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Novel polypeptide PAP1 and application thereof

A technology for new peptides and supplies, applied in the new peptide PAP1 and its application fields, can solve the problems of high preparation cost and poor stability, and achieve the effects of promoting migration, easy synthesis, and easy mass production

Active Publication Date: 2017-10-20
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, peptides such as epidermal growth factor (hEGF) and basic fibroblast growth factor (bFGF) have obvious effects of repairing wounds, skin care, anti-wrinkle and anti-aging, but their high preparation cost and poor stability limit their application.

Method used

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  • Novel polypeptide PAP1 and application thereof
  • Novel polypeptide PAP1 and application thereof
  • Novel polypeptide PAP1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Synthesis of new polypeptide PAP1

[0029] Using the conventional solid-phase synthesis method of an automatic peptide synthesizer, the synthesis of the new peptide PAP1 was completed through resin swelling, deprotection, washing, amino acid dissolution, amino acid activation, and condensation (entrusted to Hefei Guopeptide Biotechnology Co., Ltd. to synthesize).

Embodiment 2

[0030] Example 2 Structural Identification of Polypeptide PAP1

[0031] The new polypeptide PAP1 that embodiment 1 makes is white powder; UV (MeOH) λ max (logε)205(3.85),224(1.61),279(0.12)nm; IR(KBr)ν max 3300, 2968, 2889, 1664, 1537, 1447, 1205, 1132, 801, 722cm -1 ;HR-ESI-MS m / z 1447.6919[M+H] + (C 66 h 94 N 16 o 21 , calculated value 1447.6852). Hydrogen spectrum ( 1 H NMR) and carbon spectrum ( 13 C NMR) data in Table 1 (assigned by 2D-NMR). The amino acid sequencing result was: H-Ser-Ser-Pro-Tyr-Thr-Ser-Gly-Pro-His-Pro-Gly-Val-Val-Tyr-OH. That 1 H, 13 C NMR spectrum see Figure 1~2 ; high-resolution mass spectrometry see image 3 . According to the above physicochemical and spectral data, the chemical structure of PAP1 was identified, as shown in Formula I.

[0032] Table 1 PAP1 1 H(500MHz) and 13 C (125MHz) NMR data

[0033]

[0034]

[0035] a) Measured at 500MHz. b) Measured at 125MHz. c) Overlapped signals were reported without designatin...

Embodiment 3

[0036] Example 3 Promoting effect of new polypeptide PAP1 on the proliferation of Balb / c 3T3 cells

[0037] Cells in the logarithmic growth phase (Balb / c 3T3 cell line, purchased from the American Type Culture Collection, ATCC) were digested with trypsin, centrifuged, and resuspended for cell counting, counted as 3000 cells Inoculate each well in a 96-well plate, tap until the cells are evenly dispersed, and block with PBS. 37°C, 5% CO 2 Incubate overnight. Abandon the medium, add starvation medium containing 0.5% FBS by volume to treat for 24h, and synchronize the cells; discard the starvation medium, add complete medium to configure different concentrations of polypeptide PAP1 (prepared in Example 1), 37 ° C, 5 %CO 2 Incubator for 48h. Add CCK8 reagent at 10 μL / well, incubate at 37°C for 3 h, and detect with dual wavelengths of 450 nm and 630 nm on a microplate reader. Calculate the cell survival rate and repeat the experiment at least 3 times;

[0038] Test results su...

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Abstract

The invention belongs to the field of medicines and daily chemicals and particularly relates to a novel polypeptide PAP1 and an application thereof. The novel polypeptide PAP1 has the structure shown in the formula I. The novel polypeptide can promote proliferation of embryo fibroblasts of mice and migration of immortalized human epidermal cells at relatively low concentration, has low toxic and side effects, has good application prospects and can be used for preparation of medicines for treating wounds, scalds and ulcers or cosmetics and daily chemicals for improving skin beauty.

Description

technical field [0001] The invention belongs to the field of medicine and daily chemicals, and in particular relates to a new polypeptide PAP1 and its application. Background technique [0002] The healing process after tissue trauma is a process of proliferation, differentiation, migration and apoptosis of various repair cells. Acute and chronic injuries such as inflammation, ulcers, burns, trauma, surgery, and congenital deformities can cause large areas of skin defects, refractory ulcers, and scars, and trigger many local and systemic reactions, causing serious harm. Peptide drugs have unique advantages in promoting wound healing, preventing scars and treating chronic ulcers. Among them, peptides such as epidermal growth factor (hEGF) and basic fibroblast growth factor (bFGF) have obvious effects of repairing wounds, skin care, anti-wrinkle and anti-aging, but their high preparation cost and poor stability limit their application. . Contents of the invention [0003]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08A61K8/64A61Q19/02A61Q19/08A61K38/10A61P1/04A61P1/02A61P17/02C11D9/30
CPCA61K8/64A61K38/00A61Q19/02A61Q19/08C07K7/08C11D9/30
Inventor 叶文才王磊张薇
Owner JINAN UNIVERSITY
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