Method for screening high-yield bacterial strain of vitamin B2
A technology of high-yield strains and screening methods, applied in the field of bioengineering, can solve the problems of unstable strains, large randomness, and no repeatability, and achieve the effects of reducing fermentation costs, improving synthesis capacity, and overcoming low yields
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[0025] A screening method for vitamin B2 high-yield strains, comprising the steps of:
[0026] a) Pretreatment of the original bacterial strain: 80 μl of the glycerol bacteria of the original bacterial strain of Bacillus subtilis (Bacillus subtilis) were evenly spread on the plate medium and cultivated at 37°C for 24 hours. The components of the plate medium were: yeast powder 5g / L, sodium chloride 10g / L, tryptone 10g / L, agar powder 18g / L, pH7.0; Bacteria, after mixing evenly, draw into a sterile centrifuge tube to obtain the bacterial liquid of the original bacterial strain of Bacillus subtilis, and set aside;
[0027] b) Take 2ml of the bacterial solution obtained in step a) and drop it on a sterile blank culture plate, and perform ultraviolet mutagenesis under sterile conditions for 30s to obtain an ultraviolet mutagenized bacterial strain;
[0028] c) Take 0.2ml of the bacterial liquid of the ultraviolet mutagenic strain obtained in step b), add it to 0.1mol / L ethyl meth...
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