Capturing method for organic exhaust gas
A technology of organic waste gas and inoculum, applied in the direction of separation methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems that cannot be effectively realized, ignored, and facultative and anaerobic bacteria in the deep part of the oil reservoir cannot be fully obtained Activation and other issues, to achieve good treatment, promote the effect of degradation
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Embodiment 1
[0043] A method for capturing organic waste gas, comprising the following steps:
[0044] (1) Preparation of methanogenic inoculum: pump the oil-water mixture from the oil production wellhead, and store the collected oil-water mixture in a sealed container at 4°C. Immediately carry out enrichment culture after the collection is completed; the specific operation of enrichment culture is to inject 50 mL of the oil-water mixture into the In a 120mL sterile anaerobic bottle, the Hungate device was used to feed high-purity nitrogen gas to remove the oxygen remaining in the bottle, and then the bottle was closed with a rubber stopper to make it in a strict anaerobic state. And place it in a constant temperature incubator for dark culture. After 100 days, use high-pressure liquid phase detection to analyze the headspace gas. When methane is detected, use the liquid in the bottle as the methanogenic inoculum for enrichment culture;
[0045] (2) Preparation of inorganic salt medium: KH...
Embodiment 2
[0049] A method for capturing organic waste gas, comprising the following steps:
[0050] (1) Preparation of methanogenic inoculum: pump the oil-water mixture from the oil production wellhead, and store the collected oil-water mixture in a sealed container at 4°C. Immediately carry out enrichment culture after the collection is completed; the specific operation of enrichment culture is to inject 50 mL of the oil-water mixture into the In a 120mL sterile anaerobic bottle, the Hungate device was used to feed high-purity nitrogen gas to remove the oxygen remaining in the bottle, and then the bottle was closed with a rubber stopper to make it in a strict anaerobic state. And place it in a constant temperature incubator for dark culture. After 100 days, use high-pressure liquid phase detection to analyze the headspace gas. When methane is detected, use the liquid in the bottle as the methanogenic inoculum for enrichment culture;
[0051] (2) Preparation of inorganic salt medium: KH...
Embodiment 3
[0055] A method for capturing organic waste gas, comprising the following steps:
[0056] (1) Preparation of methanogenic inoculum: pump the oil-water mixture from the oil production wellhead, and store the collected oil-water mixture in a sealed container at 4°C. Immediately carry out enrichment culture after the collection is completed; the specific operation of enrichment culture is to inject 50 mL of the oil-water mixture into the In a 120mL sterile anaerobic bottle, the Hungate device was used to feed high-purity nitrogen gas to remove the oxygen remaining in the bottle, and then the bottle was closed with a rubber stopper to make it in a strict anaerobic state. And place it in a constant temperature incubator for dark culture. After 100 days, use high-pressure liquid phase detection to analyze the headspace gas. When methane is detected, use the liquid in the bottle as the methanogenic inoculum for enrichment culture;
[0057] (2) Preparation of inorganic salt medium: KH...
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