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Application of Japanese raspberry root or Japanese raspberry root extract to preparation of lipid-lowering drug

A technology of Tianqingdibai and its extracts, which is applied in the direction of drug combination, medical formula, plant raw materials, etc., can solve the problems of no heat-clearing and detoxifying, cannot replace Tianqingdibai's lipid-lowering function, and different clinical application focuses, etc., to achieve No toxic side effects, low cost, clear effect mechanism

Inactive Publication Date: 2017-11-03
CAPITALBIO CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cui Jue et al.’s paper “Studies on the Effects of Total Flavonoids of Gnaphus Grass on Improving Blood Lipid Metabolism Disorders in Diabetic Mice”, reported the blood lipid-lowering effect of Gnaphalium offine, a plant belonging to the same genus of Gnaphium offine. Functions: S. vermicelli and A. chinensis belong to the genus S. genus of Asteraceae, but they are not of the same species. The two have different properties, flavors, meridian distribution and efficacy. S. versicolor is sweet in nature, mild in flavor , return to the lung meridian, vermicelli relieving cough and asthma, lowering blood pressure, dispelling rheumatism, used for cold cough, bronchitis, asthma, hypertension, favism, rheumatic lumbago and leg pain; external use for bruises, snake bites, no The efficacy of heat-clearing and detoxifying, it can be seen that even though the two plants belong to the genus Salmonella, they have different clinical uses, so it is speculated that the lipid-lowering function of vermicelli can not replace the lipid-lowering function of Tianqingdibai
Therefore, there are no relevant reports about the lipid-lowering function of Tianqingdibai single herbal medicine and compound medicine.

Method used

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  • Application of Japanese raspberry root or Japanese raspberry root extract to preparation of lipid-lowering drug
  • Application of Japanese raspberry root or Japanese raspberry root extract to preparation of lipid-lowering drug
  • Application of Japanese raspberry root or Japanese raspberry root extract to preparation of lipid-lowering drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] In the embodiment of the present invention, the effect of Tianqingdibai on genes related to lipid metabolism was detected by fluorescent real-time quantitative PCR technology (RT-qPCR), and it was confirmed that Tianqingdibai has a lipid-lowering effect. Among them, the genes of focus are LDLR, MTP, APOB (specifically referring to APOB expressed in the liver 100 ) and DGAT1.

[0036] The gene LDLR, low-density lipoprotein receptor, is located on the liver cell membrane and mediates the clearance of LDL in the blood through endocytosis. More than 75% of LDL in the blood is cleared through LDLR.

[0037] MTP, microsomal triacylglycerol transporter, transports triglycerides, cholesteryl esters and phospholipids to the newly formed APOB, which assembles into very low-density lipoprotein particles (VLDL).

[0038] APOB, apolipoprotein B, is produced by the liver and is the exclusive component of nascent VLDL. VLDL is assembled in the liver cells, secreted into the circulation...

Embodiment 2

[0060] The embodiment of the present invention compares the effects of Gnaphalium japonicum and other two plants of the same genus, Gnaphalium offine and Gnaphalium pensylvanicum on the above-mentioned genes, and the test method is the same as in Example 1 . Among them, the extraction method of the Vermicelli extract and S. sativa is the same as the extraction method of Azurite, which is an alcohol extract, and the working concentration is 100 μg / mL. qPCR results ( figure 2 ) shows that the effect of Azure Dibai is significantly stronger than that of the other two medicinal materials, especially that Azure Dibai can up-regulate the LDLR gene, while the other two medicinal materials can down-regulate the LDLR gene, so the present invention proves that Azure Dibai has a strong lipid-lowering effect In vermicelli and spoon leaf sage.

Embodiment 3

[0062] The embodiment of the present invention uses the detection of the absorption of fluorescently labeled low-density lipoprotein to verify the effect of Tianqingdibai, and the process is as follows:

[0063] 1. Control DMSO and azure diba extract (100 μg / mL) to treat the cells for 24 hours.

[0064] 2. Discard the medium containing the drug, rinse the cells twice with PBS, add serum-free DMEM medium, and add DiI-LDL (20 μg / mL) to continue incubation for 4 hours;

[0065] 3. The cells were collected by trypsinization, and the content of DiI-LDL in the cells was detected by flow cytometry (emission: 541nm; excitation: 575nm).

[0066] Stream results see image 3 , the results show that Azure Dibai can promote the absorption capacity of cells to LDL, that is, promote the clearance of LDL in the circulatory system.

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Abstract

The invention relates to the technical field of medicine use and particularly relates to an application of a Japanese raspberry root or a Japanese raspberry root extract to preparation of a lipid-lowering drug. The research result of the application shows that the Japanese raspberry root obviously regulates up a gene LDLR and obviously regulates down a gene MTP, a gene APOB and a gene DGAT1 on the gene level; the lipid lowering effect of the Japanese raspberry root is stronger than that of two same generic plants of a cudweed and a gnaphalium pensylvanicum. The result shows that the Japanese raspberry root promotes the absorbing capability of LDL of a cell on the cell level, that is, the clearing of the LDL in a circulating system is prompted; the content of triglyceride in the cell is reduced. The result shows that the Japanese raspberry root or the Japanese raspberry root extract has a remarkable effect of lowering lipid, the mechanism of action is clear, the cost of the Japanese raspberry root is low, the source of the Japanese raspberry root is wide, the preparation is simple, and the Japanese raspberry root has no toxic and / or side effects.

Description

technical field [0001] The invention relates to the technical field of medicine application, in particular to the application of Tianqingdibai or its extract in the preparation of lipid-lowering medicine. Background technique [0002] Hyperlipidemia refers to excessive blood lipid levels, specifically refers to blood cholesterol (TC, LDL-c) and / or triglyceride (TG) is too high or high-density lipoprotein cholesterol (HDL-C) is too low. The clinical manifestations are mainly xanthoma caused by lipid deposition in the dermis and arteriosclerosis caused by lipid deposition in the vascular endothelium. Hyperlipidemia can directly cause some diseases that seriously endanger human health, such as atherosclerosis, coronary heart disease, pancreatitis, etc. 30 million people in the world die every year from related diseases caused by hyperlipidemia, and the most serious one is cardiovascular and cerebrovascular diseases. disease. With the improvement of living standards, the morbi...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61P3/06
CPCA61K36/28A61K2236/333A61K2236/51
Inventor 冯娟谢兰郭弘妍邢婉丽程京
Owner CAPITALBIO CORP
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