A method for synthesizing noble metal nanoparticles using exopolysaccharide of Lactobacillus plantarum

A technology of Lactobacillus plantarum and extracellular polysaccharides, which is applied in the fields of biotechnology and nanomaterials, can solve the problems of energy consumption, environmental pollution, and time-consuming synthesis of precious metal nanoparticles, and achieve safe and harmless raw material sources, uniform size distribution, and reduced The effect of energy consumption

Active Publication Date: 2019-12-10
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a method for synthesizing noble metal nanoparticles using exopolysaccharide as a reducing agent, so as to solve the problems of energy consumption, time consumption and environmental pollution in the synthesis of noble metal nanoparticles

Method used

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  • A method for synthesizing noble metal nanoparticles using exopolysaccharide of Lactobacillus plantarum
  • A method for synthesizing noble metal nanoparticles using exopolysaccharide of Lactobacillus plantarum
  • A method for synthesizing noble metal nanoparticles using exopolysaccharide of Lactobacillus plantarum

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Embodiment 1

[0034] The preparation method of embodiment 1 exopolysaccharide

[0035] A sample of Lactobacillus plantarum LCC-605 was taken and activated three times with MRS medium. Centrifuge at 14000g for 30min at 4°C to remove bacteria. After centrifugation, pour the upper clear night into a beaker, and centrifuge the remaining cells again, pour the upper clear night into the beaker again, and then pour 80% trichloroacetic acid into the beaker until the final concentration of trichloroacetic acid is 4%. Refrigerate overnight at 4°C to precipitate protein. The next day, the solution in the beaker was centrifuged again to separate the precipitated protein. Remove the precipitate, pour out the supernatant, then add three times the volume of absolute ethanol, and put it in a refrigerator at 4°C overnight to precipitate the exopolysaccharide. The next day, centrifuge the solution in the beaker to precipitate polysaccharides. Take out the polysaccharide and add a small amount of ultrapur...

Embodiment 2

[0036] Example 2 Exopolysaccharide Synthesis of Noble Metal Nanoparticles

[0037] Make exopolysaccharide into a 2mg / mL solution, take 5mL and add it to 50mL 100mg / L chloroauric acid solution or silver nitrate solution, and boil. Samples were taken for analysis at 0, 3, 10, 15 and 30 minutes, respectively. see results figure 1 , Wine red appeared in the chloroauric acid polysaccharide solution at 15 minutes, which is the characteristic color of gold nanoparticles, indicating that gold nanoparticles were synthesized after 15 minutes of reaction.

Embodiment 3

[0038] Example 3 Exopolysaccharide Synthesis of Noble Metal Nanoparticles

[0039] Make exopolysaccharide into a 0.2mg / mL solution, take 5mL and add it to 25mL 10mg / L chloroauric acid solution or silver nitrate solution, react at 25°C for 40min and take samples for analysis.

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Abstract

The invention discloses a method for synthesizing precious metal nano-particles with extracellular polysaccharide obtained by fermenting lactobacillus plantarum. The lactobacillus plantarum extracellular polysaccharide and a precious metal solution are used as raw materials, and the precious metal nano-particles can be obtained by means of reacting. The synthesized precious metal nano-particles are uniform in grain size and good in dispersity. In addition, the method is short in reaction time and low in energy consumption, no chemical reagent needs to be added, and green synthesis of the precious metal nano-particles is realized.

Description

technical field [0001] The invention belongs to the field of biotechnology and nanometer materials, and in particular relates to the application of plant lactobacillus exopolysaccharide in synthesizing noble metal nanoparticles. Background technique [0002] The traditional synthesis method of noble metal nanoparticles mostly adopts chemical reagent reduction method, which involves chemical reducing agents such as ethylenediamine and sodium borohydride, and their use brings potential environmental pollution and biological toxicity and other problems. Therefore, people began to search for alternative methods to synthesize noble metal nanoparticles to achieve green and sustainable development. Polysaccharides and their derivatives have been proven to be used to synthesize noble metal nanoparticles, including lentinan, carboxymethyl chitosan, pullulan, dextran, carboxymethyl cellulose, guar gum, etc. However, there are certain defects when using these polysaccharides and their...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B22F9/24B82Y40/00
CPCB22F9/24B82Y40/00
Inventor 林凤鸣李程程周乐
Owner SOUTHEAST UNIV
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