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Method for preparing sunflower seed meal protein

A technology for sunflower seed meal and protein is applied in the field of sunflower seed meal protein preparation, which can solve the problems of protein denaturation, long production cycle, long time consumption, etc., and achieve the effects of mild reaction conditions, no destructive effect, and improved removal rate.

Inactive Publication Date: 2017-12-05
WUXI JINNONG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the main method for removing chlorogenic acid is solvent removal method. The solvent is mainly ethanol. Chlorogenic acid and some reducing sugars, but alcohol has a certain denaturation effect on protein; microwave and ultrasonic technology to remove chlorogenic acid, limited by the amount of treatment, is currently limited to laboratory scale; although the removal process by water immersion extraction is simple and cost-effective low, but the production cycle is long and the energy consumption is large; although the macroporous resin method has a remarkable extraction effect, it is difficult to clean the adsorption resin and takes a long time, so its promotion is limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A preparation method of sunflower meal protein, the method comprises the following specific steps:

[0026] (1) Pulverize the dehulled defatted sunflower seed meal with a pulverizer, and then pass through a 60-mesh sieve to remove impurities to obtain pulverized material;

[0027] (2) Mix the crushed sunflower seed meal with water at a mass ratio of 1:8, add cellulase (0.1% of the dry basis of the crushed sunflower seed meal, and react at 45°C for 3 hours. After the reaction, 4000r / min Centrifuge for 10 minutes at a high speed to obtain a precipitate, and dry it at 50°C for later use;

[0028] (3) Mix the precipitated product prepared in step (2) with NaCl solution (0.5mol / L) at a mass ratio of 1:8, stir evenly, and react at 45°C for 3 hours. After the reaction, centrifuge to take the supernatant solution, and use 1mol / L hydrochloric acid to adjust the pH of the supernatant to 4.0, let it stand for 2h, centrifuge at 4000r / min for 10min to obtain the precipitate, then a...

Embodiment 2

[0033] A preparation method of sunflower meal protein, the method comprises the following specific steps:

[0034] (1) Pulverize the dehulled defatted sunflower seed meal with a pulverizer, and then pass through an 80-mesh sieve to remove impurities to obtain pulverized material;

[0035] (2) Mix the crushed sunflower seed meal with water at a mass ratio of 1:10, add cellulase (0.3% of the dry basis of the crushed sunflower seed meal, and react at 50°C for 2 hours. After the reaction, 4500r / min Centrifuge for 7 minutes at a high speed to obtain a precipitate, and dry it at 55°C for later use;

[0036] (3) Mix the precipitated product prepared in step (2) with NaCl solution (1.0mol / L) at a mass ratio of 1:10, stir evenly, and react at 50°C for 2 hours. After the reaction, centrifuge to take the supernatant liquid, and use 1mol / L hydrochloric acid to adjust the pH of the supernatant to 4.5, let it stand for 1.5h, centrifuge at 4500r / min for 7min to obtain a precipitate, then ad...

Embodiment 3

[0041] A preparation method of sunflower meal protein, the method comprises the following specific steps:

[0042] (1) Pulverize the dehulled defatted sunflower seed meal with a pulverizer, and then pass through a 100-mesh sieve to remove impurities to obtain pulverized material;

[0043] (2) Mix the crushed sunflower seed meal with water at a mass ratio of 1:12, add cellulase (0.5% of the dry basis of the crushed sunflower seed meal, and react at 55°C for 1 hour. After the reaction, 5000r / min Centrifuge for 5 minutes at a high speed to obtain a precipitate, and dry it at 60°C for later use;

[0044](3) Mix the precipitated product prepared in step (2) with NaCl solution (1.5mol / L) at a mass ratio of 1:12, stir evenly, and react at 55°C for 1 hour. After the reaction, centrifuge to take the supernatant solution, and use 1mol / L hydrochloric acid to adjust the pH of the supernatant to 5.0, let it stand for 1h, centrifuge at 5000r / min for 5min to obtain the precipitate, then adj...

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Abstract

The present invention discloses a method for preparing sunflower seed meal protein, and the method comprises the following specific steps: (1) raw material processing; (2) removal of sunflower seed meal chlorogenic acid; (3) preparation of sunflower seed meal protein; (4) homogenizing; (5) spray drying; and (6) packaging to prepare the sunflower seed meal protein. The method adopts a cellulose method to remove the chlorogenic acid in sunflower seed meal and has a high removal rate. According to the characteristics of the raw material protein, sunflower seed globulin is extracted by a method of salt extraction and acid precipitation, and the obtained globulin has good emulsifying, emulsifying stability, foaming and foaming stability, and can be used as a food additive in food processing.

Description

technical field [0001] The invention relates to the technical field of bioprocessing, in particular to a method for preparing sunflower seed meal protein. Background technique [0002] Sunflower meal refers to the substance obtained by pre-pressing or direct leaching of sunflower seeds to extract oil. Loose flaky, powdery; has the characteristic gray or gray-black color of sunflower seeds, and has the characteristic fragrance of sunflower seeds. Generally treated as animal feed or waste, the utilization rate is low, and its high added value has not been realized. However, the protein content in sunflower seed meal is 29-43%. Sunflower protein has a good distribution. Compared with the FAO value, its amino acid composition is higher than or similar to the FAO value except for lysine content. , although the lysine content is low, its effective lysine content can be as high as 90%. Sunflower protein is rich in sulfur-containing amino acids such as methionine. Compared with mo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/00A23J1/14
CPCA23J1/005A23J1/148A23V2002/00A23V2250/02A23V2250/1582A23V2250/1614A23V2250/548A23V2300/38A23V2300/40
Inventor 于秋生尹义敏陈天祥陈林李珍妮冯伟朱熹
Owner WUXI JINNONG BIOTECH CO LTD
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