纳葛诺斯生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These two methods have high analysis and detection sensitivity and accuracy, but the sample processing is time-consuming and laborious, the environment is disturbed, it is difficult to eliminate, and the reproducibility is not good. The requirements for the mass spectrometer detector are very high, which will interfere with the analysis results.
In addition, due to expensive and cumbersome chromatographic equipment, long detection cycle and high cost, the detection method is difficult to standardize, which limits its application in clinical diagnosis

Method used

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  • Saliva diagnostic sensor and preparation method and application thereof
  • Saliva diagnostic sensor and preparation method and application thereof
  • Saliva diagnostic sensor and preparation method and application thereof

Examples

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preparation example Construction

[0046] Another aspect of the embodiments of the present invention provides a method for preparing a saliva diagnostic sensor comprising:

[0047]The liquid-phase reaction system containing graphene oxide, metal ions and ethanol is ultrasonically reacted to prepare a composite nano-roll material. The composite nano-roll material refers to the curling of the graphene material with metal nanoparticles grown in situ on the surface and wrapping metal nanoparticles to form a nanovolume structure;

[0048] The saliva diagnostic sensor is prepared by coating the dispersion liquid of the composite nano-volume material on the metal surface and drying it.

[0049] Further, the metal ions include gold ions, chloroauric acid ions, silver ions, copper ions, platinum ions or chloroplatinic acid ions, etc., but are not limited thereto.

[0050] In some embodiments, the concentration of metal ions in the liquid phase reaction system is preferably 0.1 mol / L-1.5 mol / L.

[0051] In some embodim...

Embodiment 1

[0075] Example 1 see figure 1 A schematic diagram of the construction and application of a saliva diagnostic sensor in this embodiment is shown, which will be described in detail as follows.

[0076] 1. A preparation method of a saliva diagnostic sensor comprising:

[0077] 1) A total of 15 mL of a mixture containing graphene oxide, chloroaurate ions, and ethanol was prepared in an ultrasonic environment to obtain a composite nanoscroll material of graphene curl-wrapped metal nanoparticles. In the aforementioned mixed solution, the concentration of graphene oxide was 0.5 mg / mL, the concentration of chloroauric acid ions was 0.5 mmol / L, and the volume ratio of water and ethanol was 8:7. The ultrasonic power is 300W, and the ultrasonic response time is 90min.

[0078] 2) The dispersion of the prepared composite nanoscroll material was drop-coated on the cleaned and sputtered glass with a gold film with a thickness of about 300 nm, and dried to obtain the saliva diagnostic sens...

Embodiment 2

[0090] 1. A preparation method of a saliva diagnostic sensor comprising:

[0091] 1) A total of 15 mL of a mixture containing graphene oxide, chloroaurate ions, and ethanol was prepared in an ultrasonic environment to obtain a composite nanoscroll material of graphene curl-wrapped metal nanoparticles. In the aforementioned mixed solution, the concentration of graphene oxide is 0.2 mg / mL, the concentration of chloroplatinic acid ions is 0.8 mmol / L, and the volume ratio of water and ethanol is 10:5. The ultrasonic power is 200W, and the ultrasonic response time is 60min.

[0092] 2) The dispersion of the prepared composite nanoscroll material is drop-coated on a silicon wafer that has been cleaned and sputtered with a gold film with a thickness of about 200 nm, and dried to obtain the saliva diagnostic sensor. The yield is about 75%.

[0093] 2. Gastric cancer diagnosis using the saliva diagnostic sensor

[0094] Drop 4 μL of saliva sample on the surface of the saliva diagnos...

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Abstract

The invention discloses a saliva diagnostic sensor. The sensor comprises a metal surface and a composite nano coiled material distributed on the metal surface; the composite nano coiled material refers to metal nano particles generated when the metal surface is wrapped with a coiled graphene material. The invention further discloses a preparation method and application of the saliva diagnostic sensor. The saliva diagnostic sensor excellently strengthens the Raman scattering effect and maintains good stability in saliva, the detection process for disease markers in the saliva is simple, and the sensor is low in cost and reliable in result and has a wide application prospect range in early screening diagnose of diseases of stomach cancer and the like for mass groups.

Description

technical field [0001] The invention specifically relates to a saliva diagnostic sensor and its preparation method and application, such as its application in detecting gastric cancer markers in saliva and diagnosing gastric cancer, so as to facilitate the early screening and diagnosis of gastric cancer, belonging to saliva diagnosis and clinical detection field. Background technique [0002] Gastric cancer is one of the most common malignant cancers today, and its morbidity and mortality rank the second and third among all cancers in China, respectively. At present, the best way to treat gastric cancer is early diagnosis. Statistics show that the 5-year survival rate of early gastric cancer can reach 90%, while that of advanced gastric cancer is less than 24%. However, 80% of gastric cancers have very inconspicuous symptoms in the early stage, and are easily overlooked or missed diagnosis. When it can be diagnosed clinically, gastric cancer has often developed to the mid...

Claims

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Application Information

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IPC IPC(8): G01N21/65B82Y30/00B82Y40/00
CPCG01N21/658B82Y30/00B82Y40/00
Inventor 刘党培刘岩磊
Owner 苏州纳葛诺斯生物科技有限公司
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