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A plant lectin-polysaccharide hydrogel that intelligently regulates insulin release and its preparation and application

A plant lectin and hydrogel technology, which can be applied to medical preparations without active ingredients, medical preparations containing active ingredients, drug combinations, etc. and other problems, to achieve the effect of maintaining structural stability and biological activity, simple process, and great application prospects

Active Publication Date: 2021-04-02
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a new type of degradable medical polymer material, polyphosphazene has been widely used in recent years, but its degradation products are not all non-toxic, and there are certain safety hazards; The reaction conditions will affect the activity of lectins on glucose, thus affecting the final use effect

Method used

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  • A plant lectin-polysaccharide hydrogel that intelligently regulates insulin release and its preparation and application
  • A plant lectin-polysaccharide hydrogel that intelligently regulates insulin release and its preparation and application
  • A plant lectin-polysaccharide hydrogel that intelligently regulates insulin release and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] 1. Preparation of insulin-loaded concanavalin-pullulan hydrogel

[0062] (1) Weigh 0.2 g of pullulan polysaccharide, named PUL, put it in 20 ml of dimethyl sulfoxide, dissolve and stir at 15°C for 2 hours;

[0063] (2) Weigh 3 mg of succinic anhydride, place in 1 ml of dimethyl sulfoxide, and dissolve at 15°C for 4 hours;

[0064] (3) Add the solution obtained in step (2) to the mixed solution obtained in step (1), then add 16 mg of triethylamine dropwise to the mixed solution, and stir and react at 15°C for 1 hour. After the reaction, the reaction solution was dialyzed with distilled water in a dialysis bag with a cut-off molecular weight of 1,000, and freeze-dried at -20°C to obtain a carboxylated polysaccharide derivative, which was named CPUL;

[0065] (4) Weigh 0.3 g of the CPUL obtained in step (3), dissolve it in 2 ml of acetic acid-sodium acetate buffer solution (pH 4.0), stir and dissolve at 15°C for 12 hours;

[0066] (5) Weigh 0.01 mg of magnesium chloride,...

Embodiment 2

[0080] 1. Preparation of insulin-loaded peanut agglutinin-pullulan hydrogel

[0081] (1) Weigh 0.5 g of amylopectin, name it AMYP, and place it in 50 ml N,N - in dimethylformamide, dissolved and stirred at 30°C for 24 hours;

[0082] (2) Weigh 5 mg of malonic anhydride and place in 5 ml N,N - in dimethylformamide, dissolved at 30°C for 24 hours;

[0083] (3) Add the solution obtained in step (2) to the mixed solution obtained in step (1), then add 10 mg of diethylamine dropwise to the mixed solution, and stir and react at 30°C for 24 hours. After the reaction, the reaction solution was dialyzed with distilled water in a dialysis bag with a cut-off molecular weight of 50,000, and freeze-dried at -30°C to obtain a carboxylated polysaccharide derivative, which was named CAMYP;

[0084] (4) Weigh 0.5 g of CAMYP obtained in step (3), dissolve in 5 ml of disodium hydrogen phosphate-sodium dihydrogen phosphate buffer (pH7.4), and dissolve at 30°C for 24 hours;

[0085] (5) Weigh ...

Embodiment 3

[0092] 1. Preparation of chickpea lectin-guar gum hydrogel loaded with insulin

[0093] (1) Weigh 0.8 g of guar gum, name it GUA, place it in 40 ml of formamide, stir and dissolve at 35°C for 15 hours;

[0094] (2) Weigh 10 mg of succinic anhydride, place in 10 ml of formamide, and dissolve at 35°C for 12 hours;

[0095] (3) Add the solution obtained in step (2) to the mixed solution obtained in step (1), then add 15 mg of triethylamine dropwise to the mixed solution, and react at 35°C for 12 hours. After the reaction, the reaction solution was dialyzed with distilled water in a dialysis bag with a cut-off molecular weight of 10,000, and freeze-dried at -60°C to obtain a carboxylated polysaccharide derivative, which was named CGUA;

[0096] (4) Weigh 0.1 g of CGUA obtained in step (3), dissolve it in 1 ml of citric acid-sodium hydroxide-hydrochloric acid buffer solution (pH5.3), stir and dissolve at 35°C for 10 hours;

[0097] (5) Weigh 0.05 mg of magnesium chloride, 0.05 mg...

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Abstract

The invention discloses intelligent insulin release regulation type phytolectin-polysaccharide hydrogel and preparation and application thereof. The phytolectin-polysaccharide hydrogel in a porous structure is prepared from natural polysaccharides high in biocompatibility and degradability through chemical crosslinking with phytolectin under the action of a pore-forming agent. The phytolectin-polysaccharide hydrogel can be used for loading insulin to realize preparation of the polysaccharide hydrogel with a function of intelligent insulin release regulation. The preparation conditions are mild, adopted raw materials are safe, activity of phytolectin to glucose molecule specific binding and structural stability and bioactivity of insulin are kept, the process is simple, and adopted equipment and raw materials are cheap and easy to acquire. The intelligent insulin release regulation type phytolectin-polysaccharide hydrogel is hopefully used as an insulin delivery carrier for intelligent insulin release regulation and has a promising application prospect in diabetes treatment.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials. More specifically, it relates to a plant lectin-polysaccharide hydrogel capable of intelligently regulating insulin release, its preparation method and application. Background technique [0002] Diabetes is a disease with a high incidence rate. At present, the treatment of diabetes mainly relies on multiple daily subcutaneous injections of insulin. However, this method cannot intelligently regulate the release of insulin according to the patient's blood sugar level. Therefore, multiple injections of insulin are required. Too much insulin can cause hypoglycemia, an acute complication, and even fainting and shock in severe cases. At the same time, long-term insulin injections can also lead to skin infections, subcutaneous blood stasis and other problems. [0003] Smart materials have received extensive attention in recent years, also known as environmental responsive materials, such a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/06A61K47/42A61K47/36A61K38/28A61P3/10
CPCA61K9/06A61K38/28A61K47/36A61K47/42
Inventor 林坤华易菊珍杨立群吴昊
Owner SUN YAT SEN UNIV
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