A high-resolution conformation technique of genome-wide chromatin based on a small number of cells ehi-c 2.0

A chromatin and cell technology, applied in recombinant DNA technology, combinatorial chemistry, nucleotide library, etc., can solve the problems of poor data coverage and low interaction resolution.
CN107475394BActive Publication Date: 2021-06-15AGRI GENOMICS INST CHINESE ACADEMY OF AGRI SCI
3 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
AGRI GENOMICS INST CHINESE ACADEMY OF AGRI SCI
Publication Date
2021-06-15

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses eHi-C 2.0, a high-resolution conformation technology based on a small number of cells and whole genome chromatin. The invention provides a preparation method of a cell eHi-C 2.0 sequencing library: lyse cells to obtain chromatin, and then digest The chromatin; the digested DNA is sequentially labeled with a marker and blunt-ended to obtain a circularized product; introducing an internal reference circular co-precipitated DNA molecule into the circularized product; and then using a restriction exonuclease Enzyme digestion, then ultrasonic fragmentation, and then use immunomagnetic beads to grab the DNA fragments with the markers from the fragmentation products; use the DNA fragments with the markers to prepare eHi-C 2.0 sequencing library . The present invention can perform eHi-C library construction and sequencing on the number of cells as low as 0.1 million cells.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the technical field of genome sequencing, in particular to eHi-C 2.0, a genome-wide chromatin conformation technology based on a small number of cells. Background technique

[0002] Three-dimensional genomics is an emerging discipline that studies the three-dimensional spatial structure and function of the whole genome (chromatin) [Ruan Y, 2011, Science]; its research scope covers the visual analysis and simulation of the three-dimensional spatial structure of the genome. Genome interaction network research, chromatin dynamic domain, 3D data mining, 3D structure and signaling pathway function analysis, key gene or factor mining and other important research contents [Schmitt A.D, Ren B, 2016, NatureReviews Molecular Cell Biology]. In recent years, the 3D genomics technology based on chromatin conformation capture technology (Chromosome conformation capture) has been greatly developed, which can obtain 3D gene interaction from a ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More