Preparation method of heterogeneous acellular dermal matrix

A decellularized dermis and matrix technology, which is applied in the field of preparation of heterogeneous acellular dermal matrix, can solve the problems of collagen scaffolds losing environmental protection, inability to effectively remove heterogeneous skin cells, and reduce the antigenicity of heterogeneous skin, so as to promote wound healing, Improve adherence and improve application performance

Active Publication Date: 2017-12-01
GUANGZHOU RAINHOME PHARM&TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing acellular dermal matrix production process has the following deficiencies: it cannot effectively remove xenogeneic skin cells, reduce the antigenicity of xenogeneic skin while retaining the complete three-dimensional structure of dermal collagen fibers; during the preparation of acellular dermal matrix, the hyaluronic Extracellular amorphous matrix components suc

Method used

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  • Preparation method of heterogeneous acellular dermal matrix
  • Preparation method of heterogeneous acellular dermal matrix
  • Preparation method of heterogeneous acellular dermal matrix

Examples

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Effect test

Embodiment 1

[0029] A method for preparing a heterogeneous acellular dermal matrix, comprising the following steps:

[0030] (1) Select Bamaxiang pig skin as the skin source, depilate and sterilize the skin extraction area, remove large pieces of skin with a skin extractor, wash with sterile saline, and reverse the skin drum to obtain a 0.30mm slice of skin, Using laser drilling technology to make holes in the tomographic skin, spare;

[0031](2) Immerse the slice obtained in step (1) in an aqueous solution of sodium o-phenylphenolate containing 0.02% (v / v) dipropylene glycol dimethyl ether with a concentration of 0.8% (m / v), and treat 6min, washed 3 times with sterile saline, and then added 2% (m / v) sodium tripolyphosphate, 0.02% (v / v) dipropylene glycol dimethyl ether and 0.2% (m / v) polyethylene glycol 15-Hydroxystearate, the concentration is 2% (m / v) trimethylolmethylaminoethanesulfonic acid aqueous solution, ultrasonically vibrated for 8h, and the solution was replaced every 2h. The ...

Embodiment 2

[0037] A method for preparing a heterogeneous acellular dermal matrix, comprising the following steps:

[0038] (1) Select Bamaxiang pig skin as the skin source, depilate and sterilize the skin extraction area, remove large pieces of skin with a skin extractor, wash with sterile saline, and reverse the skin drum to obtain a 0.35mm sliced ​​skin, Using laser drilling technology to make holes in the tomographic skin, spare;

[0039] (2) Immerse the slice obtained in step (1) in an aqueous solution of sodium o-phenylphenolate containing 0.04% (v / v) dipropylene glycol dimethyl ether with a concentration of 0.6% (m / v), and process 8min, washed 4 times with sterile saline, and then added 3% (m / v) sodium tripolyphosphate, 0.02% (v / v) dipropylene glycol dimethyl ether and 0.1% (m / v) polyethylene glycol 15-Hydroxystearate, the concentration is 2% (m / v) trimethylolmethylamine ethanesulfonic acid aqueous solution, ultrasonically vibrated for 12h, and the solution was replaced every 2h. ...

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Abstract

The invention relates to a preparation method of heterogeneous acellular dermal matrix. The preparation method comprises the following steps: taking bama miniature pig skin as a skin source, reversely taking split-thickness skin by adopting a skin drum, carrying out the steps of removing epidermis and extracting cells, thus obtaining acellular dermis, enabling the acellular dermis to react with carboxymethyl chitosan, thus obtaining acellular dermal matrix, finally, treating the acellular dermal matrix by adopting a softening agent and a humectant, so that the prepared acellular dermal matrix is closer to the natural state of the skin of the human body, and thus the use compliance of the acellular dermal matrix is improved. The heterogeneous acellular dermal matrix prepared by adopting the method disclosed by the invention is low in immunogenicity, meanwhile, the heterogeneous acellular dermal matrix keeps the three-dimensional stereoscopic structure of dermal collagen fibers, improves the stability of the three-dimensional structure of the dermal collagen fibers, and has the excellent biocompatibility.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering skin, and in particular relates to a preparation method of a heterogeneous acellular dermal matrix. Background technique [0002] Skin defects caused by factors such as burns, trauma, epithelial cancer resection, and skin diseases often leave scar hyperplasia and contracture deformities, which seriously affect the appearance and function. At present, in addition to autologous skin and skin flap, the repair materials used to repair wounds mainly include allogeneic skin, xenograft and various synthetic wound coverings. In the treatment of patients with large-area burns, the patient's autologous skin source is limited, and these skin substitutes need to be used; the source of allogeneic skin is limited, and there is the risk of infectious diseases, and it is restricted by ethics; artificial synthetic material technology is still immature, anti-inflammatory Infectivity is poor and expensiv...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/50A61L27/54A61L27/60
CPCA61L27/362A61L27/3687A61L27/3691A61L27/50A61L27/54A61L27/60A61L2300/404A61L2300/412A61L2430/40
Inventor 车七石
Owner GUANGZHOU RAINHOME PHARM&TECH CO LTD
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