Compound based on fish roe active substances of flatfish
An active substance and flounder technology, which is applied in the field of complexes based on flounder roe active substances, can solve the problems of fishy and bitter taste, short phase separation time, etc., and achieves excellent taste and flavor, no organic solvent residue, selection good effect
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Embodiment 1
[0020] The compound based on the active substance of flounder fish roe, its ingredients and parts by weight are: 8 parts of flounder fish roe active substance, 20 parts of fish protein powder, 18 parts of maltodextrin, 6 parts of bone meal, 1 part of bioactive calcium, lemon 5 parts of acid, 7 parts of glucose, 8 parts of honey, and 1 part of vitamin.
[0021] The preparation method of the compound based on the active substance of flatfish roe includes degreasing, dephosphorization, enzymatic hydrolysis of protease, deodorization and bitterness removal, purification and separation, and preparation of the compound. The specific steps are:
[0022] 1) Degreasing: Put the fish egg powder in the extraction kettle, under the pressure of 32MPa and the temperature of 48℃, pass CO 2 Carry out defatting for 90min, centrifuge at 5000r / min for 10min, remove the supernatant to obtain defatted fish egg powder, and use supercritical CO 2 Fluid degreasing is a new green separation technolog...
Embodiment 2
[0029] The specific steps of the preparation method of the compound based on the flounder fish roe active substance are:
[0030] 1) Put the fish egg powder in the extraction kettle, under the pressure of 32MPa and the temperature of 48°C, pass CO 2 Carry out defatting for 85 minutes, centrifuge, and remove the supernatant to obtain defatted fish egg powder, and use supercritical CO 2 Fluid degreasing is a new green separation technology, which avoids the problems of high temperature in traditional processing and damage to sample components. It has the advantages of high degreasing rate, good selectivity, and no organic solvent residue. Preparation of active substances, and the quality of the removed fish oil is not oxidized and can be further utilized;
[0031] 2) Dephosphorize the defatted fish egg powder with a NaOH solution with a concentration of 0.11mol / L for 115 minutes, adjust the pH to neutral, centrifuge, and remove the supernatant to obtain the dephosphorized fish ...
Embodiment 3
[0037] The compound based on the active substance of flounder fish roe, its ingredients and parts by weight are: 10 parts of flounder fish roe active substance, 19 parts of fish protein powder, 18 parts of maltodextrin, 8 parts of bone meal, 1.4 parts of living calcium, lemon 3.5 parts of acid, 8 parts of glucose, 9 parts of honey, and 1.2 parts of vitamins.
[0038] The preparation method of the compound based on the flounder fish roe active substance, the steps are: the fish roe powder is placed in the extraction kettle, and at a pressure of 32MPa and a temperature of 50°C, CO 2 Degrease for 90 minutes, centrifuge; dephosphorize the precipitated substance with NaOH solution with a concentration of 0.11mol / L for 110 minutes, adjust the pH to neutral, and centrifuge; add water to the precipitated substance according to the ratio of material to liquid at 1:9, mix well, and adjust the pH value To 8.8, add trypsin 1800U / g, alkaline protease 1200U / g, enzymolyze at 50°C for 6h, ina...
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